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The map label for this gene is amiB [H]

Identifier: 160877000

GI number: 160877000

Start: 4590730

End: 4592151

Strand: Reverse

Name: amiB [H]

Synonym: Sbal195_3896

Alternate gene names: 160877000

Gene position: 4592151-4590730 (Counterclockwise)

Preceding gene: 160877001

Following gene: 160876999

Centisome position: 85.88

GC content: 47.4

Gene sequence:

>1422_bases
ATGATAAAAAATAACCCTTATTTTCAAATTATTATTCTTTCTTTATGTTCTTTTTTATCTGTGGCGGCCCATGCGGCTAA
TCAGTTAGAAAGCGTACGCATTTGGGCTGCGCCTGAGTCGACTCGAATCGTGTTTGACTTAAGCGAAGCGCCTGATTACA
CCTATTTCTCTTTAGATGGCCCTAATCGCTTAGTGGTGGATTTAAAAAAGTCCGCCACTAAAGTTGCGCTTAAAAATCTT
GAAAACAACAGTAAGTTAGTCAAAGGCGTGCGAGTCAGTAAGTCGCCGACTAAGGGTGATTTGCGTTTAGTCATCGATTT
GGTGAAACCACTGAATGCGAGCTTGTTCTCGTTGCCCGTGACCGCGCCCTATGGCAATCGTCTCGTGGTTGATTTAGAAG
ATAAAACCTTAACAACCGCCACCGCAGTTGTCTCATCGACACCCGTTAAAACCGTTACCCAAGCGGCGCAATCATCCCGC
GATATCGTGATTGCTATCGATGCGGGTCATGGCGGCGACGACCCCGGCTCCATTGGCCCTTCTGGCGTTTATGAAAAAAA
GGTTGCCCTAGAAATCGCCAGACGTGTTTCGAGTAAAATCAATGACACGCCCGGCATGCGCGCTGTGATGATCCGCACCG
GCGATTATTTTGTGAATTTGAATAAGCGCTCTGAGTTGGCCCGTAACAGTAAGGCCGATCTGCTGATTTCTATCCATGCC
GATGCTTTTACTTCCCCAAATCCTCGCGGCGCCTCTGTGTGGGTATTATCCATGCGTCGGGCTAACAGTGAAATTGGCCG
TTGGTTAGAGCAAAAAGAAAAGCATTCTGAGTTACTTGGCGGCGCGGGTGAGATTATCCAAAACACGGATAATGAACAGT
ATTTAGCCATGACGCTGCTCGATATGTCGATGAACAGTTCGATGGCCATTGGTCATTCAGTCGCTGGCGATATTCTTAAG
GATTTAGGCGGTGTGACTGATTTACATAAGAGTCGCCCCGAGTCGGCGAGTTTAGCTGTGCTGAAATCACCGGATATTCC
TTCAATCTTGGTGGAAACGGGATTTATCTCGAATCCTAAAGAAGAGCGTTTGCTGTCGAGTAGTCGTCATCAAGAAAACA
TCGCTAATGCTATCTACAAGGGTGTGAGTCGTTACTATCATAATAATCCGCCAGCGGATACCTTGCTTGCCCAGCGAGGC
GGCGCTAGCTCTAGCAGTGCTAAATCGACTAAAGAGAGAACGCCAACCGCTTATGTGGGGAGTTCAACGGCATCGAACGT
CAAACATAAAGTCAGTCGTGGCGAGTCTTTATCGGCTATCGCGCAGCGTTATCGTGTGCCTATGTCTAGCATCAAACAAG
CGAATGGGATGAAGTCCGATGTAGTGCAGTTAGGGCAAACATTAGTCATACCCCAGAGTTAA

Upstream 100 bases:

>100_bases
ATATGCATTTAAGTTATCAAAATAGTGGGCGTGAAATACGCATAGAGGCCTTGTCAGAGCCAGGTGAAAAGTTATTAAAA
GCGATAAAATAAAAACTAAA

Downstream 100 bases:

