Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 161870226

GI number: 161870226

Start: 1265783

End: 1267228

Strand: Direct

Name: gatA [H]

Synonym: NMCC_1267

Alternate gene names: 161870226

Gene position: 1265783-1267228 (Clockwise)

Preceding gene: 161870225

Following gene: 161870227

Centisome position: 58.78

GC content: 57.05

Gene sequence:

>1446_bases
ATGACCCAATACACATTGAAACAGGCAAGCGTCCTGTTGCAGTCCAAACAGATTTCCGCCGTCGAACTGGCAAGCGCATA
CCTTGCCGCCATTGCCGAAAAAAATCCCGCCCTCAACGGCTATATCACCATCGACCAAGATAAAACCCTTGCAGAAGCCC
GCGCCGCCGACGAACGTATCGCTCAGGGCAACGCTTCGGCACTTACCGGCATACCCGTCGCCTACAAGGATATTTTCTGC
CAAACCGGCTGGCGCAGCGCGTGCGCTTCCAAAATGCTCGACAACTTCATCTCCTCCTACACCGCCACCGTCGTCCAAAA
CCTGCTCGACGAAGGTATGGTAACGCTCGGCCGCACCAATATGGATGAGTTCGCTATGGGTTCGACCAATGAAAACTCAT
TCTACGGCGCGACCAAAAACCCGTGGAATCCCGAACACGTCCCCGGCGGTTCGTCCGGCGGTTCGGCAGCCGTCGTTGCC
GCCCGCCTTGCTCCTGCCGCGCTCGGTTCGGACACGGGCGGCTCTATCCGCCAACCCGCATCGCACTGCGGTATTACCGG
TATCAAACCTACCTACGGCACGGTTTCCCGCTTCGGTATGGTCGCCTACGCCTCCAGCTTCGATCAAGCCGGCCCGATGG
CGCAAACTGCCGAAGACTGCGCGATTCTGTTAAACGCGATGGCAGGTTTCGACCCCAAAGACTCCACCAGCCTCGAGCGC
GAAAAAGAAGACTACACCCGCGATTTGAACCAACCGCTCAAAGGTTTGAAAATCGGCCTGCCCAAAGAATATTTCGGCGA
AGGTAACAGCGCCGATGTTCTGACGGCATTGCAAAACACCATTGATTTGCTGAAAGCCCAAGGCGCGGAATTGATTGAAG
TTTCCCTGCCGCAAACCAAGCTGTCCATCCCCGCCTACTACGTCCTCGCCTCCGCAGAAGCCAGCACCAACCTTTCACGT
TACGACGGCGTACGTTACGGACACCGTGCCGCCCAATTCGGCGATTTGGAAGAAATGTACGGCAAAACCCGCGCCGAAGG
TTTCGGCAGCGAAGTCAAACGCCGCATCATGATCGGCACTTATGTACTGTCGCACGGCTACTACGATGCCTACTATCTCA
AAGCCCAAAAACTGCGCCGCCTCGTTGCCGATGATTTTCAGACGGTATTTGCACGGTGCGACCTCATCCTCGCGCCGACC
GCACCCACTGCAGCCCCAAAAATCGGAGCGGATTCTTCGCCGGTTGAAACCTACTTGAGCGATATCTACACCATCGCCGT
CAACCTCGCCGGACTACCCGCATTGACCCTGCCTGCAGGTTTCAGCGGCGGCGGACTGCCCGTCGGTGTTCAGCTTATCG
GCAACTACTTCGCCGAAGCCAGAATACTCGGTGCGGCGCATCAAATCCAACTCAACAGCGATTGGCACGGCAAACGACCC
GAATGA

Upstream 100 bases:

>100_bases
ACCGTCTGTACATCGTACCGCAAGTTATCGAAGAATAATCCGAATATGCTTCAGACGGCATCAGCAATACCGCCCGAAGC
CCTTTAAGGATGGAAGATTT

Downstream 100 bases:

