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The map label for this gene is rhtB [H]

Identifier: 160875843

GI number: 160875843

Start: 3237816

End: 3238505

Strand: Direct

Name: rhtB [H]

Synonym: Sbal195_2731

Alternate gene names: 160875843

Gene position: 3237816-3238505 (Clockwise)

Preceding gene: 160875841

Following gene: 160875845

Centisome position: 60.55

GC content: 52.75

Gene sequence:

>690_bases
TTGGCTAAACTCGCGGTTTTCATTACACTATCGGCCTTCCCATCTTCACCCAAGACAGACCAAACCATGCCAGATTTTGC
CTTACTCGCAGTGTTTATCCCGACCTTCTTCTTTGTTTCTATCACCCCGGGCATGTGCATGACGCTCGCCATGACCTTGG
GCATGAGCATAGGTGTGCGCCGCACCCTGTGGATGATGCTGGGTGAACTGGTAGGGGTGGCGTTAGTGGCGACAGCCGCC
GTGATGGGCGTTGCCAGTATCATGCTCAACTATCCCGAAGTCTTTCAAGTCTTTAAATGGGTTGGCGGAATTTATTTAGG
CTTTATCGGCGTACAAATGTGGCGCGCCCGCGGTAAGATGGCCATGCTAAGCGATGACGACACTAACCCCAGAGCCAGCA
ATGTTACCTTGATCTCCCAAGGCTTTATTACCGCCATTGCCAATCCTAAAGGTTGGGCATTTATGGTGTCGCTACTACCA
CCTTTTATCAATGCCGAGCGCGAAGTTGCGCCGCAGCTGCTCGCACTGCTGAGCATTATCATGGTGACCGAGTTTACCTC
TATGCTCGCCTACGCCAGCGGCGGCAAAAGCCTGCGTATTTTCCTAAGCCGCGGCGATAATATTCGCTGGTTAAACCGCA
TTGCAGGCAGCCTGATGATAGGCGTGGGCGTGTGGTTAGCCATGAGCTAA

Upstream 100 bases:

>100_bases
AATAGCAAGGTCTTGTGGTGATAACAGAGTAAACATCCCAAAAAGATGAATTGTTTACATACTAAGTTACAGTTACTCAT
CGAAAAAAACGGGACGCACA

Downstream 100 bases:

>100_bases
CATTCATAGCTAACCGTTTGAATTAACAGCTTAGATAAAACTACTTAGGTTGTACTACATAGGTTGAACATGCACGACAT
AAATGAGAGCCCAATAAAGC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MAKLAVFITLSAFPSSPKTDQTMPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELVGVALVATAA
VMGVASIMLNYPEVFQVFKWVGGIYLGFIGVQMWRARGKMAMLSDDDTNPRASNVTLISQGFITAIANPKGWAFMVSLLP
PFINAEREVAPQLLALLSIIMVTEFTSMLAYASGGKSLRIFLSRGDNIRWLNRIAGSLMIGVGVWLAMS

Sequences:

>Translated_229_residues
MAKLAVFITLSAFPSSPKTDQTMPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELVGVALVATAA
VMGVASIMLNYPEVFQVFKWVGGIYLGFIGVQMWRARGKMAMLSDDDTNPRASNVTLISQGFITAIANPKGWAFMVSLLP
PFINAEREVAPQLLALLSIIMVTEFTSMLAYASGGKSLRIFLSRGDNIRWLNRIAGSLMIGVGVWLAMS
>Mature_228_residues
AKLAVFITLSAFPSSPKTDQTMPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVRRTLWMMLGELVGVALVATAAV
MGVASIMLNYPEVFQVFKWVGGIYLGFIGVQMWRARGKMAMLSDDDTNPRASNVTLISQGFITAIANPKGWAFMVSLLPP
FINAEREVAPQLLALLSIIMVTEFTSMLAYASGGKSLRIFLSRGDNIRWLNRIAGSLMIGVGVWLAMS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=196, Percent_Identity=27.0408163265306, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 24907; Mature: 24776

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
7.9 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
7.5 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLAVFITLSAFPSSPKTDQTMPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVR
CCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
RTLWMMLGELVGVALVATAAVMGVASIMLNYPEVFQVFKWVGGIYLGFIGVQMWRARGKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AMLSDDDTNPRASNVTLISQGFITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSII
EEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHCHHCCCHHHHHHHHHHHHHHH
MVTEFTSMLAYASGGKSLRIFLSRGDNIRWLNRIAGSLMIGVGVWLAMS
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKLAVFITLSAFPSSPKTDQTMPDFALLAVFIPTFFFVSITPGMCMTLAMTLGMSIGVR
CEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
RTLWMMLGELVGVALVATAAVMGVASIMLNYPEVFQVFKWVGGIYLGFIGVQMWRARGKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AMLSDDDTNPRASNVTLISQGFITAIANPKGWAFMVSLLPPFINAEREVAPQLLALLSII
EEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHCHHCCCHHHHHHHHHHHHHHH
MVTEFTSMLAYASGGKSLRIFLSRGDNIRWLNRIAGSLMIGVGVWLAMS
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]