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The map label for this gene is dmlR [H]

Identifier: 161524570

GI number: 161524570

Start: 1540465

End: 1541376

Strand: Direct

Name: dmlR [H]

Synonym: Bmul_1397

Alternate gene names: 161524570

Gene position: 1540465-1541376 (Clockwise)

Preceding gene: 161524565

Following gene: 161524571

Centisome position: 44.67

GC content: 68.53

Gene sequence:

>912_bases
ATGGATCGCTTCAAGCAGATCGAGACGTTCGTGCGCGTCGCGGACGCGGGCAGCCTCGCGGCGGCGGCACTCGAGGAGGG
CGTGTCGCCGGTCGTGCTCGGCCGGCGCATCGACGCACTCGAGAAGCGGCTCGGCGTCAAGCTGATGTACCGGTCGACGC
GGCGGCTCGTCGTCAGCGAGGAGGGCGCCGCGTTCCTCGAACGCTGTCGCGGGCTGCTGTCCGAATGGGATCAGGCCGAG
AACGAGCTGGCCGCGGGACGGCGCGCGGTCAGCGGTCACCTGATCGTATCGGCGCCGGCCGCGTTCGGCCGCAAGCACGT
CGCGCCGCACGCGCCGGGCTTTCTCGCCGACAAGCCCGAGATGCAGCTGTCGTTCAACCTGACCGATCGCGTGGTCGATC
TCGTGCGCGAAGGCTACGACCTGTCGATCCGGATCGGCGGCGCGGTGGATCCGAACTTCGTCGCGGTGAAGCTCGCATCG
AACCGGCGCGTCGTGTGCGGCACGCCGGACTATTTCCGCCGGCACGGCCGCCCGAAGTCGCTCGACGATCTGCTCAAGCA
CAACTGCCTCGCGTTCAACCTGCAGGGCGGGCAGAACCGCGGCTGGTACTTTCAGCGCCACGGCAAGATCGTGACGATGC
GCGTGACCGGCAATCTCGACTGCAACGACGGCGAGCTGCTGCACCGCTGGGTCGCCGAGGGGCTCGGGCTCGGCTGGCGC
TCGACATGGGAAATCGCCGCGCAGCTCGAGACGGGCGAACTCGAAACGGTGCTCGACGAGTATGCGCTGCCCGACTACGA
CATCCTCGCCGTCTATCCGCAGCAGCGCTACGTGCCGGCGCGCGTGCGCTACTTCATCGACTACTTGCGCGACGCGTACG
CGCAGCCGGGGTACTGGAGCACGTCGCCGTAA

Upstream 100 bases:

>100_bases
AAAGGTTTGATAAGATCAGCCGAAGTCGCTTATTTCGAAACTAAAAGTATGGAATGACGGCCGGACGGGCGTTCCGCCGG
CGTCGGAACGGAGACGGGAG

Downstream 100 bases:

>100_bases
TGGGCGGGCGCGGTGCTTCAGCGAGCGGCCGTGCGCGCCAGTTGCGGTTTTTTTCGCTTCGGGCCCGTGCCGCGGGAATA
AACTCGACGCAATGGCGGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPDYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIVTMRVTGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDAYAQPGYWSTSP

Sequences:

>Translated_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPDYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIVTMRVTGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDAYAQPGYWSTSP
>Mature_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEEGAAFLERCRGLLSEWDQAE
NELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPEMQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPDYFRRHGRPKSLDDLLKHNCLAFNLQGGQNRGWYFQRHGKIVTMRVTGNLDCNDGELLHRWVAEGLGLGWR
STWEIAAQLETGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDAYAQPGYWSTSP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=36.3013698630137, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=30.8771929824561, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=30.5555555555556, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=29.6819787985866, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.0102389078498, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=25.9259259259259, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.972027972028, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=20.6081081081081, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34020; Mature: 34020

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCC
MQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPDYFRRHGRPKS
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCHHHHHCCCCCC
LDDLLKHNCLAFNLQGGQNRGWYFQRHGKIVTMRVTGNLDCNDGELLHRWVAEGLGLGWR
HHHHHHCCEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
STWEIAAQLETGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDAYAQPGYWS
CCCCEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TSP
CCC
>Mature Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
EGAAFLERCRGLLSEWDQAENELAAGRRAVSGHLIVSAPAAFGRKHVAPHAPGFLADKPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCC
MQLSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPDYFRRHGRPKS
EEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCHHHHHCCCCCC
LDDLLKHNCLAFNLQGGQNRGWYFQRHGKIVTMRVTGNLDCNDGELLHRWVAEGLGLGWR
HHHHHHCCEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
STWEIAAQLETGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDAYAQPGYWS
CCCCEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TSP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]