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The map label for this gene is ngoBVM [H]

Identifier: 161870123

GI number: 161870123

Start: 1152613

End: 1153884

Strand: Direct

Name: ngoBVM [H]

Synonym: NMCC_1162

Alternate gene names: 161870123

Gene position: 1152613-1153884 (Clockwise)

Preceding gene: 161870122

Following gene: 161870124

Centisome position: 53.52

GC content: 39.94

Gene sequence:

>1272_bases
ATGCAACAGATTAAATTTATTGACTTATTTTCCGGGATGAGCGGTATCAGGAAAGGATTTGAACAAGCCTGTCGGAAACA
GTCGGTTGCTTGCGAATGTGTTTTTACCTCAGAAATCAAACCGGCAGCTTTGGAAGTATTGAAGCAAAACTATCCTGATG
AAGTGCCGTATGGAGATATAACGAAAATTGAAACGGGAGATATTCCCGATTTTGACATCCTGTTGGCAGGCTTCCCTTGC
CAGGCTTTTTCTTTCGCCGGAAAAAGATTGGGCTTTGAAGATACGCGGGGAACGCTTTTCTTTGATGTAGCAAGGATTTT
AAAGGCAAAAAAACCAAAAGGTTTTATTTTGGAAAATGTGGAAGGATTGGTAACGCACGATAGAAAAGATCCGACACAAA
AAATAGGGCGCACCCTGACCGTTATTTTGGAAACCTTGGAAGCATTGGGTTACTATGTTTCTTGGAAAGTTTTAAATGCA
AAAGATTTCGGCATTCCCCAAAACAGGAAGCGCATTTATCTGACAGGCAGTTTGAAATCCAAACCCGACTTATCTTTTGA
AACAACTCTAAGTCCGAAATTAAAAAATATTTTGGAATCGGGACTGCCTACCGAAAGCAGTCCTTTCATCAAAAAATTGC
TAAAAAAATTTCCCCCGTCCGAACTGTACGGAAAATCAGTAAAGGACAAACGGGGAGGGAAAAACAATATCCACAGTTGG
GATATTGAATTAAAAGGCACAGTAACCGAAGAAGAGAAGCAATTATTAAATATGCTTCTAAAAGAACGGAGGAAGAAAAA
ATGGGCTTCAGAAATCGGCATCGATTGGATGGATGGGATGCCTTTGACAAAAGCGCAAATTTCAACTTTCTATAAACACC
CCAATCTTCAAAATATTTTGGACAGCCTGACAGATAAAGGCTATTTGGTTTTGGAGCATCCGAAACAAAAAATTGGCGGG
CGAAGAATCAAAGATGAATCCCTGCCTAAGGGCTACAATATCGTTTCAGGTAAAAAATCATTTGAAATCAATAAAATATT
AGACCCAAATGATGTCGCGCCAACCTTAGTTGCAATGGATATGGAGCACCTTTTCGTCGTTGACAACGGCGGTTTGCGTA
CACTTACCGGAAAAGAAGGTTTACGCTTATTCGGCTATCCGGACGATTATTCGTTTGATATTCCTAAAAAAGACAAATAC
GATTTATTGGGCAATACCGTTGCCGTTCCTGTGATTAAGGCGGTATCTGAAAGGCTTCTGCATACTTTATAA

Upstream 100 bases:

>100_bases
CAGAGGGGCTATAAATTGCTTTCATCTTTCAAGCGGGCTTAGCAACGCTCTAAATTAATGCAATACAGCTCTATTTCAAA
TAAATTTTTAGATTTTTAAT

Downstream 100 bases:

>100_bases
ACCGATAGGGATAAAATGGTAAAACTTACTGCACAACAAATATTTGATAAGTTGCTGAATGAGGAAAGAATCTTATCGGC
TAATGGTCAAATTAGATTTT

Product: DNA (cytosine-5-)-methyltransferase

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAALEVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPC
QAFSFAGKRLGFEDTRGTLFFDVARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSW
DIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDWMDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGG
RRIKDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKY
DLLGNTVAVPVIKAVSERLLHTL

Sequences:

>Translated_423_residues
MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAALEVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPC
QAFSFAGKRLGFEDTRGTLFFDVARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSW
DIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDWMDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGG
RRIKDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKY
DLLGNTVAVPVIKAVSERLLHTL
>Mature_423_residues
MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAALEVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPC
QAFSFAGKRLGFEDTRGTLFFDVARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNA
KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSW
DIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDWMDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGG
RRIKDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKY
DLLGNTVAVPVIKAVSERLLHTL

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=187, Percent_Identity=26.7379679144385, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788271, Length=211, Percent_Identity=39.8104265402844, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47708; Mature: 47708

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAALEVLKQNYPDEVPYGDI
CCCEEEHHHHHCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
TKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFDVARILKAKKPKGFILENV
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHCC
EGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKS
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCEEEEEECCCC
KPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSW
CCCCCEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEE
DIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDWMDGMPLTKAQISTFYKHPNLQNIL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
DSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMD
HHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCHHEEEC
MEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKYDLLGNTVAVPVIKAVSERLL
CCEEEEEECCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHH
HTL
HCC
>Mature Secondary Structure
MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAALEVLKQNYPDEVPYGDI
CCCEEEHHHHHCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
TKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFDVARILKAKKPKGFILENV
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHCC
EGLVTHDRKDPTQKIGRTLTVILETLEALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKS
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCEEEEEECCCC
KPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSW
CCCCCEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEE
DIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDWMDGMPLTKAQISTFYKHPNLQNIL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
DSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMD
HHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCHHEEEC
MEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSFDIPKKDKYDLLGNTVAVPVIKAVSERLL
CCEEEEEECCCEEEECCCCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHH
HTL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]