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The map label for this gene is thyA [H]

Identifier: 162139863

GI number: 162139863

Start: 2256598

End: 2257392

Strand: Direct

Name: thyA [H]

Synonym: Nmul_A1962

Alternate gene names: 162139863

Gene position: 2256598-2257392 (Clockwise)

Preceding gene: 82703080

Following gene: 82703084

Centisome position: 70.87

GC content: 52.83

Gene sequence:

>795_bases
ATGCGCCAGTACCTCGATCTCATGCAGCATGTTCTGGAGCACGGACAAAAAAAATCCGATCGCACCGGCACAGGCACTCT
CTCCGTTTTCGGCTACCAGATGCGCTTCGACCTGTCTGAAGGTTTTCCCCTGCTGACGACAAAGAAACTGCATCTGAAAT
CCATCATTTACGAACTGCTCTGGTTTTTGAAAGGGGATACCAATATAAGGTATCTCAACGAAAATGACGTTTCCATCTGG
GATGAATGGGCGGATGAGAATGGCGATCTGGGACCGATATATGGCCGCCAGTGGCGCTCCTGGCCGAACGGGAAGGGAGG
CCATATCGATCAGATCGAACAGGTAGTCGAGCAGATCAGGAACACGCCCGATTCCCGGCGCATGCTGGTATCGTCCTGGA
ATGTGGGGGAACTGCAGGAAATGAAGCTGGCGCCGTGCCACGCGCTGTTTCAGTTCTATGTGGCGGAGGACAAGCTTTCC
TGCCAGCTTTACCAGCGCAGCGCTGACATCTTCCTGGGCGTTCCGTTCAATATTGCCTCCTATGCTCTGCTTACGCTCAT
GCTGGCGCAAAGCTGTGATTTACGCGCGGGTGAATTCGTCCATACTTTTGGCGATGCGCATCTTTATCTCAACCATCTGG
AACAGGCACGCGAGCAATTGGAGCGGGGGCCGAAAAAACTGCCTATCATGAAATTGAACCCGGCAATCCGGAACGTTTTC
GACTTCCGGTATGAGGATTTCAACCTGGAAGGGTACGACCCTCATCCGTCAATCAAGGCACCCATTGCAGTATGA

Upstream 100 bases:

>100_bases
AGCGCTGCAAGCATGGAATCACCTTTATTCTGTTAGAATGTCCATTATACTCGGGGCGTTTCCTTTTACTAAATTCCGCA
GCCCGACAATACCGACTTCG

Downstream 100 bases:

>100_bases
CACCTCGACTCTCCGTTTTGGTGGCGATGGCGCGAAATCGCGCCATCGGTCATAAGAACGCGATCCCCTGGCACTTGCCG
CACGACCTCAAGCATTTCAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRQYLDLMQHVLEHGQKKSDRTGTGTLSVFGYQMRFDLSEGFPLLTTKKLHLKSIIYELLWFLKGDTNIRYLNENDVSIW
DEWADENGDLGPIYGRQWRSWPNGKGGHIDQIEQVVEQIRNTPDSRRMLVSSWNVGELQEMKLAPCHALFQFYVAEDKLS
CQLYQRSADIFLGVPFNIASYALLTLMLAQSCDLRAGEFVHTFGDAHLYLNHLEQAREQLERGPKKLPIMKLNPAIRNVF
DFRYEDFNLEGYDPHPSIKAPIAV

Sequences:

>Translated_264_residues
MRQYLDLMQHVLEHGQKKSDRTGTGTLSVFGYQMRFDLSEGFPLLTTKKLHLKSIIYELLWFLKGDTNIRYLNENDVSIW
DEWADENGDLGPIYGRQWRSWPNGKGGHIDQIEQVVEQIRNTPDSRRMLVSSWNVGELQEMKLAPCHALFQFYVAEDKLS
CQLYQRSADIFLGVPFNIASYALLTLMLAQSCDLRAGEFVHTFGDAHLYLNHLEQAREQLERGPKKLPIMKLNPAIRNVF
DFRYEDFNLEGYDPHPSIKAPIAV
>Mature_264_residues
MRQYLDLMQHVLEHGQKKSDRTGTGTLSVFGYQMRFDLSEGFPLLTTKKLHLKSIIYELLWFLKGDTNIRYLNENDVSIW
DEWADENGDLGPIYGRQWRSWPNGKGGHIDQIEQVVEQIRNTPDSRRMLVSSWNVGELQEMKLAPCHALFQFYVAEDKLS
CQLYQRSADIFLGVPFNIASYALLTLMLAQSCDLRAGEFVHTFGDAHLYLNHLEQAREQLERGPKKLPIMKLNPAIRNVF
DFRYEDFNLEGYDPHPSIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=49.290780141844, Blast_Score=287, Evalue=5e-78,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=67.0454545454545, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=43.4628975265018, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=291, Percent_Identity=36.426116838488, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17137556, Length=285, Percent_Identity=49.4736842105263, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30582; Mature: 30582

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQYLDLMQHVLEHGQKKSDRTGTGTLSVFGYQMRFDLSEGFPLLTTKKLHLKSIIYELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFLKGDTNIRYLNENDVSIWDEWADENGDLGPIYGRQWRSWPNGKGGHIDQIEQVVEQIR
HHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
NTPDSRRMLVSSWNVGELQEMKLAPCHALFQFYVAEDKLSCQLYQRSADIFLGVPFNIAS
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCHHHHH
YALLTLMLAQSCDLRAGEFVHTFGDAHLYLNHLEQAREQLERGPKKLPIMKLNPAIRNVF
HHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHH
DFRYEDFNLEGYDPHPSIKAPIAV
HCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRQYLDLMQHVLEHGQKKSDRTGTGTLSVFGYQMRFDLSEGFPLLTTKKLHLKSIIYELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFLKGDTNIRYLNENDVSIWDEWADENGDLGPIYGRQWRSWPNGKGGHIDQIEQVVEQIR
HHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
NTPDSRRMLVSSWNVGELQEMKLAPCHALFQFYVAEDKLSCQLYQRSADIFLGVPFNIAS
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCHHHHH
YALLTLMLAQSCDLRAGEFVHTFGDAHLYLNHLEQAREQLERGPKKLPIMKLNPAIRNVF
HHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHH
DFRYEDFNLEGYDPHPSIKAPIAV
HCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]