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The map label for this gene is ispF

Identifier: 160902632

GI number: 160902632

Start: 1257432

End: 1257920

Strand: Direct

Name: ispF

Synonym: Pmob_1172

Alternate gene names: 160902632

Gene position: 1257432-1257920 (Clockwise)

Preceding gene: 160902631

Following gene: 160902633

Centisome position: 57.96

GC content: 31.29

Gene sequence:

>489_bases
ATGTTAAAAATTGGGTTTGGTTACGATGTTCATCCTTTTGCTGAAAATAGAAGATTAATATTGGGTGGAGTTGTAATTAA
TCATCCAAAAGGTTATGGATTATTGGGCCATTCGGATGGAGACGTTTTTTTTCATGCATTAATTGATAGTTTATTAGGGA
TGTGCGGTTTGGGGTCAATAGGGGAGTACTTTCCTGAAAGTAAAGAGTTTGAAAATATTAGTAGCTCAATCCTATTAGAA
AAGACTATAGATTTGATTAATAAAAGATATATTGTTAAGATAAATAATATAGATGTGGTGATCATATCTAAGAGTGTAAA
TATCAGTTCAATAACAGGTCAAATCAAAAATAATACCTCGTGTATCTTAAATTTAGAAGTAAGTCGAATAAACTTAAAAG
GGAAAAGTGGTAATGGTCTGGGGGTTGGAGGAAATGACCAAGGTATAGAAGTTTATTGTACCATTTTAGGTGAGATAGAT
GAAATATAA

Upstream 100 bases:

>100_bases
TGAATTTGATATATCCGATGAGGAAAAACCTACCATTTTAAAACCTTTGGAAGACAACAGTTACATGTTCATAATAATGC
CAAAAAGGAAAAGTTGAAAC

Downstream 100 bases:

>100_bases
ACCTGTTCATCGGTTCGATATGTCTCCTAAAGATATGATAAAAGTGCAAAAACAATTAGCTAAAGAGGTAAAATTAATCC
ATTTTTCGGATGAACCAAAC

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLKIGFGYDVHPFAENRRLILGGVVINHPKGYGLLGHSDGDVFFHALIDSLLGMCGLGSIGEYFPESKEFENISSSILLE
KTIDLINKRYIVKINNIDVVIISKSVNISSITGQIKNNTSCILNLEVSRINLKGKSGNGLGVGGNDQGIEVYCTILGEID
EI

Sequences:

>Translated_162_residues
MLKIGFGYDVHPFAENRRLILGGVVINHPKGYGLLGHSDGDVFFHALIDSLLGMCGLGSIGEYFPESKEFENISSSILLE
KTIDLINKRYIVKINNIDVVIISKSVNISSITGQIKNNTSCILNLEVSRINLKGKSGNGLGVGGNDQGIEVYCTILGEID
EI
>Mature_162_residues
MLKIGFGYDVHPFAENRRLILGGVVINHPKGYGLLGHSDGDVFFHALIDSLLGMCGLGSIGEYFPESKEFENISSSILLE
KTIDLINKRYIVKINNIDVVIISKSVNISSITGQIKNNTSCILNLEVSRINLKGKSGNGLGVGGNDQGIEVYCTILGEID
EI

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=35.2564102564103, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PETMO (A9BHL8)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568213.1
- ProteinModelPortal:   A9BHL8
- SMR:   A9BHL8
- GeneID:   5756578
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1172
- HOGENOM:   HBG630070
- OMA:   ITIICEY
- BioCyc:   PMOB403833:PMOB_1172-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17568; Mature: 17568

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGFGYDVHPFAENRRLILGGVVINHPKGYGLLGHSDGDVFFHALIDSLLGMCGLGSI
CEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCH
GEYFPESKEFENISSSILLEKTIDLINKRYIVKINNIDVVIISKSVNISSITGQIKNNTS
HHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCEEEEEEEECCCCE
CILNLEVSRINLKGKSGNGLGVGGNDQGIEVYCTILGEIDEI
EEEEEEEEEEEEECCCCCCCEECCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MLKIGFGYDVHPFAENRRLILGGVVINHPKGYGLLGHSDGDVFFHALIDSLLGMCGLGSI
CEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCH
GEYFPESKEFENISSSILLEKTIDLINKRYIVKINNIDVVIISKSVNISSITGQIKNNTS
HHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCEEEEEEEECCCCE
CILNLEVSRINLKGKSGNGLGVGGNDQGIEVYCTILGEIDEI
EEEEEEEEEEEEECCCCCCCEECCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA