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The map label for this gene is puuB [H]

Identifier: 160875640

GI number: 160875640

Start: 2987703

End: 2988992

Strand: Reverse

Name: puuB [H]

Synonym: Sbal195_2528

Alternate gene names: 160875640

Gene position: 2988992-2987703 (Counterclockwise)

Preceding gene: 160875642

Following gene: 160875638

Centisome position: 55.9

GC content: 51.32

Gene sequence:

>1290_bases
ATGCCAGCAGAGCGTTGCGCCTCCTATTACAATGCCACCATAAACCAAGAGTCGAATTATCCTCAGTTAGAAGGTGACAT
TCGCGTCGATGTGGTGATTGTCGGTGGCGGATTTACCGGTGTAGCAACGGCGCTGGAATTGTCCGAGAAGGGCTACAAGG
TGGCACTTATTGAGGCTAATAAAATTGCGTGGGGCGCAACCGGACGCAACGGCGGGCAGGTGACGGGCAGTTTATCTGGC
GATGCCGCGATGACAAAACAACTGCGTCGTCAGATTGGCAATGAAGCTGAAGATTATGTGTGGAATTTACGTTGGCGCGG
TCACGACATTATTAAAAATCGGGTGGCCAAGTACGGCATAGAGTGTGATTTAAAATTTGGTCATATACAAACCGCTTACC
AGATGCAGCACATGCACGAGCTTAGGGCTATGCATGATGAAGCGCAACGTCGCGGCATGGGGGAGCTTATGACCTTAGTC
TCCGCCGATGAAATGCCCGCCTATTTAGGCTCGCCCTTGTATCACGGCGGCTTAGTCAATCGGCGCAACATGCATTTACA
CTCAGTTAATCTATGCCTGGGTGAGGCCCGCGCCGCAGCAGGTCTTGGGACGTTGATCTTTGAACACTCGCCCGTACTCG
ATATTGAGGAGGGCGATGTAGCTACCGTTGTCACCGCCAAGGGAAAAGTGCGCGCCAATAGCGTGCTGATCGCGGGGAAT
GCCTACCATAAACTTGCGCGTCCTCAACTGCGTGGCATGTTATTTCCTGCGTCCTTAGGTAATTGTGCCACGGCGATTCT
GTCCGATGAGATCGCTGCGCAAATCAATCCGCAGGATTTAGCCGTGTACGATTGTCGCTTTGTGCTGGATTACTATCGCC
TGACCGCAGATAAACGGTTGATGTTCGGCGGCGGCACTAATTACAGCGGCCGCGATCCTAAAAATGTGGCGGCGGAATTA
CGGCCTGCAATTGAGCGCACGTTTCCACAATTAAAGGGCGTTGATATCGAATTTGCGTGGGCGGGAATGGCGGGAATTGT
GATTAACCGTATCCCGCAGCTCGGCAAAGTATCGCCTAATGTATTCTATTGCCAAGGATATTCTGGGCATGGGGTGGCGA
CATCACACATTATGGCCGAGATCATGGCTAATGCGATGGACGGACAATTGCGTGAATTTGATCTGTTTGCCGGCATGCAC
CATATCCGTATTCCGTTAAATGAATGGTTTGGTAATCAAGCGTTGGCGTTAGGCATGTTGTATTACACTTTACGGGAAAA
CTGGCGCTAG

Upstream 100 bases:

>100_bases
AACACCCATTACCAGCACACAGCTTTTAGTACTTAACGTTCAACACTCAACTTTTAATATTTAACTTTCAACACTTAACT
TTCAACATCAAGGAATGACT

Downstream 100 bases:

>100_bases
TTTGCGGCGTCTTGTCTGTTATCGCTTCGTTTGTTCGAACTGAGTAATCCGCTCAACAACAGACGGCTCCACAATAAACC
TCTCAGCAATAGATTTTTTA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPAERCASYYNATINQESNYPQLEGDIRVDVVIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIECDLKFGHIQTAYQMQHMHELRAMHDEAQRRGMGELMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGTLIFEHSPVLDIEEGDVATVVTAKGKVRANSVLIAGN
AYHKLARPQLRGMLFPASLGNCATAILSDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMH
HIRIPLNEWFGNQALALGMLYYTLRENWR

Sequences:

>Translated_429_residues
MPAERCASYYNATINQESNYPQLEGDIRVDVVIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIECDLKFGHIQTAYQMQHMHELRAMHDEAQRRGMGELMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGTLIFEHSPVLDIEEGDVATVVTAKGKVRANSVLIAGN
AYHKLARPQLRGMLFPASLGNCATAILSDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMH
HIRIPLNEWFGNQALALGMLYYTLRENWR
>Mature_428_residues
PAERCASYYNATINQESNYPQLEGDIRVDVVIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSGD
AAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIECDLKFGHIQTAYQMQHMHELRAMHDEAQRRGMGELMTLVS
ADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGTLIFEHSPVLDIEEGDVATVVTAKGKVRANSVLIAGNA
YHKLARPQLRGMLFPASLGNCATAILSDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAELR
PAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMHH
IRIPLNEWFGNQALALGMLYYTLRENWR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=425, Percent_Identity=36.4705882352941, Blast_Score=291, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47320; Mature: 47189

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAERCASYYNATINQESNYPQLEGDIRVDVVIVGGGFTGVATALELSEKGYKVALIEAN
CCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHHHCCC
ECDLKFGHIQTAYQMQHMHELRAMHDEAQRRGMGELMTLVSADEMPAYLGSPLYHGGLVN
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGTLIFEHSPVLDIEEGDVATVVTAKGKVRANSVLIAGN
CCCCEEEEEEEHHHHHHHHCCCCCEEECCCCCEEECCCCEEEEEEECCCEEECEEEEECC
AYHKLARPQLRGMLFPASLGNCATAILSDEIAAQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMHHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC
>Mature Secondary Structure 
PAERCASYYNATINQESNYPQLEGDIRVDVVIVGGGFTGVATALELSEKGYKVALIEAN
CHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHHHCCC
ECDLKFGHIQTAYQMQHMHELRAMHDEAQRRGMGELMTLVSADEMPAYLGSPLYHGGLVN
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGTLIFEHSPVLDIEEGDVATVVTAKGKVRANSVLIAGN
CCCCEEEEEEEHHHHHHHHCCCCCEEECCCCCEEECCCCEEEEEEECCCEEECEEEEECC
AYHKLARPQLRGMLFPASLGNCATAILSDEIAAQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMHHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]