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The map label for this gene is shiA [H]

Identifier: 161525234

GI number: 161525234

Start: 2257239

End: 2258549

Strand: Reverse

Name: shiA [H]

Synonym: Bmul_2064

Alternate gene names: 161525234

Gene position: 2258549-2257239 (Counterclockwise)

Preceding gene: 161525236

Following gene: 161525233

Centisome position: 65.49

GC content: 68.42

Gene sequence:

>1311_bases
ATGACCCAGTCTCTTCCTTCCGCCCGTCAGCCGGGCCGCGCCGCGACGGCCGCGTTCATCGGCACGATGATCGAGTGGTA
CGACTTCTACATCTATGCGACCGCCGCCGCGCTCGTGTTCGGCGAGCTGTATTTCCCGTCGCACGACCGCTTCGTCAGCA
CGATGGCGTCGTTCGCGACGTTCGCGGTCGGCTTTTTCGCGCGCCCGCTCGGCGGCATCGTGTTCGGCCATCTCGGCGAT
CGCATCGGCCGCAAGAAGGCGCTGATGACGACGCTGATGATGATGGGCGTCGCGACCGTTTGCGTCGGGCTGCTGCCCGA
CTACGCGACGGTCGGCATGCTCGCGCCGGTGCTGCTCGTCGTGCTGCGCATCGTCCAGGGCATCGCGGTCGGCGGCGAGT
GGGGCGGCGCGGTGCTGATGGCGGGCGAGCATGCGCCGCAGGGCCGCCGCACGTTCTTCGCGTCGTTCGCGCAGCTCGGT
AGCCCGGCCGGCCTGATTCTGTCGCTGCTCGCGTTTCGCGCGGTCACGTCGATGGACAAGGCCGACTTCCTCGCATGGGG
CTGGCGCCTGCCGTTTCTCGCGAGCGCGGTGCTGCTGATCGTCGGCATCCTGATCCGGCTCGGCGTGAACGAGTCGCCCG
AATTCGCGCGCGTGAAGGAAGCGAACCGCACGGTGAAGCTGCCGGTCGCCGAAGTATTCCGCTCCGCCTGGGGGCTCGTC
CTGCTCTGCATCGGCGCGAACACGATCGGGATCGCGGGCGTCTATTTCACGAACACGTTCATGATCGCGTACACCACGCA
GTACGTCGGCGTGTCGCGGTCGCTGATTCTCGACAGCCTGTTCGCGGTCGCGATCATCCAGTTCGTCGGCCAGCCGATTT
CCGCGTGGATCGCCGAGCGCATCGGCGGCGCGCGCTTTCTGAAGCTCGCCGCCGCGCTCGCGATGCTGTCGCCGTATCCG
ATGTTCATGCTCGTGCAGGGCGGCACGTCGGCGTCGCTCGTGACGGGCATCGCGATCGCGGTCGTCTGCATGTCGGGCTT
CTATTCGGTGATCGCGGGCTTCGTCAGCGGCGTGTTTCCCGCGCACGTGCGCTATTCGGCGATCTCGCTCGCGTACCAGA
TCTGCGGCGCACTCGCGGGCGGCCTGACGCCGCTCGTCGGCACGTGGCTCGCGCACCGCTTCGCGGGCCAGTGGTGGCCG
CTCGCGGTGTTCTACACGTGCCTGTCCGGCATCTCGCTGCTCTGCATCGTCGCGCTCGATGCGCGCCGCGCGGCGCAGCC
GGCCGCCGCCGAAGCGCTCGGCACGCATTGA

Upstream 100 bases:

>100_bases
GAGGCGTGCCGCAGCGTGCGGCGCGAGGCCGCGACAGACGGTCGCCATCACCATATTGCGTGCCGTTGCCGAGCGGCCCG
GCGCGCGACAGGAGCCCCGC

Downstream 100 bases:

>100_bases
GCATCGACCCACGGTTTTATCGGGAGTGCATATGAAAGATCTGTTGGAAATCGACGGCGAGCGCCTGTGGCGTAGCCTGA
CGGACATGGCGCGCATCGGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTQSLPSARQPGRAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGIVFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPDYATVGMLAPVLLVVLRIVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLLAFRAVTSMDKADFLAWGWRLPFLASAVLLIVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSAWGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFVGQPISAWIAERIGGARFLKLAAALAMLSPYP
MFMLVQGGTSASLVTGIAIAVVCMSGFYSVIAGFVSGVFPAHVRYSAISLAYQICGALAGGLTPLVGTWLAHRFAGQWWP
LAVFYTCLSGISLLCIVALDARRAAQPAAAEALGTH

Sequences:

>Translated_436_residues
MTQSLPSARQPGRAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGIVFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPDYATVGMLAPVLLVVLRIVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLLAFRAVTSMDKADFLAWGWRLPFLASAVLLIVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSAWGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFVGQPISAWIAERIGGARFLKLAAALAMLSPYP
MFMLVQGGTSASLVTGIAIAVVCMSGFYSVIAGFVSGVFPAHVRYSAISLAYQICGALAGGLTPLVGTWLAHRFAGQWWP
LAVFYTCLSGISLLCIVALDARRAAQPAAAEALGTH
>Mature_435_residues
TQSLPSARQPGRAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFATFAVGFFARPLGGIVFGHLGDR
IGRKKALMTTLMMMGVATVCVGLLPDYATVGMLAPVLLVVLRIVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGS
PAGLILSLLAFRAVTSMDKADFLAWGWRLPFLASAVLLIVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSAWGLVL
LCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFVGQPISAWIAERIGGARFLKLAAALAMLSPYPM
FMLVQGGTSASLVTGIAIAVVCMSGFYSVIAGFVSGVFPAHVRYSAISLAYQICGALAGGLTPLVGTWLAHRFAGQWWPL
AVFYTCLSGISLLCIVALDARRAAQPAAAEALGTH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=38.2830626450116, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=31.7972350230415, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=33.6448598130841, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788942, Length=366, Percent_Identity=29.5081967213115, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=24.0506329113924, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46294; Mature: 46163

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLPSARQPGRAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFAT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FAVGFFARPLGGIVFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPDYATVGMLAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLRIVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLLAFRAVTSMDK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASAVLLIVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSAWGLV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHHHHHHHE
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFVGQPISAWIAER
EHHCCCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IGGARFLKLAAALAMLSPYPMFMLVQGGTSASLVTGIAIAVVCMSGFYSVIAGFVSGVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGALAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLSGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAAQPAAAEALGTH
HHHCCCCHHHHHCCCC
>Mature Secondary Structure 
TQSLPSARQPGRAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDRFVSTMASFAT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FAVGFFARPLGGIVFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPDYATVGMLAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLRIVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLLAFRAVTSMDK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASAVLLIVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSAWGLV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHHHHHHHE
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFVGQPISAWIAER
EHHCCCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IGGARFLKLAAALAMLSPYPMFMLVQGGTSASLVTGIAIAVVCMSGFYSVIAGFVSGVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGALAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLSGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAAQPAAAEALGTH
HHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]