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The map label for this gene is nfi

Identifier: 160902633

GI number: 160902633

Start: 1257910

End: 1258575

Strand: Direct

Name: nfi

Synonym: Pmob_1173

Alternate gene names: 160902633

Gene position: 1257910-1258575 (Clockwise)

Preceding gene: 160902632

Following gene: 160902634

Centisome position: 57.98

GC content: 34.68

Gene sequence:

>666_bases
ATGAAATATAAACCTGTTCATCGGTTCGATATGTCTCCTAAAGATATGATAAAAGTGCAAAAACAATTAGCTAAAGAGGT
AAAATTAATCCATTTTTCGGATGAACCAAACTTAGTAAGCGGTGTTGATCTTTCCTTTCCAAAAGACGAAGGATTAGCTG
TAATTGTAACAATGGATTTTAAAAAATTATCGGTGATCGATGTAACATATGCCGTGGATAAGATTACCTTACCATATATA
CCTGGCCTTCTTGCTTTTAGAGAGCTTCCTATCTTTTTAAAGGCTTGGGAAAAACTTGAAATTGAACCTGATATAGTTTT
TTTTGATGGTCAAGGCTATGCCCATCCTAGAAGGATGGGCATAGCTACACATGCGTCTTTTTTTATTGAAAAGCCTACTA
TAGGAATTGCTAAATCTAAGTTAATTGGTGAATACGAAGAACCCGGAAAGAAAAAAGGTGAATTCACATTTCTGTACCAT
AAAGATGAGAAAATAGGAATAGTGTTGAGAACAAGAGACAATGTGAAACCTGTATTTGTATCTCCTGGCAACTTGGTTGA
TTTCAATAACGCTTTAGATTTCACTTATCATTTCGCCACAAAGTATAAAATTCCAGAGATAACTCGAAAGGCACACTTAT
ACACTCAAAGTTTAAAGCAGAGGTGA

Upstream 100 bases:

>100_bases
GTAAGTCGAATAAACTTAAAAGGGAAAAGTGGTAATGGTCTGGGGGTTGGAGGAAATGACCAAGGTATAGAAGTTTATTG
TACCATTTTAGGTGAGATAG

Downstream 100 bases:

>100_bases
CGTTGTTGACCGAAAAGAACACGCGAAATTTTATATACATCTCAATGATTCTATTTTCGATGGTCATGAATACTCTTGCT
CCTCTTATGACTTCCATTCA

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKYKPVHRFDMSPKDMIKVQKQLAKEVKLIHFSDEPNLVSGVDLSFPKDEGLAVIVTMDFKKLSVIDVTYAVDKITLPYI
PGLLAFRELPIFLKAWEKLEIEPDIVFFDGQGYAHPRRMGIATHASFFIEKPTIGIAKSKLIGEYEEPGKKKGEFTFLYH
KDEKIGIVLRTRDNVKPVFVSPGNLVDFNNALDFTYHFATKYKIPEITRKAHLYTQSLKQR

Sequences:

>Translated_221_residues
MKYKPVHRFDMSPKDMIKVQKQLAKEVKLIHFSDEPNLVSGVDLSFPKDEGLAVIVTMDFKKLSVIDVTYAVDKITLPYI
PGLLAFRELPIFLKAWEKLEIEPDIVFFDGQGYAHPRRMGIATHASFFIEKPTIGIAKSKLIGEYEEPGKKKGEFTFLYH
KDEKIGIVLRTRDNVKPVFVSPGNLVDFNNALDFTYHFATKYKIPEITRKAHLYTQSLKQR
>Mature_221_residues
MKYKPVHRFDMSPKDMIKVQKQLAKEVKLIHFSDEPNLVSGVDLSFPKDEGLAVIVTMDFKKLSVIDVTYAVDKITLPYI
PGLLAFRELPIFLKAWEKLEIEPDIVFFDGQGYAHPRRMGIATHASFFIEKPTIGIAKSKLIGEYEEPGKKKGEFTFLYH
KDEKIGIVLRTRDNVKPVFVSPGNLVDFNNALDFTYHFATKYKIPEITRKAHLYTQSLKQR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=198, Percent_Identity=28.2828282828283, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI87082357, Length=180, Percent_Identity=37.2222222222222, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_PETMO (A9BHL9)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568214.1
- ProteinModelPortal:   A9BHL9
- GeneID:   5756579
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1173
- HOGENOM:   HBG716360
- OMA:   CMRGYRL
- BioCyc:   PMOB403833:PMOB_1173-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25422; Mature: 25422

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKPVHRFDMSPKDMIKVQKQLAKEVKLIHFSDEPNLVSGVDLSFPKDEGLAVIVTMDF
CCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCEEEEEEECC
KKLSVIDVTYAVDKITLPYIPGLLAFRELPIFLKAWEKLEIEPDIVFFDGQGYAHPRRMG
CEEEEEEEEEEEEEEECCCCCHHHHHHHCCHHEEHHHHCCCCCCEEEECCCCCCCHHHCC
IATHASFFIEKPTIGIAKSKLIGEYEEPGKKKGEFTFLYHKDEKIGIVLRTRDNVKPVFV
EEECCEEEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCEEEE
SPGNLVDFNNALDFTYHFATKYKIPEITRKAHLYTQSLKQR
CCCCEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYKPVHRFDMSPKDMIKVQKQLAKEVKLIHFSDEPNLVSGVDLSFPKDEGLAVIVTMDF
CCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCEEEEEEECC
KKLSVIDVTYAVDKITLPYIPGLLAFRELPIFLKAWEKLEIEPDIVFFDGQGYAHPRRMG
CEEEEEEEEEEEEEEECCCCCHHHHHHHCCHHEEHHHHCCCCCCEEEECCCCCCCHHHCC
IATHASFFIEKPTIGIAKSKLIGEYEEPGKKKGEFTFLYHKDEKIGIVLRTRDNVKPVFV
EEECCEEEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCEEEE
SPGNLVDFNNALDFTYHFATKYKIPEITRKAHLYTQSLKQR
CCCCEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA