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The map label for this gene is lipA [H]

Identifier: 161524610

GI number: 161524610

Start: 1581371

End: 1582336

Strand: Direct

Name: lipA [H]

Synonym: Bmul_1437

Alternate gene names: 161524610

Gene position: 1581371-1582336 (Clockwise)

Preceding gene: 161524609

Following gene: 161524619

Centisome position: 45.86

GC content: 72.77

Gene sequence:

>966_bases
ATGGCTGCCGCGCTCGAACGACCCAGCCTCACCGCGCTCGGCCAGCCGGGCGCGCGCAGCCGCGACAAGCTCGCGCGCAT
TCCCGTGCGCGTCGAGCCCGCCGACGGTGCGGCGCTGCCCAAGCCGCCGTGGCTGCGCGCACGGCCGATGATGAGCGCGG
CCGTCGCCGACATGGCGGCCGTGCTGCGCGCGCATCGGCTGCATTCCGTATGCGAGGAAGCGATGTGTCCGAACATCGGC
GAGTGTTTTGCGCAACGCACGGCGACCTTCATGATCATGGGCGGGCTCTGCACGCGCCGCTGCCCGTTCTGCGACGTCGC
GCACGGCCGTCCGGAGCCGCTCGATCCGGACGAGCCCGCCCGGCTCGCGGAGGCCGTCGCCGCGCTCGCGCTGCGCTATG
TCGTGATCACGTCGGTCGACCGCGACGATCTGCGCGACGGCGGCGCGGCGCATTTCGCCGCCTGCATCGCGGCCGTGCGC
GCGAGCGTGCCGGGGATCGGCGTCGAGGTGCTGGTACCCGATTTCCGCGGCCGCATCGATCGTGCGCTCGACGCGCTGTC
GCAGGCATGGCCCGACGTGTTCAACCACAACATCGAGACGGTGCCGTCGCTGTATCGCGCGGCGCGGCCCGGCGCCGACT
ATCGCGGCTCGCTCGACCTGCTCGCGCGCGCGAAGGGCGCGCGGCCGGCGCTCGTCACGAAATCGGGATTGATGCTCGGG
CTCGGCGAAGGCAACGACGAGGTGCGCGCGACGATGCGCGACCTGCGCGCGCACGGTGTCGATGTCCTGACGCTCGGCCA
GTATCTCGCGCCGTCCGCGCATCATCTGCCGGTGCGGCGCTACGTGAGCCCGGAGGCGTTCGCGGCATGGCGCGACGAAG
GGCTCGCGCTCGGTTTCGCGGAGGTGGTCGCCGGCCCGCTCGTGCGCTCGTCGTATCACGCGGCCGACGTGCTGGTGCCC
GCGTAG

Upstream 100 bases:

>100_bases
GCGTGCGCACCGAGCTGATCGCCGGCAGCGGGCACATGGTGCAGATGGAAGCGGCGGCCGACGTGAACCGGCTGATCGTC
GCGTTTCTCGGAGACTGACG

Downstream 100 bases:

>100_bases
CGGGCCGCGGCTCAGGCGCCGGCGGACACGTGCAGGCGGCTCACGCGGCGCAGGTACAGCGCGAGCCCGACGCCCGCGAG
CATCAGGCTGACCGTGTTCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAAALERPSLTALGQPGARSRDKLARIPVRVEPADGAALPKPPWLRARPMMSAAVADMAAVLRAHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLAEAVAALALRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLVPDFRGRIDRALDALSQAWPDVFNHNIETVPSLYRAARPGADYRGSLDLLARAKGARPALVTKSGLMLG
LGEGNDEVRATMRDLRAHGVDVLTLGQYLAPSAHHLPVRRYVSPEAFAAWRDEGLALGFAEVVAGPLVRSSYHAADVLVP
A

Sequences:

>Translated_321_residues
MAAALERPSLTALGQPGARSRDKLARIPVRVEPADGAALPKPPWLRARPMMSAAVADMAAVLRAHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLAEAVAALALRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLVPDFRGRIDRALDALSQAWPDVFNHNIETVPSLYRAARPGADYRGSLDLLARAKGARPALVTKSGLMLG
LGEGNDEVRATMRDLRAHGVDVLTLGQYLAPSAHHLPVRRYVSPEAFAAWRDEGLALGFAEVVAGPLVRSSYHAADVLVP
A
>Mature_320_residues
AAALERPSLTALGQPGARSRDKLARIPVRVEPADGAALPKPPWLRARPMMSAAVADMAAVLRAHRLHSVCEEAMCPNIGE
CFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLAEAVAALALRYVVITSVDRDDLRDGGAAHFAACIAAVRA
SVPGIGVEVLVPDFRGRIDRALDALSQAWPDVFNHNIETVPSLYRAARPGADYRGSLDLLARAKGARPALVTKSGLMLGL
GEGNDEVRATMRDLRAHGVDVLTLGQYLAPSAHHLPVRRYVSPEAFAAWRDEGLALGFAEVVAGPLVRSSYHAADVLVPA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=291, Percent_Identity=46.7353951890034, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=47.2, Blast_Score=224, Evalue=7e-59,
Organism=Escherichia coli, GI1786846, Length=298, Percent_Identity=61.4093959731544, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=44.9826989619377, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=43.5087719298246, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI221513272, Length=300, Percent_Identity=45.6666666666667, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34295; Mature: 34164

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAALERPSLTALGQPGARSRDKLARIPVRVEPADGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLRAHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCHH
RLAEAVAALALRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLVPDFRGRID
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHH
RALDALSQAWPDVFNHNIETVPSLYRAARPGADYRGSLDLLARAKGARPALVTKSGLMLG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCEEE
LGEGNDEVRATMRDLRAHGVDVLTLGQYLAPSAHHLPVRRYVSPEAFAAWRDEGLALGFA
ECCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCCEEEHH
EVVAGPLVRSSYHAADVLVPA
HHHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure 
AAALERPSLTALGQPGARSRDKLARIPVRVEPADGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLRAHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCHH
RLAEAVAALALRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLVPDFRGRID
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHH
RALDALSQAWPDVFNHNIETVPSLYRAARPGADYRGSLDLLARAKGARPALVTKSGLMLG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEECCCCEEE
LGEGNDEVRATMRDLRAHGVDVLTLGQYLAPSAHHLPVRRYVSPEAFAAWRDEGLALGFA
ECCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCCEEEHH
EVVAGPLVRSSYHAADVLVPA
HHHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA