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The map label for this gene is yusP

Identifier: 16080340

GI number: 16080340

Start: 3374956

End: 3376581

Strand: Direct

Name: yusP

Synonym: BSU32880

Alternate gene names: 16080340

Gene position: 3374956-3376581 (Clockwise)

Preceding gene: 16080339

Following gene: 16080344

Centisome position: 80.06

GC content: 44.77

Gene sequence:

>1626_bases
ATGAAAATGAGTAAAGAAGGCAAACAATCAAAACGAACGTTATTAATAACAGGCTTAATTATTGCCATGTTTTTTTCAGC
CCTTGACGGTACCATTGTCGGAACGGCCATGCCGAAAATTGTCGGTGATCTGGGCGGTTTGAGCATGATGACCTGGCTGA
CAACTGCATATTTGTTAACATCAACAACCATTGTTCCAATTGCCGGTAAACTGGCTGATTTATTGGGACGCCGCATTGTC
TATGTATCAGGTTTGATTATTTTTATGGCCGCTTCTGCTTTGTGCGGAATGGCCAACAACATGACGGAATTGATTATCTT
CCGTGGACTGCAGGGCATCGGCGGAGGCATCATGATGCCGATGGCGATGATTGTCATCGGTGACTTGTTCACAGGAAAAC
AGCGTGCTAAATTCCAAGGTGTCTTTGGCGCAATTTACGGGCTGGCTTCAGTGATTGGGCCGCAAATCGGCGGCTGGATT
GTAGACTCGTTAAACTGGAAATGGGTTTTCTATATTAACCTGCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGG
ATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCC
TCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTG
ATCGGCATTGTCAGCTTTATCATCGTAGAATCAAAAGCAAAAGAGCCAATCCTGCCAATGTATTTATTTAAAAACAGAAC
ATTTACATTTCTAAACTTGATCGGCTTCTTTATGAGTATCGGTATGTTTGGCGCCATTACGTTTGTTCCTTTCTTTATGC
AGGGAATTGTCGGTGTAAGTGCGTCTGAATCGGGTACAATTATGACACCGATGATGATTTCTATGATTATCACAAGTATC
ATCGGCGGACAGCTTGTTTATAAAATTGGGATTAAACCGCAAATCATTACAGGCATGCTGGTTATGGCCGGCGGATTCCT
GCTGTTAACGACATTGGATCTCGACACGAGCAAGCTTGTCGCTACATCTTTTATGGCGATTATTGGTCTGGGAATGGGTC
TTGTTATGCCGATTCTGACATTGGCTTTACAGGAAAGCTTTTCGAAGGAGGAGCTTGGAGTTGTCACTTCATCCAGCCAA
TTCTTCCGTTCTATCGGCGGAACATTCGGCATTACCATGCTGGGAGCCGTAATGAATGCGAGATCAGGAAATCTATTAAC
AGATAAATTGGTTCCATATTTAGACTCTCTTCCGGCACAGGCAAGCTCGTTTGCCGCCCAGCTAAAGGGTATGATTGATA
CAAATCCGCAAGGGCTGCTGCAATCACTGTTCAGTCCGGACGCGGTCAAACAAATCCCTGCGGCTTTCTCATCCCATATT
GTGCCGATTTTAAAAACATCGTTGATGGATTCGCTCCACAGCGTGTTTTATACGGGCCTGATCTTTATCGCAGTCGGAGC
CGTATTCACCTTGTTCTTAAAACCTATCAAGCTATCAAATAAGAAAGCGGAAGACCAACAAGAAAAAGAGAAGGCGGCAA
AAGCCGTTGAATCACCTTCCCATTAA

Upstream 100 bases:

>100_bases
TTTTGACGGAGGAGGAAATGCTGCAGGCGGCGCATATTACAGCAAAACTCGCACAAGCAGCAGAAACGGATGAAAAGCAA
AACATGAAAAGAGGAAACGG

Downstream 100 bases:

