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The map label for this gene is menD [H]

Identifier: 160877390

GI number: 160877390

Start: 5067766

End: 5069487

Strand: Direct

Name: menD [H]

Synonym: Sbal195_4288

Alternate gene names: 160877390

Gene position: 5067766-5069487 (Clockwise)

Preceding gene: 160877389

Following gene: 160877391

Centisome position: 94.77

GC content: 52.26

Gene sequence:

>1722_bases
ATGCGGACCGAAAATACAGCCACTCTTAATCTTATTTGGGGCGCCCTTATTCTTGAGGAGTTAGCACGTCTTGGCGTGCA
ACATGTGTGTATGGCACCGGGCTCGCGCTCGACGCCGTTAACCCTCGCCGCCGCCCAGCAAACTAAACTCCAGCGTCACC
TGCATTTCGACGAACGTGGACTCGGCTTTATGGCCTTAGGTCTGGCAAAAGCCAGCCGCGCACCGGTCGCTATCATTACC
ACCTCGGGCACCGCAGTCGCTAACTTGTATCCTGCGATTGTTGAAGCTTGGCTAACCCACGTGCCTTTGATCGTCTTAAG
CGGCGATCGTCCACCCGAATTACTCGGCTGCGGCGCCAATCAAGCCATAGTCCAACCGGGGATTTTTGCGAATTACGCTA
CCCAAGTGAATTTGCCAACGCCGGATGCGCATATCGCACCGCAGGCGTTGCTCACCACAGTGGATGAAGCGGTGGCGAAT
CAAACTCGTCCAGTGCACATCAACTGCATGTACCGTGAGCCCTTATATCCGAGTGAACTCAGTGGCGTGATTCTCGATAC
CGAATCGCCTTACCTCAAACCACTGCAAACTTGGCTGCAGCTCGCCCGCCCGTATACCCAATACGGCAAGTGCAAACAAT
TGAGCAGCCCCAGTGACGATGCCATCATGCGGTTCGTCCACGGTAAAGGCGTGATCGTTGTCGGCACTCTCACGCCCGAG
CAAGATCCACAGCAGCTTATCGCGTTATCGCAAAAAATTGGCTGGCCTTTGCTTACCGATGCCCAGTCGCAACTGCGCCA
ACATCCCGCGGCAATAGGCAACATCGACCAACTGCTGCAACATCCAAGGGCACGCAATCTATTACAGGAAGCGGATCGCG
TACTCGTATTCGGCGGCCGTTTATTGTCAAAACGGGTGATCGGTTATCTTGCCGAGCAAAATTGGCACAGCTACTGGCAA
GTGTTACCCGAGCAAGACAGGCTAGATCCGAGTCACAATGCGAAGCACATTTGGCATGCTAATGCAGAGCAGTTTGCGGC
CTTGAACTGGTATCGTTCATCCTCAGCGAACTGGGCGAATACCTTAATCACCTATAACGATGAATTGCATAACTTATTTG
TGCGTAATATCGATCAAGGGGAATTTGGTGAAGCGCAGGTGATACGCGCTATCGCCAACACTCGTCCCTTAGAACAGCAG
CTTTTTATCGGCAACAGCCTGCCTGTACGTTTGTACGACATGTATGCCCCTGTCAGTTGCTGCACAGCGACCACTTATAC
CAACCGTGGCGCCTCGGGTATTGACGGCTTGCTCGCCACCGCCTGCGGCATTGCGGCCCACAAAGGCAAAGCCACCAGTT
TGATCATCGGTGATTTATCGCAACTGCACGATCTTAACTCGCTGGCCATCGCCAAAGGCTTAACGAGCCCGCTGGTGATC
GTGATCCTCAATAACGATGGCGGCAATATCTTTAACTTATTGCCGGTTCCCAATGAACAAGTCCGCAGTGATTATTATCG
ACTCAGCCATGGCTTAGAATTTGGCTACGCCGCAGCCATGTTTAATCTGCCCTATAATCAAGTGGATAATTTAGCCGATT
TTCAAGATAGTTATAATGAAGCGCTGGATTTTCAGGGGGCTTCGATCATTGAAGTCAATGTGAGCCAAAACCAAGCCAGC
GACCAAATCGCCGCGCTCAACCTTTGGGTGAAACAAAGCTAA

Upstream 100 bases:

