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The map label for this gene is leuB [H]

Identifier: 161870125

GI number: 161870125

Start: 1154665

End: 1155735

Strand: Direct

Name: leuB [H]

Synonym: NMCC_1164

Alternate gene names: 161870125

Gene position: 1154665-1155735 (Clockwise)

Preceding gene: 161870124

Following gene: 161870126

Centisome position: 53.62

GC content: 52.29

Gene sequence:

>1071_bases
ATGACCAAACATATCGCCATCCTCCGAGGCGACGGCATCGGCCCTGAAATCGTCGCCGAAACCGTCCGCGTACTCGACAA
ACTCATCGCACAAGGCTTGGACGTCGGCTACGAATACGCGCCTTTGGGCGGCGAAGCCTATGACGAATACGGCCATCCTT
ATCCAGAATTTACGCAAAACCTTTGCCGTAAAGCCGATGCCGTATTACTTGGTGCAGTCGGTTCTCCGCAATACGACAAT
CTTGACCGTCCGCTGCGCCCTGAGCGCGGCTTGTTGGCCATCCGTAAAGACCTGAATCTGTTTGCCAATTTGCGTCCTGC
CATTTTGTATAAAGAGCTGGCCAATGCTTCCACTTTGAAACCGGAAATCGTAGCCGGCCTCGACATCCTTATCGTACGCG
AACTCACCGGTGATATTTACTTTGGCGAACCGCGCGGCATCCGTATTTTGGAAAACGGCGAACGCGAAGGCTACAACACC
ATGAAATACAGCGAAAGCGAAATCCGCCGTATTGCCCATGTTGCCTTCCAATCCGCCCAAAAACGCAGCAAAAAAGTCTG
TTCCGTAGGCAAAGCCAACGTTTTGGAAACCACCGAACTGTGGCGCGAAGTCTTTGAAGAAATCGGCAAAGAATACCCTG
ATGTCGAGCTTTCCCATATGTACGTTGATAACGCAGCCATGCAGTTGGTACGCGCGCCTAAACAATTTGACGTGATTGCC
ACCGGCAACATATTCGGCGACATTCTGTCTGACGAAGCCTCCATGCTGACCGGTTCCATCGGTATGCTGCCTTCCGCTTC
TTTGGACGAAAACGGCAAAGGCCTGTACGAACCGTCTCACGGCTCTGCTCCTGACATTGCCGGTCAAAACAAAGCCAATC
CGCTGGCGACCGTATTGTCTCTTGCTATGCTGCTGCGTTACAGCCTGAACGACGAAGCACGCGCGCAACAAGTTGAAAAC
GCTGTACAAAAAGTACTGCAACAAGGCTTGCGTACCAACGACATTTACGAAGAAGGCACAAAACTCGTTTCCTGCTCCGA
AATGGGCGATGCGGTACTCGCCGCCTTGTAA

Upstream 100 bases:

>100_bases
ACACTACAACCGATCGATTTCCATACCCCGTTGGCACGAAATTGCACATAAATACCAAAAGAAATAACCCTTCAACCATC
AACCCATAAGGAAACTTAAA

Downstream 100 bases:

>100_bases
AAAAACAAAGCGCGGATCACACCGCGCTTTTTCATCTGCCCTTTTCCGCTCGCCCCGTTCGTCAACCGCCTGCAAACCCA
CCGTTTACCGGCAGGAAATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDN
LDRPLRPERGLLAIRKDLNLFANLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNT
MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIA
TGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLSLAMLLRYSLNDEARAQQVEN
AVQKVLQQGLRTNDIYEEGTKLVSCSEMGDAVLAAL

Sequences:

>Translated_356_residues
MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDN
LDRPLRPERGLLAIRKDLNLFANLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNT
MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIA
TGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLSLAMLLRYSLNDEARAQQVEN
AVQKVLQQGLRTNDIYEEGTKLVSCSEMGDAVLAAL
>Mature_355_residues
TKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNL
DRPLRPERGLLAIRKDLNLFANLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNTM
KYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIAT
GNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLSLAMLLRYSLNDEARAQQVENA
VQKVLQQGLRTNDIYEEGTKLVSCSEMGDAVLAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=331, Percent_Identity=30.2114803625378, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI28178816, Length=361, Percent_Identity=28.2548476454294, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=29.6511627906977, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI28178821, Length=325, Percent_Identity=28.3076923076923, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=29.8461538461538, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI28178819, Length=160, Percent_Identity=33.125, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=52.5568181818182, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=33.4285714285714, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=26.8844221105528, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=31.2849162011173, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=349, Percent_Identity=30.3724928366762, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=30.0561797752809, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=343, Percent_Identity=27.9883381924198, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=43.3701657458564, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=367, Percent_Identity=33.5149863760218, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=31.7507418397626, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=368, Percent_Identity=31.25, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24643268, Length=367, Percent_Identity=31.0626702997275, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643270, Length=367, Percent_Identity=31.0626702997275, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=369, Percent_Identity=29.2682926829268, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=30.0291545189504, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=30.0291545189504, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078635, Length=250, Percent_Identity=30, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI161078633, Length=250, Percent_Identity=30, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24650122, Length=250, Percent_Identity=30, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI161078639, Length=250, Percent_Identity=30, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI161078637, Length=250, Percent_Identity=30, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130355, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39070; Mature: 38939

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQN
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCCCCHHHHHH
LCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFANLRPAILYKELANASTLK
HHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
PEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNTMKYSESEIRRIAHVAFQSAQ
HHHHHCHHHEEEEECCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCCCCEEEE
TGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLS
CCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LAMLLRYSLNDEARAQQVENAVQKVLQQGLRTNDIYEEGTKLVSCSEMGDAVLAAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHHHHCCHHHHCC
>Mature Secondary Structure 
TKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQN
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCCCCHHHHHH
LCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFANLRPAILYKELANASTLK
HHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
PEIVAGLDILIVRELTGDIYFGEPRGIRILENGEREGYNTMKYSESEIRRIAHVAFQSAQ
HHHHHCHHHEEEEECCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRSKKVCSVGKANVLETTELWREVFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCCCCEEEE
TGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLS
CCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LAMLLRYSLNDEARAQQVENAVQKVLQQGLRTNDIYEEGTKLVSCSEMGDAVLAAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA