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The map label for this gene is dfrA [H]

Identifier: 82703084

GI number: 82703084

Start: 2257389

End: 2257940

Strand: Direct

Name: dfrA [H]

Synonym: Nmul_A1963

Alternate gene names: 82703084

Gene position: 2257389-2257940 (Clockwise)

Preceding gene: 162139863

Following gene: 82703088

Centisome position: 70.89

GC content: 54.17

Gene sequence:

>552_bases
ATGACACCTCGACTCTCCGTTTTGGTGGCGATGGCGCGAAATCGCGCCATCGGTCATAAGAACGCGATCCCCTGGCACTT
GCCGCACGACCTCAAGCATTTCAAGGAGCTTACGATGGGGCATCCCATGATCATGGGACGCAAGACGTATGAATCCATCG
GCCGGCTTCTTCCCGGCCGCACCAGCATCATTATCACAAGACAACCTGATTATGAAGTTCCGGGCGCACTGGTGGTCAGC
TCCATAGGAGAAGCGCTCAGCGCCTGCAAGCAGATAAGAAGCGGAGAAGAGGGGCATCCCGATCAAGGGAACGAATGTTT
CGTCATTGGGGGTGCAGATATATTCCAGCAAATGCTGCCGCTATGTGATCGCCTGTATATGACCGAGATCCACAGGGATT
TCGAGGGTGATACGTATTTTCCGGAATTTGACAGGAATGATTGGTGGGAGGTCTCGCGCGAAGAGCATGTGGATGACAGT
GGGCTGGAGTACCATTTTGTCACACTCGATCGCATGGCGAGTCAGCAGGAACAAGGCGCTCAGCGACTCTGA

Upstream 100 bases:

>100_bases
AATTGAACCCGGCAATCCGGAACGTTTTCGACTTCCGGTATGAGGATTTCAACCTGGAAGGGTACGACCCTCATCCGTCA
ATCAAGGCACCCATTGCAGT

Downstream 100 bases:

>100_bases
GTTCCGGGCTCTGATTCCCGACAAGCGTTCCTGCAAAAAAAGTGGCGGGCCACAGATAACTGTGACCCGCCTTTTTTATT
ATTATTGTCCGATTGCGCTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MTPRLSVLVAMARNRAIGHKNAIPWHLPHDLKHFKELTMGHPMIMGRKTYESIGRLLPGRTSIIITRQPDYEVPGALVVS
SIGEALSACKQIRSGEEGHPDQGNECFVIGGADIFQQMLPLCDRLYMTEIHRDFEGDTYFPEFDRNDWWEVSREEHVDDS
GLEYHFVTLDRMASQQEQGAQRL

Sequences:

>Translated_183_residues
MTPRLSVLVAMARNRAIGHKNAIPWHLPHDLKHFKELTMGHPMIMGRKTYESIGRLLPGRTSIIITRQPDYEVPGALVVS
SIGEALSACKQIRSGEEGHPDQGNECFVIGGADIFQQMLPLCDRLYMTEIHRDFEGDTYFPEFDRNDWWEVSREEHVDDS
GLEYHFVTLDRMASQQEQGAQRL
>Mature_182_residues
TPRLSVLVAMARNRAIGHKNAIPWHLPHDLKHFKELTMGHPMIMGRKTYESIGRLLPGRTSIIITRQPDYEVPGALVVSS
IGEALSACKQIRSGEEGHPDQGNECFVIGGADIFQQMLPLCDRLYMTEIHRDFEGDTYFPEFDRNDWWEVSREEHVDDSG
LEYHFVTLDRMASQQEQGAQRL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=193, Percent_Identity=26.9430051813472, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1786233, Length=169, Percent_Identity=41.4201183431953, Blast_Score=130, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=28.9617486338798, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=141, Percent_Identity=28.3687943262411, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008026, Length=187, Percent_Identity=25.1336898395722, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24647458, Length=187, Percent_Identity=25.1336898395722, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20936; Mature: 20805

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRLSVLVAMARNRAIGHKNAIPWHLPHDLKHFKELTMGHPMIMGRKTYESIGRLLPGR
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCC
TSIIITRQPDYEVPGALVVSSIGEALSACKQIRSGEEGHPDQGNECFVIGGADIFQQMLP
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHH
LCDRLYMTEIHRDFEGDTYFPEFDRNDWWEVSREEHVDDSGLEYHFVTLDRMASQQEQGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHH
QRL
CCC
>Mature Secondary Structure 
TPRLSVLVAMARNRAIGHKNAIPWHLPHDLKHFKELTMGHPMIMGRKTYESIGRLLPGR
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCC
TSIIITRQPDYEVPGALVVSSIGEALSACKQIRSGEEGHPDQGNECFVIGGADIFQQMLP
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHH
LCDRLYMTEIHRDFEGDTYFPEFDRNDWWEVSREEHVDDSGLEYHFVTLDRMASQQEQGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHH
QRL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]