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The map label for this gene is thiC [H]

Identifier: 161485683

GI number: 161485683

Start: 251926

End: 253212

Strand: Reverse

Name: thiC [H]

Synonym: MM_0182

Alternate gene names: 161485683

Gene position: 253212-251926 (Counterclockwise)

Preceding gene: 21226286

Following gene: 21226282

Centisome position: 6.18

GC content: 51.2

Gene sequence:

>1287_bases
ATGACGCTGATGGAAGATGCAAAAAAGGGGGTCATCACCCCTGAAATCGAAGCCGTGGCAAAAGCTGAAGGAATAGACGC
CGAGATTGTCCGTTCCTGCGTTGCAAAAGGGCTGGTCGCCATTCCAAAGAATGCCAGGCGAGACACTCTCCCTGTCGGTA
TTGGAAAGTACATGAGCACAAAAATAAATGCCAATGTAGGGACCTCAAGGGACTGCATCGATATAGATGCTGAAATTGAA
AAAGCAAAAGCTGCAGAAGCCTTCGGAGCCCATGCAGTGATGGACCTCTCAACAGGAGGAGACCTGGACGAGATCCGCAC
CCGCATCCTTAAAGCCGTAAACATCCCTGTAGGGACTGTCCCTATCTATCAGGCTGCAGCTTCCCGGAAAATTGTGGTGG
AAATGAGCTCTGACGACATGTTCAATGCCGTCAGGAAACATGCCGAACAGGGAGTGGACTTTGTAACCGTACATGCCGGG
GTCAACCTGAACTCCCTGGAGCGCCTTCGCCAGAGTGACCGGATTATGAATGTTGTCAGCCGCGGAGGCTCTTTTACCCT
TGCCTGGATGCTGCACAACGGGGAAGACAACCCCTTTTATGCCGAATTTGACTATCTTCTTGAAATCGCAAAAGAATATG
ATATGACCCTGAGCCTTGGAGACGGCATGCGCCCCGGATGTATAGCAGACGCTTCCGACCGTCCCAAGTTCATGGAATTT
ATCACCCTCGGAGAGCTTGTAAAGCGCTCAAGGGAGGCAAATGTCCAGACTTTTGTTGAAGGACCCGGGCATGTCCCCTT
AAACGAAATTGAACTCAGCGTCCGTGGCATGAAAGAACTCTGTGACGGAGCCCCCCTTTACCTTCTAGGCCCGCTTGTCA
CTGATATTGCTCCCGGTTTTGACCACATCACAGGCGCAATAGGAGGAGCAGTAGCAGGTATGCACGGAACTGACTTCCTC
TGTATGGTAACTCCCTCAGAACACCTTGCTCTTCCATCTATCGAAGATATTAAAGAGGGACTGCTTGTAACAAAGCTTGC
AGCTCACACCATAGACCTCATAAAAGAAGGGCCAAGAGAACGTGCATGGAAGCAGGATACAGCCATGGCCTATGCCCGAA
GAGACCTTGACTGGGAAAAGCAGTTCGAGCTAGCAATCGACGGCGACCGCGCCCGTAAAATTCGGGACGCCAGAAAGACG
GAAAGTGATGCCTGCTCCATGTGCGGGGAGCTCTGCGCAGTTAAAATCGTAAAGGAAGCTTTCGGGGAAAAGAAGGAAGA
GGAGTAA

Upstream 100 bases:

>100_bases
CATTCAGATTCAGGACTTAAACGTTTATGGCTGGAATCTTCAGGACCTGAGTACTTCAGGACTGGAATACTACTCATCCA
CCTCACATACGGAGCAAAAA

Downstream 100 bases:

>100_bases
AGACAGAAAAGAGTAAAGACCGAAAAAAGTAAAGGCAGAAGAAGCAATTTCTTTTATTTCCTTTTTCTTTCAATTATCTA
ATTTTTTCTTCTTTCCAGTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTLMEDAKKGVITPEIEAVAKAEGIDAEIVRSCVAKGLVAIPKNARRDTLPVGIGKYMSTKINANVGTSRDCIDIDAEIE
KAKAAEAFGAHAVMDLSTGGDLDEIRTRILKAVNIPVGTVPIYQAAASRKIVVEMSSDDMFNAVRKHAEQGVDFVTVHAG
VNLNSLERLRQSDRIMNVVSRGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPKFMEF
ITLGELVKRSREANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDIAPGFDHITGAIGGAVAGMHGTDFL
CMVTPSEHLALPSIEDIKEGLLVTKLAAHTIDLIKEGPRERAWKQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKT
ESDACSMCGELCAVKIVKEAFGEKKEEE

