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The map label for this gene is ansA [H]

Identifier: 161524565

GI number: 161524565

Start: 1534669

End: 1535691

Strand: Direct

Name: ansA [H]

Synonym: Bmul_1392

Alternate gene names: 161524565

Gene position: 1534669-1535691 (Clockwise)

Preceding gene: 161524561

Following gene: 161524570

Centisome position: 44.5

GC content: 70.97

Gene sequence:

>1023_bases
ATGAATACGCTGACTCCCGCTTCTTCTTCGTCCGCGGCGCACCTGCCGCGCATTGCCGTTCTCGCAACCGGCGGCACGAT
CGCCGGCGCCGCGCCCGACGCGACGAGCACGGCCGGCTATCAGGCCGGCGCGCTCGGCGTCAATTTCCTGCTCGACGCGG
TACCGGCGCTCGCGTCGGTCGCGCGCATTCACGCCGAGCAGATCGCCAGCATCGACAGCAAGGATCTCGCGCTGTCGCTC
TGGAATACGCTCGCCGCGCGGATCGACGCGCTGATGGCCGATCCGGCCGTCGACGGCATCGTCATCACGCACGGCACCGA
CACGCTCGAGGAAACCGCCTATGCGCTGCATCTGGTCGTGGCCGGCGACAAGCCGGTCGTGCTGACCGCCGCGATGCGGC
CGGCCACCGCGCTGTCGTCGGACGGGCCGCTGAACCTGCTGAACGCCGTGACGCTCGCCGGGCATCCGGCCGCGCGCGGG
CAGGGCGTGCTCGTCGCGTTCAACAACCGGATCCATGCGGCGCGCGACGTCGTGAAGACGAGCACCTACGCGGTCGACGC
GTTCCAGTCGCCGGAGCTTGGCGCGCTCGGCTGGGTGCAGGACGGCCGCGTCGAATTCGCGCGCCGCGTCACGCGTGCGC
GCGATGCACAACTGACGATTCCCGAGACGTGGCCGCCGGTCGACGTGGTGACGAGCTATGCGGGCGTCACGCGCACGGCC
GTCGATGCGCTCGTCGCGGCCGGCGTGCACGGGCTCGTCGTCGCGGGCACCGGCAACGGCTCGATCCATGCGACGCTGCA
GACGGCGCTGGCCGACGCGGTGAACGCCGGGGTCGCGGTGGTGCGCGCGTCGCGCGTCGGTTCCGGTCACGTGATGCGCA
ACGGCGCCGCGAACGACGATGCGTTCGGCTTCGTCAGCGCCGGCTCGTTGAGCCCGTTCAAGGCGCGCGTGCTGCTGATG
CTCGCACTCGCGAACGGCATTCGCGAGCGCGACGCGTTGCAGCAGCTGTTCGATCGCTGCTGA

Upstream 100 bases:

>100_bases
CGCGCCGGCGATCGGGATAAACCATGAGCGGCCCGCGTCGCATGCGTTTGTTCGCACCGAATCGCTCGAATCCGGGCCGC
GAGCCATTACAATCGCGGCC

Downstream 100 bases:

>100_bases
TCCACACGCGGCCGCGTGCCGGCCGAATCGATGCCGTAAACGACAAAGGCAGGCCGAACGGCCTGCCTTTTCTCATGTCG
ACGCGCGCAAACGCTCAGGC

Product: type II L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNTLTPASSSSAAHLPRIAVLATGGTIAGAAPDATSTAGYQAGALGVNFLLDAVPALASVARIHAEQIASIDSKDLALSL
WNTLAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVAGDKPVVLTAAMRPATALSSDGPLNLLNAVTLAGHPAARG
QGVLVAFNNRIHAARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRARDAQLTIPETWPPVDVVTSYAGVTRTA
VDALVAAGVHGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGAANDDAFGFVSAGSLSPFKARVLLM
LALANGIRERDALQQLFDRC

Sequences:

>Translated_340_residues
MNTLTPASSSSAAHLPRIAVLATGGTIAGAAPDATSTAGYQAGALGVNFLLDAVPALASVARIHAEQIASIDSKDLALSL
WNTLAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVAGDKPVVLTAAMRPATALSSDGPLNLLNAVTLAGHPAARG
QGVLVAFNNRIHAARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRARDAQLTIPETWPPVDVVTSYAGVTRTA
VDALVAAGVHGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGAANDDAFGFVSAGSLSPFKARVLLM
LALANGIRERDALQQLFDRC
>Mature_340_residues
MNTLTPASSSSAAHLPRIAVLATGGTIAGAAPDATSTAGYQAGALGVNFLLDAVPALASVARIHAEQIASIDSKDLALSL
WNTLAARIDALMADPAVDGIVITHGTDTLEETAYALHLVVAGDKPVVLTAAMRPATALSSDGPLNLLNAVTLAGHPAARG
QGVLVAFNNRIHAARDVVKTSTYAVDAFQSPELGALGWVQDGRVEFARRVTRARDAQLTIPETWPPVDVVTSYAGVTRTA
VDALVAAGVHGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGAANDDAFGFVSAGSLSPFKARVLLM
LALANGIRERDALQQLFDRC

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=227, Percent_Identity=29.0748898678414, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789327, Length=335, Percent_Identity=42.6865671641791, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1788065, Length=305, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558278, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=232, Percent_Identity=30.1724137931034, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=336, Percent_Identity=35.7142857142857, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323187, Length=336, Percent_Identity=35.7142857142857, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323186, Length=336, Percent_Identity=35.7142857142857, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323184, Length=336, Percent_Identity=35.7142857142857, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=33.5329341317365, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24645530, Length=227, Percent_Identity=28.6343612334802, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639609, Length=252, Percent_Identity=25.7936507936508, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34784; Mature: 34784

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLTPASSSSAAHLPRIAVLATGGTIAGAAPDATSTAGYQAGALGVNFLLDAVPALASV
CCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARIHAEQIASIDSKDLALSLWNTLAARIDALMADPAVDGIVITHGTDTLEETAYALHLVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHEEEEEEEE
AGDKPVVLTAAMRPATALSSDGPLNLLNAVTLAGHPAARGQGVLVAFNNRIHAARDVVKT
ECCCCEEEEECCCCCHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHH
STYAVDAFQSPELGALGWVQDGRVEFARRVTRARDAQLTIPETWPPVDVVTSYAGVTRTA
HHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHH
VDALVAAGVHGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGAANDD
HHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCC
AFGFVSAGSLSPFKARVLLMLALANGIRERDALQQLFDRC
CCEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLTPASSSSAAHLPRIAVLATGGTIAGAAPDATSTAGYQAGALGVNFLLDAVPALASV
CCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARIHAEQIASIDSKDLALSLWNTLAARIDALMADPAVDGIVITHGTDTLEETAYALHLVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHEEEEEEEE
AGDKPVVLTAAMRPATALSSDGPLNLLNAVTLAGHPAARGQGVLVAFNNRIHAARDVVKT
ECCCCEEEEECCCCCHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHH
STYAVDAFQSPELGALGWVQDGRVEFARRVTRARDAQLTIPETWPPVDVVTSYAGVTRTA
HHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHH
VDALVAAGVHGLVVAGTGNGSIHATLQTALADAVNAGVAVVRASRVGSGHVMRNGAANDD
HHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCC
AFGFVSAGSLSPFKARVLLMLALANGIRERDALQQLFDRC
CCEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]