>100_bases
TAGAGCACCCCGCGGGTATCAATCCGCGGGGTTTTTTATTCAATCATCTGGGCTCTTCCATTTCCGAAAGACCAATCCTC
ATCCTGATTTCCGGCCAGCA

Product: multidrug-efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKMSKEGKQSKRTLLITGLIIAMFFSALDGTIVGTAMPKIVGDLGGLSMMTWLTTAYLLTSTTIVPIAGKLADLLGRRIV
YVSGLIIFMAASALCGMANNMTELIIFRGLQGIGGGIMMPMAMIVIGDLFTGKQRAKFQGVFGAIYGLASVIGPQIGGWI
VDSLNWKWVFYINLPVGIIAVIFIARGLQGRQQTGPINFDIAGIFTMIVGVVSLLLALSFGGKDYAWDSWQILGLFALAL
IGIVSFIIVESKAKEPILPMYLFKNRTFTFLNLIGFFMSIGMFGAITFVPFFMQGIVGVSASESGTIMTPMMISMIITSI
IGGQLVYKIGIKPQIITGMLVMAGGFLLLTTLDLDTSKLVATSFMAIIGLGMGLVMPILTLALQESFSKEELGVVTSSSQ
FFRSIGGTFGITMLGAVMNARSGNLLTDKLVPYLDSLPAQASSFAAQLKGMIDTNPQGLLQSLFSPDAVKQIPAAFSSHI
VPILKTSLMDSLHSVFYTGLIFIAVGAVFTLFLKPIKLSNKKAEDQQEKEKAAKAVESPSH

Sequences:

>Translated_541_residues
MKMSKEGKQSKRTLLITGLIIAMFFSALDGTIVGTAMPKIVGDLGGLSMMTWLTTAYLLTSTTIVPIAGKLADLLGRRIV
YVSGLIIFMAASALCGMANNMTELIIFRGLQGIGGGIMMPMAMIVIGDLFTGKQRAKFQGVFGAIYGLASVIGPQIGGWI
VDSLNWKWVFYINLPVGIIAVIFIARGLQGRQQTGPINFDIAGIFTMIVGVVSLLLALSFGGKDYAWDSWQILGLFALAL
IGIVSFIIVESKAKEPILPMYLFKNRTFTFLNLIGFFMSIGMFGAITFVPFFMQGIVGVSASESGTIMTPMMISMIITSI
IGGQLVYKIGIKPQIITGMLVMAGGFLLLTTLDLDTSKLVATSFMAIIGLGMGLVMPILTLALQESFSKEELGVVTSSSQ
FFRSIGGTFGITMLGAVMNARSGNLLTDKLVPYLDSLPAQASSFAAQLKGMIDTNPQGLLQSLFSPDAVKQIPAAFSSHI
VPILKTSLMDSLHSVFYTGLIFIAVGAVFTLFLKPIKLSNKKAEDQQEKEKAAKAVESPSH
>Mature_541_residues
MKMSKEGKQSKRTLLITGLIIAMFFSALDGTIVGTAMPKIVGDLGGLSMMTWLTTAYLLTSTTIVPIAGKLADLLGRRIV
YVSGLIIFMAASALCGMANNMTELIIFRGLQGIGGGIMMPMAMIVIGDLFTGKQRAKFQGVFGAIYGLASVIGPQIGGWI
VDSLNWKWVFYINLPVGIIAVIFIARGLQGRQQTGPINFDIAGIFTMIVGVVSLLLALSFGGKDYAWDSWQILGLFALAL
IGIVSFIIVESKAKEPILPMYLFKNRTFTFLNLIGFFMSIGMFGAITFVPFFMQGIVGVSASESGTIMTPMMISMIITSI
IGGQLVYKIGIKPQIITGMLVMAGGFLLLTTLDLDTSKLVATSFMAIIGLGMGLVMPILTLALQESFSKEELGVVTSSSQ
FFRSIGGTFGITMLGAVMNARSGNLLTDKLVPYLDSLPAQASSFAAQLKGMIDTNPQGLLQSLFSPDAVKQIPAAFSSHI
VPILKTSLMDSLHSVFYTGLIFIAVGAVFTLFLKPIKLSNKKAEDQQEKEKAAKAVESPSH

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1789042, Length=463, Percent_Identity=26.7818574514039, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1788710, Length=428, Percent_Identity=26.8691588785047, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1790195, Length=326, Percent_Identity=30.9815950920245, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=25, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788392, Length=346, Percent_Identity=28.9017341040462, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=27.1604938271605, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=576, Percent_Identity=25.3472222222222, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323735, Length=337, Percent_Identity=27.0029673590504, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319770, Length=406, Percent_Identity=26.8472906403941, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319783, Length=489, Percent_Identity=24.1308793456033, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321663, Length=365, Percent_Identity=25.7534246575342, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=422, Percent_Identity=23.9336492890995, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=377, Percent_Identity=25.1989389920424, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=365, Percent_Identity=24.9315068493151, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUSP_BACSU (O32182)

