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The map label for this gene is dksA

Identifier: 16272036

GI number: 16272036

Start: 65821

End: 66258

Strand: Direct

Name: dksA

Synonym: HI0062

Alternate gene names: 16272036

Gene position: 65821-66258 (Clockwise)

Preceding gene: 16272029

Following gene: 16272037

Centisome position: 3.6

GC content: 40.41

Gene sequence:

>438_bases
ATGTCAAGAGCATCATTGAGCTTGCTAGATTTAGCAGGCGTTAAGCCTTATCAAATGAAAAAAGATGAAGAATATATGAA
TGAAGAGCAAATTCTTCATTTCAGAAAAATTCTTAATGCTTGGCACGAGCAAATCGTGGAAGAAGCTTCTCGCACCGTTG
CACATATGCAGGATGAAGTAACCAATTTCCCCGATCCTGCAGACCGAGCAACTCAAGAAGAGGAATTTAGTCTAGAATTA
CGTAATCGAGATCGCGAACGTAAACTAATGAAAAAAATCGAAGCAACCTTGAAGAAACTTGATACCGATGATTTTGGTTA
CTGCGATTGTTGTGGTGAAGAAATCGGCATTCGCCGTTTAGAAGCGCGCCCAACAGCAGATCTTTGCATTGATTGCAAAA
CTTTAGCTGAAATCCGCGAAAAACAAGTCGCTGGTTAA

Upstream 100 bases:

>100_bases
AAAAATTGGGTTCCTAAAATAGCAGTCAGGAACAAACTAAAATAACAAGACAGGAAGTCGTGAAATCCACGCTTAATTCT
GTATGTATTAGGAGTGAGAG

Downstream 100 bases:

>100_bases
TTCATTCTATTGAAAAATACAAAAGTGCGGTTAAAATTAACCGCACTTTTTTGCATTTAAAAATGCAGGAAAATCCACTT
GCCTTTTTCTTTACAAGAAA

Product: dnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MSRASLSLLDLAGVKPYQMKKDEEYMNEEQILHFRKILNAWHEQIVEEASRTVAHMQDEVTNFPDPADRATQEEEFSLEL
RNRDRERKLMKKIEATLKKLDTDDFGYCDCCGEEIGIRRLEARPTADLCIDCKTLAEIREKQVAG

Sequences:

>Translated_145_residues
MSRASLSLLDLAGVKPYQMKKDEEYMNEEQILHFRKILNAWHEQIVEEASRTVAHMQDEVTNFPDPADRATQEEEFSLEL
RNRDRERKLMKKIEATLKKLDTDDFGYCDCCGEEIGIRRLEARPTADLCIDCKTLAEIREKQVAG
>Mature_144_residues
SRASLSLLDLAGVKPYQMKKDEEYMNEEQILHFRKILNAWHEQIVEEASRTVAHMQDEVTNFPDPADRATQEEEFSLELR
NRDRERKLMKKIEATLKKLDTDDFGYCDCCGEEIGIRRLEARPTADLCIDCKTLAEIREKQVAG

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger

Homologues:

Organism=Escherichia coli, GI1786338, Length=142, Percent_Identity=75.3521126760563, Blast_Score=227, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DKSA_HAEIN (P43758)

Other databases:

- EMBL:   L42023
- EMBL:   L20805
- PIR:   A64046
- RefSeq:   NP_438235.1
- ProteinModelPortal:   P43758
- SMR:   P43758
- GeneID:   950956
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0062
- TIGR:   HI_0062
- HOGENOM:   HBG728818
- OMA:   VDCKTLA
- ProtClustDB:   CLSK808376
- BioCyc:   HINF71421:HI_0062-MONOMER
- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458
- TIGRFAMs:   TIGR02420

Pfam domain/function: PF01258 zf-dskA_traR

EC number: NA

Molecular weight: Translated: 16864; Mature: 16733

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01102 ZF_DKSA_1; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRASLSLLDLAGVKPYQMKKDEEYMNEEQILHFRKILNAWHEQIVEEASRTVAHMQDEV
CCCCHHHHHHHCCCCCHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNFPDPADRATQEEEFSLELRNRDRERKLMKKIEATLKKLDTDDFGYCDCCGEEIGIRRL
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EARPTADLCIDCKTLAEIREKQVAG
CCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRASLSLLDLAGVKPYQMKKDEEYMNEEQILHFRKILNAWHEQIVEEASRTVAHMQDEV
CCCHHHHHHHCCCCCHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNFPDPADRATQEEEFSLELRNRDRERKLMKKIEATLKKLDTDDFGYCDCCGEEIGIRRL
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EARPTADLCIDCKTLAEIREKQVAG
CCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8063112