>100_bases
ATAGGAAAGCATGATGGGCATTCAGATCCTGCCACCGCAATTAGCCAACCAAATTGCGGCGGGGGAAGTGGTTGAGAGAC
CCGCTTCCGTAGTCAAAGAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIKNNPYFQIIILSLCSFLSVAAHAANQLESVRIWAAPESTRIVFDLSEAPDYTYFSLDGPNRLVVDLKKSATKVALKNL
ENNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNASLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQAAQSSR
DIVIAIDAGHGGDDPGSIGPSGVYEKKVALEIARRVSSKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK
DLGGVTDLHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLSSSRHQENIANAIYKGVSRYYHNNPPADTLLAQRG
GASSSSAKSTKERTPTAYVGSSTASNVKHKVSRGESLSAIAQRYRVPMSSIKQANGMKSDVVQLGQTLVIPQS

Sequences:

>Translated_473_residues
MIKNNPYFQIIILSLCSFLSVAAHAANQLESVRIWAAPESTRIVFDLSEAPDYTYFSLDGPNRLVVDLKKSATKVALKNL
ENNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNASLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQAAQSSR
DIVIAIDAGHGGDDPGSIGPSGVYEKKVALEIARRVSSKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK
DLGGVTDLHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLSSSRHQENIANAIYKGVSRYYHNNPPADTLLAQRG
GASSSSAKSTKERTPTAYVGSSTASNVKHKVSRGESLSAIAQRYRVPMSSIKQANGMKSDVVQLGQTLVIPQS
>Mature_473_residues
MIKNNPYFQIIILSLCSFLSVAAHAANQLESVRIWAAPESTRIVFDLSEAPDYTYFSLDGPNRLVVDLKKSATKVALKNL
ENNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNASLFSLPVTAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQAAQSSR
DIVIAIDAGHGGDDPGSIGPSGVYEKKVALEIARRVSSKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK
DLGGVTDLHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLSSSRHQENIANAIYKGVSRYYHNNPPADTLLAQRG
GASSSSAKSTKERTPTAYVGSSTASNVKHKVSRGESLSAIAQRYRVPMSSIKQANGMKSDVVQLGQTLVIPQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=438, Percent_Identity=38.3561643835616, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082163, Length=406, Percent_Identity=34.7290640394089, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=40.5982905982906, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 51052; Mature: 51052

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNNPYFQIIILSLCSFLSVAAHAANQLESVRIWAAPESTRIVFDLSEAPDYTYFSLDG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKSATKVALKNLENNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNASLFSLPV
CCEEEEEEHHHHHHHHHHHCCCCCHHHHCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEE
TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQAAQSSRDIVIAIDAGHGGDDPGSIGP
ECCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
SGVYEKKVALEIARRVSSKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL
CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE
DMSMNSSMAIGHSVAGDILKDLGGVTDLHKSRPESASLAVLKSPDIPSILVETGFISNPK
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH
EERLLSSSRHQENIANAIYKGVSRYYHNNPPADTLLAQRGGASSSSAKSTKERTPTAYVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCEEEC
SSTASNVKHKVSRGESLSAIAQRYRVPMSSIKQANGMKSDVVQLGQTLVIPQS
CCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCEEECCCC
>Mature Secondary Structure
MIKNNPYFQIIILSLCSFLSVAAHAANQLESVRIWAAPESTRIVFDLSEAPDYTYFSLDG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKSATKVALKNLENNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNASLFSLPV
CCEEEEEEHHHHHHHHHHHCCCCCHHHHCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEE
TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQAAQSSRDIVIAIDAGHGGDDPGSIGP
ECCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
SGVYEKKVALEIARRVSSKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC
DAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL
CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE
DMSMNSSMAIGHSVAGDILKDLGGVTDLHKSRPESASLAVLKSPDIPSILVETGFISNPK
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH
EERLLSSSRHQENIANAIYKGVSRYYHNNPPADTLLAQRGGASSSSAKSTKERTPTAYVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCEEEC
SSTASNVKHKVSRGESLSAIAQRYRVPMSSIKQANGMKSDVVQLGQTLVIPQS
CCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]