>100_bases
CAGCCCCCATACAAACCCGCCGAGAATTGTTAAGCTAAGCATACATCTTGGGGCATAAACACGTTACACTAAGCCCATCT
TTATCCTAATAGATTCAGCT

Downstream 100 bases:

>100_bases
TGCCAACCATGGCCCGTTACGGCGATCCTTCACTACCGACCTTAGTGCTACTACATGGCTTTTTAGGCACTAAGGCCGAC
TGGTTGCCGTTAATGCCTGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRTENTATLNLIWGALILEELARLGVQHVCMAPGSRSTPLTLAAAQQTKLQRHLHFDERGLGFMALGLAKASRAPVAIIT
TSGTAVANLYPAIVEAWLTHVPLIVLSGDRPPELLGCGANQAIVQPGIFANYATQVNLPTPDAHIAPQALLTTVDEAVAN
QTRPVHINCMYREPLYPSELSGVILDTESPYLKPLQTWLQLARPYTQYGKCKQLSSPSDDAIMRFVHGKGVIVVGTLTPE
QDPQQLIALSQKIGWPLLTDAQSQLRQHPAAIGNIDQLLQHPRARNLLQEADRVLVFGGRLLSKRVIGYLAEQNWHSYWQ
VLPEQDRLDPSHNAKHIWHANAEQFAALNWYRSSSANWANTLITYNDELHNLFVRNIDQGEFGEAQVIRAIANTRPLEQQ
LFIGNSLPVRLYDMYAPVSCCTATTYTNRGASGIDGLLATACGIAAHKGKATSLIIGDLSQLHDLNSLAIAKGLTSPLVI
VILNNDGGNIFNLLPVPNEQVRSDYYRLSHGLEFGYAAAMFNLPYNQVDNLADFQDSYNEALDFQGASIIEVNVSQNQAS
DQIAALNLWVKQS

Sequences:

>Translated_573_residues
MRTENTATLNLIWGALILEELARLGVQHVCMAPGSRSTPLTLAAAQQTKLQRHLHFDERGLGFMALGLAKASRAPVAIIT
TSGTAVANLYPAIVEAWLTHVPLIVLSGDRPPELLGCGANQAIVQPGIFANYATQVNLPTPDAHIAPQALLTTVDEAVAN
QTRPVHINCMYREPLYPSELSGVILDTESPYLKPLQTWLQLARPYTQYGKCKQLSSPSDDAIMRFVHGKGVIVVGTLTPE
QDPQQLIALSQKIGWPLLTDAQSQLRQHPAAIGNIDQLLQHPRARNLLQEADRVLVFGGRLLSKRVIGYLAEQNWHSYWQ
VLPEQDRLDPSHNAKHIWHANAEQFAALNWYRSSSANWANTLITYNDELHNLFVRNIDQGEFGEAQVIRAIANTRPLEQQ
LFIGNSLPVRLYDMYAPVSCCTATTYTNRGASGIDGLLATACGIAAHKGKATSLIIGDLSQLHDLNSLAIAKGLTSPLVI
VILNNDGGNIFNLLPVPNEQVRSDYYRLSHGLEFGYAAAMFNLPYNQVDNLADFQDSYNEALDFQGASIIEVNVSQNQAS
DQIAALNLWVKQS
>Mature_573_residues
MRTENTATLNLIWGALILEELARLGVQHVCMAPGSRSTPLTLAAAQQTKLQRHLHFDERGLGFMALGLAKASRAPVAIIT
TSGTAVANLYPAIVEAWLTHVPLIVLSGDRPPELLGCGANQAIVQPGIFANYATQVNLPTPDAHIAPQALLTTVDEAVAN
QTRPVHINCMYREPLYPSELSGVILDTESPYLKPLQTWLQLARPYTQYGKCKQLSSPSDDAIMRFVHGKGVIVVGTLTPE
QDPQQLIALSQKIGWPLLTDAQSQLRQHPAAIGNIDQLLQHPRARNLLQEADRVLVFGGRLLSKRVIGYLAEQNWHSYWQ
VLPEQDRLDPSHNAKHIWHANAEQFAALNWYRSSSANWANTLITYNDELHNLFVRNIDQGEFGEAQVIRAIANTRPLEQQ
LFIGNSLPVRLYDMYAPVSCCTATTYTNRGASGIDGLLATACGIAAHKGKATSLIIGDLSQLHDLNSLAIAKGLTSPLVI
VILNNDGGNIFNLLPVPNEQVRSDYYRLSHGLEFGYAAAMFNLPYNQVDNLADFQDSYNEALDFQGASIIEVNVSQNQAS
DQIAALNLWVKQS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=565, Percent_Identity=40.1769911504425, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1790104, Length=309, Percent_Identity=26.2135922330097, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 62961; Mature: 62961