>100_bases
AGCAGAACCGCACCTTTACCTTCCCCGATTTTCGCACCGTTTACAGCTATGCGCCTTTATATCGGCTGCAACATTTAAAA
TACACATTGCGAAAATTTTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERIAQGNASALTGIPVAYKDIFC
QTGWRSACASKMLDNFISSYTATVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGATKNPWNPEHVPGGSSGGSAAVVA
ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLER
EKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSR
YDGVRYGHRAAQFGDLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTVFARCDLILAPT
APTAAPKIGADSSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLIGNYFAEARILGAAHQIQLNSDWHGKRP
E

Sequences:

>Translated_481_residues
MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERIAQGNASALTGIPVAYKDIFC
QTGWRSACASKMLDNFISSYTATVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGATKNPWNPEHVPGGSSGGSAAVVA
ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLER
EKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSR
YDGVRYGHRAAQFGDLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTVFARCDLILAPT
APTAAPKIGADSSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLIGNYFAEARILGAAHQIQLNSDWHGKRP
E
>Mature_480_residues
TQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERIAQGNASALTGIPVAYKDIFCQ
TGWRSACASKMLDNFISSYTATVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGATKNPWNPEHVPGGSSGGSAAVVAA
RLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLERE
KEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRY
DGVRYGHRAAQFGDLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTVFARCDLILAPTA
PTAAPKIGADSSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLIGNYFAEARILGAAHQIQLNSDWHGKRPE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=38.3697813121272, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=26.7206477732794, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=415, Percent_Identity=39.7590361445783, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=23.6252545824847, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=33.6898395721925, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=241, Percent_Identity=30.7053941908714, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=180, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=180, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=182, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=435, Percent_Identity=35.8620689655172, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=492, Percent_Identity=28.4552845528455, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=40.2040816326531, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=26.7489711934156, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=26.7489711934156, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=26.7489711934156, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=26.7489711934156, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=24.1516966067864, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078093, Length=475, Percent_Identity=27.7894736842105, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=33.1914893617021, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=33.1914893617021, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=33.1914893617021, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=23.9837398373984, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=35.2601156069364, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=35.2601156069364, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51340; Mature: 51209

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERI
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHH
AQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISSYTATVVQNLLDEGMVTLGRTN
HCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
MDEFAMGSTNENSFYGATKNPWNPEHVPGGSSGGSAAVVAARLAPAALGSDTGGSIRQPA
HHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
SHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLER
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
EKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQTK
HHHHHHHHHHHCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFGDLEEMYGKTRAEGFGSEVKRRIMIGT
ECCCEEEEEEECHHHCCHHHHCCCHHCCHHHHCCCHHHHHCHHHHHCCHHHHHHHHHHHH
YVLSHGYYDAYYLKAQKLRRLVADDFQTVFARCDLILAPTAPTAAPKIGADSSPVETYLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHH
DIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLIGNYFAEARILGAAHQIQLNSDWHGKRP
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
E
C
>Mature Secondary Structure 
TQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERI
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHH
AQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISSYTATVVQNLLDEGMVTLGRTN
HCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
MDEFAMGSTNENSFYGATKNPWNPEHVPGGSSGGSAAVVAARLAPAALGSDTGGSIRQPA
HHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
SHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLER
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
EKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQTK
HHHHHHHHHHHCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFGDLEEMYGKTRAEGFGSEVKRRIMIGT
ECCCEEEEEEECHHHCCHHHHCCCHHCCHHHHCCCHHHHHCHHHHHCCHHHHHHHHHHHH
YVLSHGYYDAYYLKAQKLRRLVADDFQTVFARCDLILAPTAPTAAPKIGADSSPVETYLS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHH
DIYTIAVNLAGLPALTLPAGFSGGGLPVGVQLIGNYFAEARILGAAHQIQLNSDWHGKRP
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA