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The map label for this gene is nrfD [H]

Identifier: 160877385

GI number: 160877385

Start: 5063557

End: 5064498

Strand: Reverse

Name: nrfD [H]

Synonym: Sbal195_4283

Alternate gene names: 160877385

Gene position: 5064498-5063557 (Counterclockwise)

Preceding gene: 160877386

Following gene: 160877383

Centisome position: 94.71

GC content: 46.6

Gene sequence:

>942_bases
ATGAGTGCCCTACATTTTGATACCTTAGTCTGGCATTGGCCGATTGCGATCTACCTGTTTTTGGCGGGTGTATCAGCAGG
GGCTATGTTCTTCGCCGTACTGTTGAAACATTTTGTTTTGAAGCAAAACGCCTATCAGTCTGGCTTTGTGCGAGCCGCCT
GTATTGTCGCCCCTGTCGCCGTGATGGCAGGCTTAGGCATATTGGTTGCAGACTTAACTAAACCCCTCGATTTCTGGAAG
ATTTTGGTGTTCTACAATCCTAAATCTGTGATGTCGATGGGCGTACTGATTCTGCTGATTTACCAAGTGTTTATGTTTAT
GTGGATTGGGTTAACTTTCCGCAAACCTGTCGATGCTTGGTGTGAATCGCGTTTCCCTATCGTCCTTAAGCTGACGGCTT
TGCTAGCTCGGTTTGAGGCGACGATCACGGGATTACTGGTGATTCTATCCTTAGCATTGGGCGCTTATACCGGCTTCTTG
TTATCGGCGTTACCTGGTTATCCCATGCTGAAAAATCCTGTGCTGCCTTTACTGTTCTTGGCATCTGGGCTGTCTTCCGG
CGCGGCATCTTCTTTACTCGGTGGCGTGTTGATGAATGGCAAACCGAATGACAAAGAGGTGCACTTTATCCACAAGGTTG
AAATTCCACTAATTTTAGTCGAAATCGTGCTGATTTTTACCTTCTTTATGGGCTTAGTACTAACAGGTGGACAGAGTAAA
ATTGCCGCCTTTAATGCTTTAGGTTATGGCTTCTGGGGCTGGATTTTCTGGGTAGGGATTATCGGACTCGGTTTATCAGG
CCCGTTAGCGATGAACCTGTTTATGACTACGACGTCGAAACGCAAATGGGCTTATGTAGCAGGGACGGCTTGCTTGAGCT
TAATCGGGGTGTTATTGCTACGTAACTTCATACTGTACACAGGACAAATGACGATCGCTTAA

Upstream 100 bases:

>100_bases
TACAAGTGCTGCGCGCTAATCCAACTCAACGCGCGAAAGTCGCCCTCGGAACGCGACCTAAGTTGTTCCGTATCCTGGCG
AAATCCGGGGAGGTGATACT

Downstream 100 bases:

>100_bases
GGTGCGAACGCTTCATTCAAGACGAACAAGATGATAATAATCAAGGAAATCGACGGATCGCGGAAACAATGTCAAAGGAA
AACATTGTAGATGAGGCCCT

Product: cytochrome c nitrite reductase, NrfD subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSALHFDTLVWHWPIAIYLFLAGVSAGAMFFAVLLKHFVLKQNAYQSGFVRAACIVAPVAVMAGLGILVADLTKPLDFWK
ILVFYNPKSVMSMGVLILLIYQVFMFMWIGLTFRKPVDAWCESRFPIVLKLTALLARFEATITGLLVILSLALGAYTGFL
LSALPGYPMLKNPVLPLLFLASGLSSGAASSLLGGVLMNGKPNDKEVHFIHKVEIPLILVEIVLIFTFFMGLVLTGGQSK
IAAFNALGYGFWGWIFWVGIIGLGLSGPLAMNLFMTTTSKRKWAYVAGTACLSLIGVLLLRNFILYTGQMTIA

Sequences:

>Translated_313_residues
MSALHFDTLVWHWPIAIYLFLAGVSAGAMFFAVLLKHFVLKQNAYQSGFVRAACIVAPVAVMAGLGILVADLTKPLDFWK
ILVFYNPKSVMSMGVLILLIYQVFMFMWIGLTFRKPVDAWCESRFPIVLKLTALLARFEATITGLLVILSLALGAYTGFL
LSALPGYPMLKNPVLPLLFLASGLSSGAASSLLGGVLMNGKPNDKEVHFIHKVEIPLILVEIVLIFTFFMGLVLTGGQSK
IAAFNALGYGFWGWIFWVGIIGLGLSGPLAMNLFMTTTSKRKWAYVAGTACLSLIGVLLLRNFILYTGQMTIA
>Mature_312_residues
SALHFDTLVWHWPIAIYLFLAGVSAGAMFFAVLLKHFVLKQNAYQSGFVRAACIVAPVAVMAGLGILVADLTKPLDFWKI
LVFYNPKSVMSMGVLILLIYQVFMFMWIGLTFRKPVDAWCESRFPIVLKLTALLARFEATITGLLVILSLALGAYTGFLL
SALPGYPMLKNPVLPLLFLASGLSSGAASSLLGGVLMNGKPNDKEVHFIHKVEIPLILVEIVLIFTFFMGLVLTGGQSKI
AAFNALGYGFWGWIFWVGIIGLGLSGPLAMNLFMTTTSKRKWAYVAGTACLSLIGVLLLRNFILYTGQMTIA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=317, Percent_Identity=45.7413249211356, Blast_Score=244, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 34197; Mature: 34066

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALHFDTLVWHWPIAIYLFLAGVSAGAMFFAVLLKHFVLKQNAYQSGFVRAACIVAPVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VMAGLGILVADLTKPLDFWKILVFYNPKSVMSMGVLILLIYQVFMFMWIGLTFRKPVDAW
HHHHHHHHHHHHHCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHH
CESRFPIVLKLTALLARFEATITGLLVILSLALGAYTGFLLSALPGYPMLKNPVLPLLFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ASGLSSGAASSLLGGVLMNGKPNDKEVHFIHKVEIPLILVEIVLIFTFFMGLVLTGGQSK
HHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHH
IAAFNALGYGFWGWIFWVGIIGLGLSGPLAMNLFMTTTSKRKWAYVAGTACLSLIGVLLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEHHHHHHHHHHHHHHHH
RNFILYTGQMTIA
HHHHHHCCCEECC
>Mature Secondary Structure 
SALHFDTLVWHWPIAIYLFLAGVSAGAMFFAVLLKHFVLKQNAYQSGFVRAACIVAPVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VMAGLGILVADLTKPLDFWKILVFYNPKSVMSMGVLILLIYQVFMFMWIGLTFRKPVDAW
HHHHHHHHHHHHHCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHH
CESRFPIVLKLTALLARFEATITGLLVILSLALGAYTGFLLSALPGYPMLKNPVLPLLFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ASGLSSGAASSLLGGVLMNGKPNDKEVHFIHKVEIPLILVEIVLIFTFFMGLVLTGGQSK
HHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHH
IAAFNALGYGFWGWIFWVGIIGLGLSGPLAMNLFMTTTSKRKWAYVAGTACLSLIGVLLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCEEHHHHHHHHHHHHHHHH
RNFILYTGQMTIA
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]