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTENTATLNLIWGALILEELARLGVQHVCMAPGSRSTPLTLAAAQQTKLQRHLHFDERG
CCCCCCEEHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCCC
LGFMALGLAKASRAPVAIITTSGTAVANLYPAIVEAWLTHVPLIVLSGDRPPELLGCGAN
CCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHEECCCC
QAIVQPGIFANYATQVNLPTPDAHIAPQALLTTVDEAVANQTRPVHINCMYREPLYPSEL
CCEECCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
SGVILDTESPYLKPLQTWLQLARPYTQYGKCKQLSSPSDDAIMRFVHGKGVIVVGTLTPE
CEEEEECCCCHHHHHHHHHHHHCCHHHHCCHHCCCCCCHHHHHHEECCCCEEEEEECCCC
QDPQQLIALSQKIGWPLLTDAQSQLRQHPAAIGNIDQLLQHPRARNLLQEADRVLVFGGR
CCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHCCCHHHHHCCHHHHHHHHHCCEEEEECHH
LLSKRVIGYLAEQNWHSYWQVLPEQDRLDPSHNAKHIWHANAEQFAALNWYRSSSANWAN
HHHHHHHHHHHHCCHHHHHHCCCCHHCCCCCCCCCEEEECCHHHHHEEEEECCCCCCCCC
TLITYNDELHNLFVRNIDQGEFGEAQVIRAIANTRPLEQQLFIGNSLPVRLYDMYAPVSC
EEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHEECCCCCEEEEEHHCCHHH
CTATTYTNRGASGIDGLLATACGIAAHKGKATSLIIGDLSQLHDLNSLAIAKGLTSPLVI
HEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCHHHHHHHCCCCCEEE
VILNNDGGNIFNLLPVPNEQVRSDYYRLSHGLEFGYAAAMFNLPYNQVDNLADFQDSYNE
EEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ALDFQGASIIEVNVSQNQASDQIAALNLWVKQS
HHCCCCCEEEEEECCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRTENTATLNLIWGALILEELARLGVQHVCMAPGSRSTPLTLAAAQQTKLQRHLHFDERG
CCCCCCEEHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCCC
LGFMALGLAKASRAPVAIITTSGTAVANLYPAIVEAWLTHVPLIVLSGDRPPELLGCGAN
CCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHEECCCC
QAIVQPGIFANYATQVNLPTPDAHIAPQALLTTVDEAVANQTRPVHINCMYREPLYPSEL
CCEECCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
SGVILDTESPYLKPLQTWLQLARPYTQYGKCKQLSSPSDDAIMRFVHGKGVIVVGTLTPE
CEEEEECCCCHHHHHHHHHHHHCCHHHHCCHHCCCCCCHHHHHHEECCCCEEEEEECCCC
QDPQQLIALSQKIGWPLLTDAQSQLRQHPAAIGNIDQLLQHPRARNLLQEADRVLVFGGR
CCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHCCCHHHHHCCHHHHHHHHHCCEEEEECHH
LLSKRVIGYLAEQNWHSYWQVLPEQDRLDPSHNAKHIWHANAEQFAALNWYRSSSANWAN
HHHHHHHHHHHHCCHHHHHHCCCCHHCCCCCCCCCEEEECCHHHHHEEEEECCCCCCCCC
TLITYNDELHNLFVRNIDQGEFGEAQVIRAIANTRPLEQQLFIGNSLPVRLYDMYAPVSC
EEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHEECCCCCEEEEEHHCCHHH
CTATTYTNRGASGIDGLLATACGIAAHKGKATSLIIGDLSQLHDLNSLAIAKGLTSPLVI
HEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCHHHHHHHCCCCCEEE
VILNNDGGNIFNLLPVPNEQVRSDYYRLSHGLEFGYAAAMFNLPYNQVDNLADFQDSYNE
EEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ALDFQGASIIEVNVSQNQASDQIAALNLWVKQS
HHCCCCCEEEEEECCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA