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The map label for this gene is ispB [H]

Identifier: 160874188

GI number: 160874188

Start: 1267297

End: 1268292

Strand: Reverse

Name: ispB [H]

Synonym: Sbal195_1068

Alternate gene names: 160874188

Gene position: 1268292-1267297 (Counterclockwise)

Preceding gene: 160874196

Following gene: 160874186

Centisome position: 23.72

GC content: 48.29

Gene sequence:

>996_bases
ATGACCCCACAACCAGAGTCTACTATGGATTTAAACGCTATTCGTCAACTGGCTGACACTGATATGCAAGCAGTCAATCA
GCTGATATATAAACAGCTTGAATCAGATGTCGCCCTAATTAATCAATTAGGTTTTTACATTATTAATGGTGGTGGTAAAC
GTCTTCGTCCTCTGCTGTCGGTATTAGCCGCACGTGCGGTGAGCTACGAAGGCGAAGCGCATCTTAAGCTAGCGGCGATT
ATTGAGTTTATCCATACGGCCTCATTGCTCCACGATGATGTAGTTGATGAATCGACATTGCGCCGCGGACGAGAAACCGC
CAACGCACTGTTCGGCAATAGCGCCAGCGTGCTGGTCGGGGATTTTCTTTATACCCGTTCATTCCAGATGATGACTGAAC
TCGACAGCATGAAAGTGCTCAGAGTGTTAGCGGATACCACGAACGTGTTGGCCGAGGGCGAAGTCTTACAACTGATGAAT
TGCAACGATCCTGACACCACAGAAGAAAGCTATATGCGGGTCATTTATTGCAAAACCGCTAAGTTGTTTGAAGCGGCCAC
TCTGCTTGCGGGTGTGTTAGCGGGTGCGACGCCAGAGCAAGAAACCGCCTTAGGTAACTATGGTAAGTATCTTGGTACCG
CCTTCCAGCTTACCGACGATTTACTCGACTATACCGCCGACAGTGATGAGCTAGGCAAGAACATAGGTGATGATCTTGCT
GAAGGTAAACCAACTCTGCCACTCATCTATGCCATTGCCCATGGCACAGACGAAGAGAAACACCTCATACGTCAAGCAAT
TGAGCAAGGCGATGGTACACAGGCCATAGATAAAATCGTGGCAGCACTGCACCATTGCGGCGCGTTAAACTACACCCAGC
AAAAAGCGATTGAAGAATCAGAGAAAGCCATCGAGGCATTATCGATTCTGCCTGACAGCGACTACAAGCAAGCCTTAGTT
GCGCTTGCTAAGATTTCGGTCGACCGTAATCACTGA

Upstream 100 bases:

>100_bases
TTAACTCTCACATTAGTTGAGCTAGTGCTGTCATATGTCAGAATTTTAGGTAAAATTCATTTCATTATAACACGTGAGCC
TAAACAGGGGCCGGTACGTA

Downstream 100 bases:

>100_bases
TCACTCGCTAATCCGTTTTTAGCCCAAAGAAAAGGTCTTACTCAGTAAGACCTTTTTTATTGCCTCAAATCCGCAATCCT
AAGATTAAACCTGCCAGCGG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTPQPESTMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVSYEGEAHLKLAAI
IEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMKVLRVLADTTNVLAEGEVLQLMN
CNDPDTTEESYMRVIYCKTAKLFEAATLLAGVLAGATPEQETALGNYGKYLGTAFQLTDDLLDYTADSDELGKNIGDDLA
EGKPTLPLIYAIAHGTDEEKHLIRQAIEQGDGTQAIDKIVAALHHCGALNYTQQKAIEESEKAIEALSILPDSDYKQALV
ALAKISVDRNH

Sequences:

>Translated_331_residues
MTPQPESTMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVSYEGEAHLKLAAI
IEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMKVLRVLADTTNVLAEGEVLQLMN
CNDPDTTEESYMRVIYCKTAKLFEAATLLAGVLAGATPEQETALGNYGKYLGTAFQLTDDLLDYTADSDELGKNIGDDLA
EGKPTLPLIYAIAHGTDEEKHLIRQAIEQGDGTQAIDKIVAALHHCGALNYTQQKAIEESEKAIEALSILPDSDYKQALV
ALAKISVDRNH
>Mature_330_residues
TPQPESTMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVSYEGEAHLKLAAII
EFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMKVLRVLADTTNVLAEGEVLQLMNC
NDPDTTEESYMRVIYCKTAKLFEAATLLAGVLAGATPEQETALGNYGKYLGTAFQLTDDLLDYTADSDELGKNIGDDLAE
GKPTLPLIYAIAHGTDEEKHLIRQAIEQGDGTQAIDKIVAALHHCGALNYTQQKAIEESEKAIEALSILPDSDYKQALVA
LAKISVDRNH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=29.5031055900621, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=25.6603773584906, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=25.6603773584906, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=63.6645962732919, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=30.2127659574468, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=34.1614906832298, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6319475, Length=289, Percent_Identity=33.9100346020761, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=25.9911894273128, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=29.8657718120805, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281365769, Length=270, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24660002, Length=270, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36162; Mature: 36031

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQPESTMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
VLAARAVSYEGEAHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
DFLYTRSFQMMTELDSMKVLRVLADTTNVLAEGEVLQLMNCNDPDTTEESYMRVIYCKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHEEEHEEHHH
KLFEAATLLAGVLAGATPEQETALGNYGKYLGTAFQLTDDLLDYTADSDELGKNIGDDLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHH
EGKPTLPLIYAIAHGTDEEKHLIRQAIEQGDGTQAIDKIVAALHHCGALNYTQQKAIEES
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EKAIEALSILPDSDYKQALVALAKISVDRNH
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TPQPESTMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
VLAARAVSYEGEAHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
DFLYTRSFQMMTELDSMKVLRVLADTTNVLAEGEVLQLMNCNDPDTTEESYMRVIYCKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHEEEHEEHHH
KLFEAATLLAGVLAGATPEQETALGNYGKYLGTAFQLTDDLLDYTADSDELGKNIGDDLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHH
EGKPTLPLIYAIAHGTDEEKHLIRQAIEQGDGTQAIDKIVAALHHCGALNYTQQKAIEES
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EKAIEALSILPDSDYKQALVALAKISVDRNH
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]