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The map label for this gene is pcnB

Identifier: 16272037

GI number: 16272037

Start: 66506

End: 67864

Strand: Direct

Name: pcnB

Synonym: HI0063

Alternate gene names: 16272037

Gene position: 66506-67864 (Clockwise)

Preceding gene: 16272036

Following gene: 16272038

Centisome position: 3.63

GC content: 41.35

Gene sequence:

>1359_bases
ATGCCAAAAGCGCGAGCAAAAAAATCAGAACAAACACGTCGCTATGATAAAAATGTGATTAAAGCGGCTCAATTTGATAT
TTCTCCGCGTGATTTTAGCCGTAATGCTCTCAATGTAGTGGAAAAACTGCAACGTCAAGGTTTTGAGGCTTATATCGTCG
GTGGATGTATCCGCGATTTATTGCTCGGTAAAAAACCAAAAGATTTCGATGTTGCAACAAACGCCCGCCCTGAGCAAATC
CAAAACATTTTCCAACGCCAATGCCGTTTAGTGGGTCGCCGTTTCCGCTTAGCACATATTATGTTTGGTCGAGATATTAT
TGAAGTCGCCACTTTCCGTGCTAATCACAGTGATGCTCGCAATGAAAATCAAGCGAAACAGAGCAATGAAGGCATGTTAC
TGCGCGATAATGTTTACGGTACGATCGAACAAGATGCAGCACGCCGTGATTTTACGGTCAATGCACTGTATTACAATCCG
CAAGATAATACGTTACGAGATTATTTCGAGGGCATTAAAGATCTCAAAGCAGGCAAGCTACGCTTAATTGGCGATCCTGT
TACACGTTATCAAGAAGATCCTGTGCGCATGCTACGCTCAATTCGTTTTATGGCAAAACTTGATATGTTCCTTGAAAAAC
CGAGCGAACAACCAATTCGTGAACTTGCGCCTTTATTAAAAAATATTCCGCCAGCTCGCTTATTTGACGAAAGCTTAAAA
TTATTGCAGGCGGGACAAGGGGTAAAAACCTATCGGTTATTACGCCAATATGGTTTATTTGAACAACTTTTCCCTGCATT
AAGCGCATATTTTACAGAGAAAGAAGATAGCTTTGCGGAACGTATGATCGTCACTGCACTTACTTCTACGGATGAACGTG
TTGCGGATAAACTTCGTATTAATCCTGCATTCTTATTTGCAGCATTTTTCTGGTATCCATTGCGCGAAAAAGTGGAAATT
TTAAAAAATGAAGGTGGTTTAAATAATCATGATGCTTATGCTTTAGCAGGCAATGAAGTTTTAGACTTATTCTGCCGAGC
TTTAGCTGCACCACGTCGTCATACTGCGGTCATTCGTGACATTTGGTTCTTACAACTGCAATTATTAAAACGTACTGGAT
CAGCACCAATGCGTACCATGGAACATCCAAAATTCCGTGCAGGCTTTGATTTATTGGCTATGCGAGCAGAAATTGAAGGT
GGCGAAACCATTGAATTAGCCAAATGGTGGCACGAATATCAATTCAGCAATGGTGAACAGCGTGAACAGTTGATACAAGA
GCAACAACGCTTACATCCTAAACCAAAGAAAAAATACTACCGTCCACGCCGTCGTAAAACAACGTGCTCAGCAGAATAA

Upstream 100 bases:

>100_bases
TACTTATATCAAAAATTTGTTTGGCAAGAAGACAAAAAAATCAAAAGAGGTTGCCAAAACAAGTGCTCATTCTAAACGAG
TTGAGCGTTCTAATAGCCAA

Downstream 100 bases:

>100_bases
GGGAAAAGATGATTACGGCATATATTGCTCTAGGTAGCAATTTAAACACACCAGTAGAACAATTACATGCTGCGCTTAAA
GCGATAAGTCAGTTATCAAA

Product: polyA polymerase

Products: NA

Alternate protein names: PAP

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQI
QNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNP
QDNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
LLQAGQGVKTYRLLRQYGLFEQLFPALSAYFTEKEDSFAERMIVTALTSTDERVADKLRINPAFLFAAFFWYPLREKVEI
LKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEG
GETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTCSAE

Sequences:

>Translated_452_residues
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQI
QNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNP
QDNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
LLQAGQGVKTYRLLRQYGLFEQLFPALSAYFTEKEDSFAERMIVTALTSTDERVADKLRINPAFLFAAFFWYPLREKVEI
LKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEG
GETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTCSAE
>Mature_451_residues
PKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQIQ
NIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQ
DNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKL
LQAGQGVKTYRLLRQYGLFEQLFPALSAYFTEKEDSFAERMIVTALTSTDERVADKLRINPAFLFAAFFWYPLREKVEIL
KNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGG
ETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTCSAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family

Homologues:

Organism=Homo sapiens, GI155030240, Length=222, Percent_Identity=31.5315315315315, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=412, Percent_Identity=56.3106796116505, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789436, Length=227, Percent_Identity=28.6343612334802, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=324, Percent_Identity=27.1604938271605, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360112, Length=252, Percent_Identity=27.3809523809524, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357337, Length=252, Percent_Identity=27.3809523809524, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCNB_HAEIN (P44439)

Other databases:

- EMBL:   L42023
- PIR:   B64046
- RefSeq:   NP_438236.1
- ProteinModelPortal:   P44439
- SMR:   P44439
- GeneID:   950961
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0063
- NMPDR:   fig|71421.1.peg.62
- TIGR:   HI_0063
- HOGENOM:   HBG687021
- OMA:   WYPLREK
- ProtClustDB:   CLSK870278
- BioCyc:   HINF71421:HI_0063-MONOMER
- BRENDA:   2.7.7.19
- GO:   GO:0006350
- InterPro:   IPR002646
- InterPro:   IPR010206
- TIGRFAMs:   TIGR01942

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.19

Molecular weight: Translated: 52698; Mature: 52567

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: ACT_SITE 68-68 ACT_SITE 70-70 ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
LLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQDNTLRDYFEGIKDLKAGKL
CHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCE
RLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LLQAGQGVKTYRLLRQYGLFEQLFPALSAYFTEKEDSFAERMIVTALTSTDERVADKLRI
HHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
NPAFLFAAFFWYPLREKVEILKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHH
IWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGGETIELAKWWHEYQFSNGEQ
HHHHHHHHHHHCCCCCHHHHCCCCHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHH
REQLIQEQQRLHPKPKKKYYRPRRRKTTCSAE
HHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
LLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQDNTLRDYFEGIKDLKAGKL
CHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCE
RLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LLQAGQGVKTYRLLRQYGLFEQLFPALSAYFTEKEDSFAERMIVTALTSTDERVADKLRI
HHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
NPAFLFAAFFWYPLREKVEILKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHH
IWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGGETIELAKWWHEYQFSNGEQ
HHHHHHHHHHHCCCCCHHHHCCCCHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHH
REQLIQEQQRLHPKPKKKYYRPRRRKTTCSAE
HHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800