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The map label for this gene is trmL [H]

Identifier: 161870795

GI number: 161870795

Start: 1928084

End: 1928557

Strand: Direct

Name: trmL [H]

Synonym: NMCC_1877

Alternate gene names: 161870795

Gene position: 1928084-1928557 (Clockwise)

Preceding gene: 161870794

Following gene: 161870796

Centisome position: 89.54

GC content: 58.02

Gene sequence:

>474_bases
TTGATATTTATGTTTACCATCGTTTTATACCAACCGGAAATCCCCCCGAACACGGGCAACATCATCCGCCTGTGCGCCAA
TACCGGCGCGGATTTGCACCTTGTCAAACCGCTCGGCTTCCCATTGGATTCCGCCAAAATGAAACGCGCCGGGCTCGACT
ACCACGAATTCGCCAGCCTGACGGTGTACGAAAACTTCGACGACTGCCTCAAGGCACTCGCAGGCAGGCGCATTTTCGCC
CTGACCACCAAAGGCACGGCGCGCCCCGATGAAACCGCGTTTCAAAAAGGCGACGTTTTACTGTTCGGGCCGGAAACGCG
CGGACTGCCTGCCGACATCCTCGACAGCCTGCCCGCCGCGCAAAAAATCCGCCTGCCCATGCAGCCCGGCAGCCGGAGTA
TGAACCTTTCCAATACCGTTGCCGTGATTCTCTTTGAAGCGTGGCGGCAACACGGTTACGCAGGCGGCGTTTGA

Upstream 100 bases:

>100_bases
CGAATCTGCTGCTGGACGCTGGCACGCACGCCAATGAAAAAATAACTAAATTTTTTGACACCCCATCCGCCTTGTGGCAA
GGTTACGCGCCTATAAGTGA

Downstream 100 bases:

>100_bases
ACGCAAGTTCATGCCGTCTGAAAACCTATCCGGACACATTCCGAACCGCCGTCCGCACTGTACGGCGGTTCGGAACGTAT
CCGGCGGGCATGATGCCCAT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MIFMFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKMKRAGLDYHEFASLTVYENFDDCLKALAGRRIFA
LTTKGTARPDETAFQKGDVLLFGPETRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV

Sequences:

>Translated_157_residues
MIFMFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKMKRAGLDYHEFASLTVYENFDDCLKALAGRRIFA
LTTKGTARPDETAFQKGDVLLFGPETRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV
>Mature_157_residues
MIFMFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKMKRAGLDYHEFASLTVYENFDDCLKALAGRRIFA
LTTKGTARPDETAFQKGDVLLFGPETRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=62.3376623376623, Blast_Score=210, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17237; Mature: 17237

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFMFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKMKRAGLDYHEFASL
CEEEEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCHHHHEEE
TVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPETRGLPADILDSLPAA
EEECCHHHHHHHHHCCEEEEEECCCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCC
QKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFMFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKMKRAGLDYHEFASL
CEEEEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCHHHHEEE
TVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPETRGLPADILDSLPAA
EEECCHHHHHHHHHCCEEEEEECCCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCC
QKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA