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The map label for this gene is nrfB [H]

Identifier: 160877387

GI number: 160877387

Start: 5065191

End: 5065652

Strand: Reverse

Name: nrfB [H]

Synonym: Sbal195_4285

Alternate gene names: 160877387

Gene position: 5065652-5065191 (Counterclockwise)

Preceding gene: 160877388

Following gene: 160877386

Centisome position: 94.73

GC content: 49.13

Gene sequence:

>462_bases
ATGTCCAAAAGACAATGGCTGTGGTCGATTCGCTTGGGTGCAATTGCATTGCTTTTAAGCTTTGGTGCTACAAGTGCCGA
GTCGGCCCTTCAAATTAACAGTGACACGACTTGTATGAAGTGCCACAAACGTAACGGTCAAATGTTAGGGCATCACGGCC
AAGATGCGATGAAAATGACCTGTTCAACCTGTCATGGCGAAAAAGGCGACCACCCTAAAAAGCCGAATGATATCGTGGTG
TTTGGTATGAAAGATGGCTCGGAGCTTGATACTCAGCTCGCCGTTTGTCACAAGTGCCATACCCAGCGAAAACTGGGCGA
AACCGAATGGACGCATAATGTGCACGCACAAAAAGTGCCCTGTGCCGCCTGTCATAAATTACATAGCCCAACCGAACCTA
TGGCGGATATGACGCCAAAAGTTCGCAGTGAGCTGTGCCGCAATTGCCATAGTGGCCAGTAG

Upstream 100 bases:

>100_bases
TAAAGCAAGCGTTAGCCGTCTGATCCGACCGTCGGGTTCGGGTAACTCCGAACCCTGACACTCCTCTTCTCCCTTCCCAA
AATAAATGTGAGGACTCCCT

Downstream 100 bases:

>100_bases
GGGTAGGTTATGGAAAATTCAAAAAGAAGATTTCTAAAAGGCACATGTGCCTTATTTGCTGGCGCTACGGGCGCATCACT
GCTCGCCACAGTGAATGCCG

Product: formate-dependent nitrite reductase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSKRQWLWSIRLGAIALLLSFGATSAESALQINSDTTCMKCHKRNGQMLGHHGQDAMKMTCSTCHGEKGDHPKKPNDIVV
FGMKDGSELDTQLAVCHKCHTQRKLGETEWTHNVHAQKVPCAACHKLHSPTEPMADMTPKVRSELCRNCHSGQ

Sequences:

>Translated_153_residues
MSKRQWLWSIRLGAIALLLSFGATSAESALQINSDTTCMKCHKRNGQMLGHHGQDAMKMTCSTCHGEKGDHPKKPNDIVV
FGMKDGSELDTQLAVCHKCHTQRKLGETEWTHNVHAQKVPCAACHKLHSPTEPMADMTPKVRSELCRNCHSGQ
>Mature_152_residues
SKRQWLWSIRLGAIALLLSFGATSAESALQINSDTTCMKCHKRNGQMLGHHGQDAMKMTCSTCHGEKGDHPKKPNDIVVF
GMKDGSELDTQLAVCHKCHTQRKLGETEWTHNVHAQKVPCAACHKLHSPTEPMADMTPKVRSELCRNCHSGQ

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=173, Percent_Identity=31.2138728323699, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16974; Mature: 16843

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
11.8 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
11.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRQWLWSIRLGAIALLLSFGATSAESALQINSDTTCMKCHKRNGQMLGHHGQDAMKMT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHH
CSTCHGEKGDHPKKPNDIVVFGMKDGSELDTQLAVCHKCHTQRKLGETEWTHNVHAQKVP
HHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CAACHKLHSPTEPMADMTPKVRSELCRNCHSGQ
HHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKRQWLWSIRLGAIALLLSFGATSAESALQINSDTTCMKCHKRNGQMLGHHGQDAMKMT
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHH
CSTCHGEKGDHPKKPNDIVVFGMKDGSELDTQLAVCHKCHTQRKLGETEWTHNVHAQKVP
HHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CAACHKLHSPTEPMADMTPKVRSELCRNCHSGQ
HHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]