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The map label for this gene is nfnB [C]

Identifier: 16273193

GI number: 16273193

Start: 1355217

End: 1355879

Strand: Reverse

Name: nfnB [C]

Synonym: HI1278

Alternate gene names: 16273193

Gene position: 1355879-1355217 (Counterclockwise)

Preceding gene: 16273199

Following gene: 16273191

Centisome position: 74.09

GC content: 41.93

Gene sequence:

>663_bases
ATGACTCAACTTACTCGTGAACAAGTTCTTGAACTCTTCCATCAACGCAGCTCAACACGTTATTACGACCCAACAAAAAA
AATCAGTGATGAAGATTTTGAATGTATTTTAGAGTGCGGTCGATTATCGCCGAGTTCTGTAGGCTCTGAGCCTTGGAAAT
TTTTAGTGATTCAAAATAAAACCTTACGCGAAAAAATGAAACCTTTTAGCTGGGGAATGATAAATCAGCTTGATAATTGC
AGTCATCTTGTGGTAATTCTCGCGAAGAAAAATGCCCGTTATGATAGTCCGTTTTTTGTGGATGTGATGGCACGCAAAGG
CTTGAACGCAGAGCAACAACAAGCCGCCCTCACAAAATACAAAGCCCTGCAAGAAGAAGATATGAAATTACTCGAAAACG
ACCGCACTTTATTTGATTGGTGCAGCAAACAAACTTATATCGCCCTTGCAAATATGCTTACTGGAGCTTCAGCCCTTGGC
ATCGACTCTTGCCCAATTGAAGGTTTTCATTACGACAAAATGAATGAATGCCTCGCCGAAGAAGGATTATTCGATCCTCA
AGAATATGCGGTTTCTGTCGCCGCAACCTTTGGCTATCGCTCACGCGATATTGCGAAAAAATCCCGTAAAGGATTGGATG
AAGTGGTGAAATGGGTGGGGTAA

Upstream 100 bases:

>100_bases
TGTGATAAAAGCGCTGACTATTCTAGAATCTTTCCCTTGTTTTAGCAAAAACTAATCTTTAATATGGCACCACATTTTTG
TTTAACAAAGAGGAAATAAA

Downstream 100 bases:

>100_bases
AAATCCCATTAAAAAAGGGCTGCACATGCAGCCCTATCTTTTTTGATTATTTCACTTCTTTAAATAAAATCTCGCTTGGG
ATTACGGAACCTTGCCAGTA

Product: NAD(P)H-flavin oxidoreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTQLTREQVLELFHQRSSTRYYDPTKKISDEDFECILECGRLSPSSVGSEPWKFLVIQNKTLREKMKPFSWGMINQLDNC
SHLVVILAKKNARYDSPFFVDVMARKGLNAEQQQAALTKYKALQEEDMKLLENDRTLFDWCSKQTYIALANMLTGASALG
IDSCPIEGFHYDKMNECLAEEGLFDPQEYAVSVAATFGYRSRDIAKKSRKGLDEVVKWVG

Sequences:

>Translated_220_residues
MTQLTREQVLELFHQRSSTRYYDPTKKISDEDFECILECGRLSPSSVGSEPWKFLVIQNKTLREKMKPFSWGMINQLDNC
SHLVVILAKKNARYDSPFFVDVMARKGLNAEQQQAALTKYKALQEEDMKLLENDRTLFDWCSKQTYIALANMLTGASALG
IDSCPIEGFHYDKMNECLAEEGLFDPQEYAVSVAATFGYRSRDIAKKSRKGLDEVVKWVG
>Mature_219_residues
TQLTREQVLELFHQRSSTRYYDPTKKISDEDFECILECGRLSPSSVGSEPWKFLVIQNKTLREKMKPFSWGMINQLDNCS
HLVVILAKKNARYDSPFFVDVMARKGLNAEQQQAALTKYKALQEEDMKLLENDRTLFDWCSKQTYIALANMLTGASALGI
DSCPIEGFHYDKMNECLAEEGLFDPQEYAVSVAATFGYRSRDIAKKSRKGLDEVVKWVG

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): Y1278_HAEIN (Q57431)

Other databases:

- EMBL:   L42023
- PIR:   B64114
- RefSeq:   NP_439431.1
- ProteinModelPortal:   Q57431
- SMR:   Q57431
- GeneID:   950210
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1278
- NMPDR:   fig|71421.1.peg.1220
- TIGR:   HI_1278
- HOGENOM:   HBG751302
- OMA:   MMTSAAQ
- ProtClustDB:   CLSK870224
- BioCyc:   HINF71421:HI_1278-MONOMER
- InterPro:   IPR000415

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25189; Mature: 25058

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLTREQVLELFHQRSSTRYYDPTKKISDEDFECILECGRLSPSSVGSEPWKFLVIQNK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCH
TLREKMKPFSWGMINQLDNCSHLVVILAKKNARYDSPFFVDVMARKGLNAEQQQAALTKY
HHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
KALQEEDMKLLENDRTLFDWCSKQTYIALANMLTGASALGIDSCPIEGFHYDKMNECLAE
HHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHH
EGLFDPQEYAVSVAATFGYRSRDIAKKSRKGLDEVVKWVG
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
TQLTREQVLELFHQRSSTRYYDPTKKISDEDFECILECGRLSPSSVGSEPWKFLVIQNK
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCH
TLREKMKPFSWGMINQLDNCSHLVVILAKKNARYDSPFFVDVMARKGLNAEQQQAALTKY
HHHHHCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
KALQEEDMKLLENDRTLFDWCSKQTYIALANMLTGASALGIDSCPIEGFHYDKMNECLAE
HHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHH
EGLFDPQEYAVSVAATFGYRSRDIAKKSRKGLDEVVKWVG
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023