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The map label for this gene is pucK

Identifier: 16080297

GI number: 16080297

Start: 3331857

End: 3333149

Strand: Direct

Name: pucK

Synonym: BSU32440

Alternate gene names: 16080297

Gene position: 3331857-3333149 (Clockwise)

Preceding gene: 16080296

Following gene: 16080298

Centisome position: 79.04

GC content: 51.51

Gene sequence:

>1293_bases
ATGAAGGAACAGCACAATGCCTTACAGCTAATGATGCTGGGGCTTCAGCACATGCTTGCCATGTATGCGGGAGCCATTCT
CGTTCCGTTGATCGTCGGAGCAGCGATCGGGCTGAATGCGGGGCAGCTGACGTATTTAATCGCGATTGATTTGTTTATGT
GCGGGGCGGCAACTCTTTTGCAGCTCTGGAGAAACCGATATTTCGGCATCGGCCTTCCCGTTGTGCTCGGATGCACGTTT
ACCGCAGTCGGCCCGATGATTTCCATCGGAAGCACTTACGGTGTCCCAGCGATATATGGTGCCATTATCGCCGCAGGACT
GATTGTCGTCTTAGCCGCGGGCTTCTTCGGGAAGCTTGTCCGTTTTTTTCCGCCTGTCGTAACCGGATCAGTCGTCATGA
TCATCGGCATCAGCCTGATTCCAACGGCAATGAATAACCTGGCCGGAGGTGAAGGAAGCAAAGAATTCGGCTCACTCGAC
AACGTGCTCCTCGGGTTTGGCGTCACAGCTTTTATCTTATTGCTGTTTTATTTCTTTAAAGGCTTTATCCGCTCCATAGC
GATTTTACTCGGTCTTATCGCAGGAACAGCGGCTGCATATTTCATGGGAAAGGTCGATTTTTCTGAGGTTTTGGAGGCGT
CTTGGCTTCATGTTCCGTCTCTGTTTTATTTCGGACCGCCAACCTTTGAGCTGCCGGCTGTTGTCACGATGCTTCTCGTT
GCAATTGTCAGCCTCGTTGAATCGACAGGCGTTTATTTTGCCCTCGCTGATATCACAAACCGCAGGCTGTCGGAGAAGGA
TCTGGAAAAAGGCTACCGGGCAGAAGGCCTTGCGATTTTGCTGGGCGGCTTATTTAACGCGTTTCCCTATACAGCTTTCT
CGCAGAATGTCGGCATCGTTCAGCTCTCAAAGATGAAAAGTGTCAACGTCATCGCCATCACAGGAATCATACTGGTCGCG
ATCGGGCTGGTTCCAAAAGCAGCGGCCCTGACAACAGTGATCCCCACCCCAGTTCTCGGCGGCGCAATGATCGTCATGTT
CGGCATGGTCATTTCTTACGGGATCAAAATGCTATCCAGCGTTGACCTTGATTCGCAAGGCAACCTGCTGATTATCGCCT
CTTCCGTCAGCTTGGGGCTTGGCGCTACAACAGTTCCCGCGTTGTTTTCATCTCTTTCGGGCGCGGCCTCTGTCTTGGCA
GGAAGCGGAATTGTGATCGGCAGTTTGACGGCCATTGCACTGCACGCTTTTTTTCAAACCAAACAGCCGAATAGCGCAGA
CATAAAAACGTAA

Upstream 100 bases:

>100_bases
TATTTTTCAGCTTGCGCGACAAAAAAGAATTAACAGCACAGCAAACAGAACTTCCGGTATTAGAACATACACTCGCATTA
GAAAAAGAGGTGTAAAAAAG

Downstream 100 bases:

>100_bases
AGGAAGATGCCAATGTTCACAATGGATGACCTGAACCAAATGGACACACAAACACTGACAGACACACTTGGCTCTATTTT
TGAACACTCTTCATGGATTG

Product: uric acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKEQHNALQLMMLGLQHMLAMYAGAILVPLIVGAAIGLNAGQLTYLIAIDLFMCGAATLLQLWRNRYFGIGLPVVLGCTF
TAVGPMISIGSTYGVPAIYGAIIAAGLIVVLAAGFFGKLVRFFPPVVTGSVVMIIGISLIPTAMNNLAGGEGSKEFGSLD
NVLLGFGVTAFILLLFYFFKGFIRSIAILLGLIAGTAAAYFMGKVDFSEVLEASWLHVPSLFYFGPPTFELPAVVTMLLV
AIVSLVESTGVYFALADITNRRLSEKDLEKGYRAEGLAILLGGLFNAFPYTAFSQNVGIVQLSKMKSVNVIAITGIILVA
IGLVPKAAALTTVIPTPVLGGAMIVMFGMVISYGIKMLSSVDLDSQGNLLIIASSVSLGLGATTVPALFSSLSGAASVLA
GSGIVIGSLTAIALHAFFQTKQPNSADIKT

