Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 160877243

GI number: 160877243

Start: 4900997

End: 4901875

Strand: Direct

Name: prpB [H]

Synonym: Sbal195_4140

Alternate gene names: 160877243

Gene position: 4900997-4901875 (Clockwise)

Preceding gene: 160877242

Following gene: 160877244

Centisome position: 91.65

GC content: 50.4

Gene sequence:

>879_bases
ATGACCCAAAGCGCAGGATTACGTTTTCGCCAAGCTCTTGCTAAGGCCAAACCGTTACAAATCGTTGGCACCACCAATGC
CTATTTTGCCTTAATGGCAGAACAAACAGGCTTCCAAGCCTTGTATCTTTCGGGCGCTGGCGTTGCCAATGCTTCCTACG
GTTTGCCTGACTTGGGCATGACCTCAATGAATGACGTGCTGATTGATGCAGGCCGCATCACCTCTGCCACGCAAGTGCCT
TTATTGGTCGATATCGATACTGGCTGGGGCGGCGCATTCAACATTGCGCGTACCATCAAAGAATTCGAAAAAATCGGTGT
CGCCGCAGTGCACATGGAAGACCAAGTGTCGCAAAAGCGTTGCGGTCACAGACCCAACAAAGCAGTTGTGAGCATAGAAG
AAATGGTCGATCGCATTAAAGCGGCCGTTGATGCCCGTACCGATCCTAACTTTGTGATCATGGCGCGTACCGATGCGGTT
GCCGTGGAAGGCTTAGAGGCAGGTATCGAACGCGCGCAAGCCTATATTGCCGCCGGCGCTGACATGATCTTCGCCGAAGC
TTTAACTGAGCTAGACCAATATCGCCACTTTAAAGCGCAAGTCAAAGCACCGATTTTGGCCAACATGACTGAATTTGGTC
AAACCCAATTGTTCAATAAAGAAGAACTCGCCGAGGCGGGCGCCGACATGGTGCTTTATCCACTCGGTACTTTTCGTGCA
GCCAACCAAGCGGCACTGAACGTCATGCAAGCGCTGATGAATGATGGTCATCAACGCAACGTGCTCGACACTATGCAGAC
CCGTAAAGACTTGTATAAGTACTTGGGCTACCACGCCTTTGAAGACAAGTTAGATCAACTGTTTAGCCAAGATAAATAG

Upstream 100 bases:

>100_bases
TTGCAAGCCAATGCAGCCGCAAAAGAAACCTTAGCCAACATTAGCTAAGTTCATACGGCTCAATATGCCCACTTCCCATA
AAATGGAAAAGGATAACACC

Downstream 100 bases:

>100_bases
CTAAACAAGCGAGAAAGAAAAATACACAAGACCATGGATTGTCAGTAGAAGCGCAAGCTCATGTTGTTACTGAAACCCAG
ACGCTTTGCCCCAAGCAGCA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVP
LLVDIDTGWGGAFNIARTIKEFEKIGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAV
AVEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAEAGADMVLYPLGTFRA
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK

Sequences:

>Translated_292_residues
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVP
LLVDIDTGWGGAFNIARTIKEFEKIGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAV
AVEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAEAGADMVLYPLGTFRA
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
>Mature_291_residues
TQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVPL
LVDIDTGWGGAFNIARTIKEFEKIGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVA
VEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAEAGADMVLYPLGTFRAA
NQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=60.1374570446735, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1790445, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320908, Length=222, Percent_Identity=27.9279279279279, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31949; Mature: 31818

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGM
CCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQVPLLVDIDTGWGGAFNIARTIKEFEKIGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVAVEGLEAGIERAQAYIAAGA
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCH
DMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAEAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCHHHH
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGM
CCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQVPLLVDIDTGWGGAFNIARTIKEFEKIGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVAVEGLEAGIERAQAYIAAGA
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCH
DMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAEAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCHHHH
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]