Sequences:

>Translated_428_residues
MTLMEDAKKGVITPEIEAVAKAEGIDAEIVRSCVAKGLVAIPKNARRDTLPVGIGKYMSTKINANVGTSRDCIDIDAEIE
KAKAAEAFGAHAVMDLSTGGDLDEIRTRILKAVNIPVGTVPIYQAAASRKIVVEMSSDDMFNAVRKHAEQGVDFVTVHAG
VNLNSLERLRQSDRIMNVVSRGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPKFMEF
ITLGELVKRSREANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDIAPGFDHITGAIGGAVAGMHGTDFL
CMVTPSEHLALPSIEDIKEGLLVTKLAAHTIDLIKEGPRERAWKQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKT
ESDACSMCGELCAVKIVKEAFGEKKEEE
>Mature_427_residues
TLMEDAKKGVITPEIEAVAKAEGIDAEIVRSCVAKGLVAIPKNARRDTLPVGIGKYMSTKINANVGTSRDCIDIDAEIEK
AKAAEAFGAHAVMDLSTGGDLDEIRTRILKAVNIPVGTVPIYQAAASRKIVVEMSSDDMFNAVRKHAEQGVDFVTVHAGV
NLNSLERLRQSDRIMNVVSRGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPKFMEFI
TLGELVKRSREANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDIAPGFDHITGAIGGAVAGMHGTDFLC
MVTPSEHLALPSIEDIKEGLLVTKLAAHTIDLIKEGPRERAWKQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKTE
SDACSMCGELCAVKIVKEAFGEKKEEE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=454, Percent_Identity=41.6299559471366, Blast_Score=327, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46739; Mature: 46608

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMEDAKKGVITPEIEAVAKAEGIDAEIVRSCVAKGLVAIPKNARRDTLPVGIGKYMST
CCCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHE
KINANVGTSRDCIDIDAEIEKAKAAEAFGAHAVMDLSTGGDLDEIRTRILKAVNIPVGTV
EECCCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
PIYQAAASRKIVVEMSSDDMFNAVRKHAEQGVDFVTVHAGVNLNSLERLRQSDRIMNVVS
HHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
RGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPKFMEF
CCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHH
ITLGELVKRSREANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDIAPGF
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
DHITGAIGGAVAGMHGTDFLCMVTPSEHLALPSIEDIKEGLLVTKLAAHTIDLIKEGPRE
HHHHHHHHHHHCCCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAWKQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKTESDACSMCGELCAVKIVKEA
HHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FGEKKEEE
HCCCCCCC
>Mature Secondary Structure 
TLMEDAKKGVITPEIEAVAKAEGIDAEIVRSCVAKGLVAIPKNARRDTLPVGIGKYMST
CCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHE
KINANVGTSRDCIDIDAEIEKAKAAEAFGAHAVMDLSTGGDLDEIRTRILKAVNIPVGTV
EECCCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
PIYQAAASRKIVVEMSSDDMFNAVRKHAEQGVDFVTVHAGVNLNSLERLRQSDRIMNVVS
HHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
RGGSFTLAWMLHNGEDNPFYAEFDYLLEIAKEYDMTLSLGDGMRPGCIADASDRPKFMEF
CCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHH
ITLGELVKRSREANVQTFVEGPGHVPLNEIELSVRGMKELCDGAPLYLLGPLVTDIAPGF
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
DHITGAIGGAVAGMHGTDFLCMVTPSEHLALPSIEDIKEGLLVTKLAAHTIDLIKEGPRE
HHHHHHHHHHHCCCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAWKQDTAMAYARRDLDWEKQFELAIDGDRARKIRDARKTESDACSMCGELCAVKIVKEA
HHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FGEKKEEE
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]