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The map label for this gene is nlaIVR [H]

Identifier: 161870124

GI number: 161870124

Start: 1153900

End: 1154631

Strand: Direct

Name: nlaIVR [H]

Synonym: NMCC_1163

Alternate gene names: 161870124

Gene position: 1153900-1154631 (Clockwise)

Preceding gene: 161870123

Following gene: 161870125

Centisome position: 53.58

GC content: 37.43

Gene sequence:

>732_bases
ATGGTAAAACTTACTGCACAACAAATATTTGATAAGTTGCTGAATGAGGAAAGAATCTTATCGGCTAATGGTCAAATTAG
ATTTTTCTTGGGCGATGTGGATATTATCGTCAAACAAAAAGATGTTGTCGGCAACATCATTCAGGAATGGCTCGGCGGAT
GGTTGAGAAAAAGGGAAATTGAATTTGATGTTTCAACCAATACCCAAATGCCCCCCGATTTCTTTTTAAATAAAAAAGAC
CGCAGTAGAGAATTGTTGGAAGTAAAGGCGTTCAACCGAAATGCCAGCCCGGGTTTTGATATTGCAGATTTTAAAATGTA
TTCTGATGAAATCATTCATAAACCCTATATGCTGGATGTAGATTATTTAATATTCGGTTACGATATGGACGACAACGGCA
ATGTAACCATCAAGGATTTATGGCTTAAAAAAGTATGGCAAATTACCAGAAGTATGGATGGATGGGCAATTAATCTTCAA
GTCAAAAAAGGCGTGGTGCATAAAATCCGCCCGGGTGTTTGGTACAGCATAAATAAAAAGAATATGCCAATGTTTGAATG
CTTGGAAGATTTTGTTTCCGCAATTGAAGAAACCGTTTATCAAAACCCGGCAACACGGCATAACGCATCCTTATGGAAAA
GGAAATTTGAGGAAGCCTATAAAAAACACTACAACCGATCGATTTCCATACCCCGTTGGCACGAAATTGCACATAAATAC
CAAAAGAAATAA

Upstream 100 bases:

>100_bases
AAAAGACAAATACGATTTATTGGGCAATACCGTTGCCGTTCCTGTGATTAAGGCGGTATCTGAAAGGCTTCTGCATACTT
TATAAACCGATAGGGATAAA

Downstream 100 bases:

>100_bases
CCCTTCAACCATCAACCCATAAGGAAACTTAAAATGACCAAACATATCGCCATCCTCCGAGGCGACGGCATCGGCCCTGA
AATCGTCGCCGAAACCGTCC

Product: type II restriction enzyme NlaIV

Products: NA

Alternate protein names: R.NlaIV; Endonuclease NlaIV; Type II restriction enzyme NlaIV [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVKLTAQQIFDKLLNEERILSANGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKD
RSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKRKFEEAYKKHYNRSISIPRWHEIAHKY
QKK

Sequences:

>Translated_243_residues
MVKLTAQQIFDKLLNEERILSANGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKD
RSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKRKFEEAYKKHYNRSISIPRWHEIAHKY
QKK
>Mature_243_residues
MVKLTAQQIFDKLLNEERILSANGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKD
RSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKRKFEEAYKKHYNRSISIPRWHEIAHKY
QKK

Specific function: Recognizes the double-stranded sequence GGNNCC and cleaves after N-3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019064 [H]

Pfam domain/function: PF09564 RE_NgoBV [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 28840; Mature: 28840

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLTAQQIFDKLLNEERILSANGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREI
CCEECHHHHHHHHCCCCEEEECCCEEEEEEECEEEEEECHHHHHHHHHHHHHHHHHHHEE
EFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDV
EEEECCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
DYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQVKKGVVHKIRPGVWYSINKK
EEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHCCCCEEEEECCC
NMPMFECLEDFVSAIEETVYQNPATRHNASLWKRKFEEAYKKHYNRSISIPRWHEIAHKY
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QKK
CCC
>Mature Secondary Structure
MVKLTAQQIFDKLLNEERILSANGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREI
CCEECHHHHHHHHCCCCEEEECCCEEEEEEECEEEEEECHHHHHHHHHHHHHHHHHHHEE
EFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDV
EEEECCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
DYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQVKKGVVHKIRPGVWYSINKK
EEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHCCCCEEEEECCC
NMPMFECLEDFVSAIEETVYQNPATRHNASLWKRKFEEAYKKHYNRSISIPRWHEIAHKY
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8190068 [H]