Sequences:

>Translated_430_residues
MKEQHNALQLMMLGLQHMLAMYAGAILVPLIVGAAIGLNAGQLTYLIAIDLFMCGAATLLQLWRNRYFGIGLPVVLGCTF
TAVGPMISIGSTYGVPAIYGAIIAAGLIVVLAAGFFGKLVRFFPPVVTGSVVMIIGISLIPTAMNNLAGGEGSKEFGSLD
NVLLGFGVTAFILLLFYFFKGFIRSIAILLGLIAGTAAAYFMGKVDFSEVLEASWLHVPSLFYFGPPTFELPAVVTMLLV
AIVSLVESTGVYFALADITNRRLSEKDLEKGYRAEGLAILLGGLFNAFPYTAFSQNVGIVQLSKMKSVNVIAITGIILVA
IGLVPKAAALTTVIPTPVLGGAMIVMFGMVISYGIKMLSSVDLDSQGNLLIIASSVSLGLGATTVPALFSSLSGAASVLA
GSGIVIGSLTAIALHAFFQTKQPNSADIKT
>Mature_430_residues
MKEQHNALQLMMLGLQHMLAMYAGAILVPLIVGAAIGLNAGQLTYLIAIDLFMCGAATLLQLWRNRYFGIGLPVVLGCTF
TAVGPMISIGSTYGVPAIYGAIIAAGLIVVLAAGFFGKLVRFFPPVVTGSVVMIIGISLIPTAMNNLAGGEGSKEFGSLD
NVLLGFGVTAFILLLFYFFKGFIRSIAILLGLIAGTAAAYFMGKVDFSEVLEASWLHVPSLFYFGPPTFELPAVVTMLLV
AIVSLVESTGVYFALADITNRRLSEKDLEKGYRAEGLAILLGGLFNAFPYTAFSQNVGIVQLSKMKSVNVIAITGIILVA
IGLVPKAAALTTVIPTPVLGGAMIVMFGMVISYGIKMLSSVDLDSQGNLLIIASSVSLGLGATTVPALFSSLSGAASVLA
GSGIVIGSLTAIALHAFFQTKQPNSADIKT

Specific function: Uptake of uric acid

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Homo sapiens, GI44680148, Length=435, Percent_Identity=27.1264367816092, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40316845, Length=435, Percent_Identity=27.1264367816092, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI44680145, Length=448, Percent_Identity=25.4464285714286, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI44680143, Length=452, Percent_Identity=25.2212389380531, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI87082181, Length=425, Percent_Identity=41.8823529411765, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790087, Length=439, Percent_Identity=30.5239179954442, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082178, Length=431, Percent_Identity=30.1624129930394, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788843, Length=406, Percent_Identity=27.8325123152709, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081818, Length=401, Percent_Identity=29.1770573566085, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17558856, Length=196, Percent_Identity=31.1224489795918, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17542262, Length=419, Percent_Identity=23.8663484486874, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17541904, Length=415, Percent_Identity=23.3734939759036, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17542260, Length=185, Percent_Identity=29.1891891891892, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993493, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356175, Length=422, Percent_Identity=25.8293838862559, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCK_BACSU (O32140)

Other databases:

- EMBL:   AL009126
- PIR:   F70016
- RefSeq:   NP_391124.1
- ProteinModelPortal:   O32140
- EnsemblBacteria:   EBBACT00000002131
- GeneID:   937191
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32440
- NMPDR:   fig|224308.1.peg.3250
- GenoList:   BSU32440
- GeneTree:   EBGT00050000001001
- HOGENOM:   HBG470187
- OMA:   ARLPLIQ
- PhylomeDB:   O32140
- ProtClustDB:   CLSK2752500
- BioCyc:   BSUB:BSU32440-MONOMER
- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043
- PANTHER:   PTHR11119:SF3
- PANTHER:   PTHR11119
- TIGRFAMs:   TIGR00801
- TIGRFAMs:   TIGR03173

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 44862; Mature: 44862

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb754d04)-; HASH(0x12322378)-; HASH(0xf9eecc4)-; HASH(0xeb205cc)-; HASH(0xfec7114)-; HASH(0xc875610)-; HASH(0x118670fc)-; HASH(0x1178ee30)-; HASH(0x104f84c8)-; HASH(0xfe2acd4)-; HASH(0x11ff7598)-; HASH(0x121688fc)-; HASH(0x11f34d14)-; HASH(0x116b2f94)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCH
>Mature Secondary Structure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CCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136