Other databases:

- EMBL:   AL009126
- PIR:   A70022
- RefSeq:   NP_391167.1
- ProteinModelPortal:   O32182
- EnsemblBacteria:   EBBACT00000003692
- GeneID:   937088
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32880
- NMPDR:   fig|224308.1.peg.3293
- GenoList:   BSU32880
- GeneTree:   EBGT00070000031956
- HOGENOM:   HBG679341
- OMA:   MPMAMIV
- PhylomeDB:   O32182
- ProtClustDB:   CLSK887848
- BioCyc:   BSUB:BSU32880-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 57947; Mature: 57947

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1235c1c4)-; HASH(0x123365c0)-; HASH(0x122f6e80)-; HASH(0x1233f214)-; HASH(0x124b30b8)-; HASH(0x11ee5014)-; HASH(0xbb93cd4)-; HASH(0x123b8a84)-; HASH(0xfdd5014)-; HASH(0x108095e8)-; HASH(0xf9a2874)-; HASH(0x124cf7dc)-; HASH(0x11bce788)-; HASH(0x11fb6408)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSKEGKQSKRTLLITGLIIAMFFSALDGTIVGTAMPKIVGDLGGLSMMTWLTTAYLLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
STTIVPIAGKLADLLGRRIVYVSGLIIFMAASALCGMANNMTELIIFRGLQGIGGGIMMP
HCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHH
MAMIVIGDLFTGKQRAKFQGVFGAIYGLASVIGPQIGGWIVDSLNWKWVFYINLPVGIIA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCHHHHH
VIFIARGLQGRQQTGPINFDIAGIFTMIVGVVSLLLALSFGGKDYAWDSWQILGLFALAL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IGIVSFIIVESKAKEPILPMYLFKNRTFTFLNLIGFFMSIGMFGAITFVPFFMQGIVGVS
HHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASESGTIMTPMMISMIITSIIGGQLVYKIGIKPQIITGMLVMAGGFLLLTTLDLDTSKLV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHH
ATSFMAIIGLGMGLVMPILTLALQESFSKEELGVVTSSSQFFRSIGGTFGITMLGAVMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEECCHHHHHHCCCHHHHHHHHHHHHC
RSGNLLTDKLVPYLDSLPAQASSFAAQLKGMIDTNPQGLLQSLFSPDAVKQIPAAFSSHI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VPILKTSLMDSLHSVFYTGLIFIAVGAVFTLFLKPIKLSNKKAEDQQEKEKAAKAVESPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
H
H
>Mature Secondary Structure
MKMSKEGKQSKRTLLITGLIIAMFFSALDGTIVGTAMPKIVGDLGGLSMMTWLTTAYLLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
STTIVPIAGKLADLLGRRIVYVSGLIIFMAASALCGMANNMTELIIFRGLQGIGGGIMMP
HCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHH
MAMIVIGDLFTGKQRAKFQGVFGAIYGLASVIGPQIGGWIVDSLNWKWVFYINLPVGIIA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCHHHHH
VIFIARGLQGRQQTGPINFDIAGIFTMIVGVVSLLLALSFGGKDYAWDSWQILGLFALAL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IGIVSFIIVESKAKEPILPMYLFKNRTFTFLNLIGFFMSIGMFGAITFVPFFMQGIVGVS
HHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASESGTIMTPMMISMIITSIIGGQLVYKIGIKPQIITGMLVMAGGFLLLTTLDLDTSKLV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHH
ATSFMAIIGLGMGLVMPILTLALQESFSKEELGVVTSSSQFFRSIGGTFGITMLGAVMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEECCHHHHHHCCCHHHHHHHHHHHHC
RSGNLLTDKLVPYLDSLPAQASSFAAQLKGMIDTNPQGLLQSLFSPDAVKQIPAAFSSHI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
VPILKTSLMDSLHSVFYTGLIFIAVGAVFTLFLKPIKLSNKKAEDQQEKEKAAKAVESPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
H
H

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377