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The map label for this gene is acoB [H]

Identifier: 161524608

GI number: 161524608

Start: 1579244

End: 1580248

Strand: Direct

Name: acoB [H]

Synonym: Bmul_1435

Alternate gene names: 161524608

Gene position: 1579244-1580248 (Clockwise)

Preceding gene: 161524607

Following gene: 161524609

Centisome position: 45.8

GC content: 67.76

Gene sequence:

>1005_bases
ATGGCAAGGAAGATCACTTATTCGCAGGCGATCAACGAGGCGCTCGCGCAGGAGATGGCGCGCGACGACAGCGTGATCGT
GATGGGCGAGGACAACGCGGGCGGCGCGGGCGCGCCGGGCGAGGACGACGCATGGGGCGGCGTGCTCGGCGTGACGAAGG
GGCTGTTCCACAAGTTTCCGGGCCGCGTGCTCGACACGCCGCTGTCCGAAGGCGGCTACATCGGCGCGGCGGTCGGCGCC
GCCGCGTGCGGGATGCGGCCCGTCGCCGAGCTGATGTTCGTCGACTTCATGGGCGTGTGCTTCGACCAGATCTTCAACCA
GGCCGCGAAATTCCGCTACATGTTCGGCGGCAAGGCCGTCACGCCGGTCGTGATCCGCGCGATGTACGGCGCGGGCCTGC
GCGCGGCCGCGCAGCACTCGCAGATGCTCACGTCGCTGTTTACGCACATTCCGGGGCTGAAGGTCGTGTGCCCGTCGACG
CCGTACGACGCGAAGGGGCTGCTGATCCAGTCGATCCGCGACAACGATCCGGTAATCTTCCTCGAGCACAAGCTGCTCTA
CACGCGCGAGGGCGACGTGCCGGAGGAATCCTATGCGATTCCGTTCGGCGAGGCGAACATCGTGCGCGACGGCGACGACG
CGACGATCGTCACCTACGGCCGGATGGTGCATGTCGCGATGGACGCCGCCGCGAAGCTCGCGAAGGACGGCATTCAGGTC
GACGTGATCGACCTGCGCACGACGTCGCCGCTCGACGAGGAAACGATTCTCGAAAGCGCGGCGCGCACCGGGCGCGTGGT
GGTCGTCGACGAAGCGAACCCGCGCTGCTCGATCGCGACCGACATCGCCGCGCTCGTCGCGCAGCGCGCGTTCCGTTCGC
TGCAGGCGCCGATCGAGCTCGTGACCGCGCCGCATACGCCCGCGCCGTTCGCCGGCGTGCTCGAGGATTTGTATATCCCG
TCTGCCGATGCGATCGCGCAGGCCGTGCTGAAAACGAGGAGCTGA

Upstream 100 bases:

>100_bases
GACCTGCTGACCGACGTCTACGTGTCGTATCCGTGAGCGCGGCCGAACCGCGCGCAATGCAACGGCCGACACGGCGAACC
GAACATTCCAGGAGACAGAC

Downstream 100 bases:

>100_bases
GACGATGTCGATTCACATGATCACGATGCCCAAGTGGGGGCTGTCGATGGAGCAGGGGCAGGTCAACGGCTGGCTGAAGT
CGGTCGGCGAACGCGTGTCG

Product: transketolase central region

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGA
AACGMRPVAELMFVDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPST
PYDAKGLLIQSIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANIVRDGDDATIVTYGRMVHVAMDAAAKLAKDGIQV
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLQAPIELVTAPHTPAPFAGVLEDLYIP
SADAIAQAVLKTRS

Sequences:

>Translated_334_residues
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGA
AACGMRPVAELMFVDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPST
PYDAKGLLIQSIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANIVRDGDDATIVTYGRMVHVAMDAAAKLAKDGIQV
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLQAPIELVTAPHTPAPFAGVLEDLYIP
SADAIAQAVLKTRS
>Mature_333_residues
ARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGAA
ACGMRPVAELMFVDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTP
YDAKGLLIQSIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANIVRDGDDATIVTYGRMVHVAMDAAAKLAKDGIQVD
VIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLQAPIELVTAPHTPAPFAGVLEDLYIPS
ADAIAQAVLKTRS

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=337, Percent_Identity=39.7626112759644, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI291084858, Length=337, Percent_Identity=37.9821958456973, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4557353, Length=337, Percent_Identity=37.6854599406528, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI34101272, Length=337, Percent_Identity=37.6854599406528, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI225637463, Length=295, Percent_Identity=28.4745762711864, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI225637461, Length=295, Percent_Identity=28.4745762711864, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637459, Length=295, Percent_Identity=28.4745762711864, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=337, Percent_Identity=40.6528189910979, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17506935, Length=334, Percent_Identity=40.7185628742515, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=339, Percent_Identity=38.6430678466077, Blast_Score=230, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21358145, Length=336, Percent_Identity=38.6904761904762, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24650940, Length=336, Percent_Identity=38.6904761904762, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI160714832, Length=332, Percent_Identity=35.8433734939759, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI160714828, Length=332, Percent_Identity=35.8433734939759, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24650943, Length=103, Percent_Identity=39.8058252427184, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650945, Length=103, Percent_Identity=39.8058252427184, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35570; Mature: 35439

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFP
CCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GRVLDTPLSEGGYIGAAVGAAACGMRPVAELMFVDFMGVCFDQIFNQAAKFRYMFGGKAV
CHHHCCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCH
TPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTPYDAKGLLIQSIRDNDPVIF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCEEE
LEHKLLYTREGDVPEESYAIPFGEANIVRDGDDATIVTYGRMVHVAMDAAAKLAKDGIQV
EEEEEEEEECCCCCCCCEECCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCEE
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLQAPIEL
EEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VTAPHTPAPFAGVLEDLYIPSADAIAQAVLKTRS
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFP
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GRVLDTPLSEGGYIGAAVGAAACGMRPVAELMFVDFMGVCFDQIFNQAAKFRYMFGGKAV
CHHHCCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCH
TPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTPYDAKGLLIQSIRDNDPVIF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCEEE
LEHKLLYTREGDVPEESYAIPFGEANIVRDGDDATIVTYGRMVHVAMDAAAKLAKDGIQV
EEEEEEEEECCCCCCCCEECCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCEE
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLQAPIEL
EEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VTAPHTPAPFAGVLEDLYIPSADAIAQAVLKTRS
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]