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The map label for this gene is msmR

Identifier: 16080078

GI number: 16080078

Start: 3096782

End: 3097816

Strand: Direct

Name: msmR

Synonym: BSU30260

Alternate gene names: 16080078

Gene position: 3096782-3097816 (Clockwise)

Preceding gene: 16080067

Following gene: 16080079

Centisome position: 73.46

GC content: 48.99

Gene sequence:

>1035_bases
ATGGTTCGTATTAAAGATATCGCCTTGAAAGCTAAAGTTTCCAGCGCAACTGTGTCCAGAATTTTAAATGAAGATGAGTC
GCTTTCTGTTGCGGGCGAAACGAGACAAAGAGTCATCAACATCGCTGAAGAGCTTGGTTATCAAACCGTTGCCAAACGCC
GAAAATCCCGCGGGCAAAAACAGCGGGCTCAGCCGCTGATCGGTGTGCTGAGCTGTCTGTCCCCTGATCAGGAAAGGCAG
GACCCTTATTTTTCTTCCATTCGGAAAGGGATTGAAAAGGAATGCTTTGAACAGGAAATTTTCATTACAAATTCGATTCA
TCTCGGCTCCTTTCAGGAACATATCTTTCGGGAATTGGATGGTGTCATTGTCATCGGCCGTGTTCATGATGAAGCGGTTA
AGCATATCAGCGGGAGGCTGGAGCATGCCGTATTTATCAATCATTCACCAGATCCGCAAGCATACGATTCGATTGGCATC
GATTTTGAATCGGCTTCACGCCAGGCGATTGATCACCTTTTCGACTTAGGCTACAAACGGTTAGGCTACATTGGCGGACA
AGAAAAAGAGCATACGCTGAAGGACGGCCAAAGCATTCGCAGAACGATTGAAGATAAACGCCTGACCGCTTTTTTGGAGT
CAGCCGCCCCCCAGCCTGAGCATGTGCTGATCGGAGAATACAGCATGCGTGAGGGCTATCGCCTGATGAAGAAAGCAATC
GATCAAGGCCATCTGCCGGAAGCATTCTTTATTGCCAGCGATTCTATGGCGATCGGCGCATTAAAAGCGCTGCAGGAAGC
CGGACTGCAAGTGCCGCGGGATACCGCAATCGTCAGCTTTAACGGCATTGAGGAAGCTGAATTTGCCAGCACGCCTTTAA
CGACGGTGAAGGTATACACAGAGGAAATGGGCCGGACAGGCGTAAAACTGCTGCTTGACCGTCTCAATGGCCGAACGCTT
CCTCAACATGTCACCCTGCCTACAACATTAATCGTAAGACAAAGCTGCGGATGTACAGCAAAGGAGGTGACATAA

Upstream 100 bases:

>100_bases
TTCGAAAACAATTATTGTAACCGCTTACTTTTATATGATAATATCAATTTATCAAAAACAGATGAGTTAATATTTTACTA
AATAGATGAGAGGGATACCC

Downstream 100 bases:

>100_bases
GCAAAGATTCATCACGATGATAAGGAGGAAAAGATGAAACACACTTTTGTTTTATTTCTCTCTCTTATTCTGCTTGTTCT
GCCCGGGTGTTCAGCAGAGA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQKQRAQPLIGVLSCLSPDQERQ
DPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELDGVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGI
DFESASRQAIDHLFDLGYKRLGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAI
DQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYTEEMGRTGVKLLLDRLNGRTL
PQHVTLPTTLIVRQSCGCTAKEVT

Sequences:

>Translated_344_residues
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQKQRAQPLIGVLSCLSPDQERQ
DPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELDGVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGI
DFESASRQAIDHLFDLGYKRLGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAI
DQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYTEEMGRTGVKLLLDRLNGRTL
PQHVTLPTTLIVRQSCGCTAKEVT
>Mature_344_residues
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQKQRAQPLIGVLSCLSPDQERQ
DPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELDGVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGI
DFESASRQAIDHLFDLGYKRLGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAI
DQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYTEEMGRTGVKLLLDRLNGRTL
PQHVTLPTTLIVRQSCGCTAKEVT

Specific function: Repressor For Beta Galactosidase Alpha And Beta Subunits (Ebga And Ebgc). Binds Lactose As An Inducer. [C]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=34.1176470588235, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=31.6417910447761, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787948, Length=349, Percent_Identity=28.9398280802292, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=26.4264264264264, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=26.878612716763, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786540, Length=352, Percent_Identity=25.2840909090909, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790689, Length=335, Percent_Identity=27.7611940298507, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994940, Length=338, Percent_Identity=26.3313609467456, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787580, Length=362, Percent_Identity=26.2430939226519, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789202, Length=343, Percent_Identity=25.3644314868805, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789068, Length=342, Percent_Identity=24.2690058479532, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSMR_BACSU (O34829)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   A69661
- RefSeq:   NP_390904.1
- HSSP:   P46828
- ProteinModelPortal:   O34829
- SMR:   O34829
- EnsemblBacteria:   EBBACT00000002512
- GeneID:   937257
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30260
- NMPDR:   fig|224308.1.peg.3029
- GenoList:   BSU30260
- GeneTree:   EBGT00070000032245
- HOGENOM:   HBG753640
- PhylomeDB:   O34829
- ProtClustDB:   CLSK873257
- BioCyc:   BSUB:BSU30260-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 38399; Mature: 38399

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQK
CCEEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCHH
QRAQPLIGVLSCLSPDQERQDPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELD
HHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHHCC
GVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGIDFESASRQAIDHLFDLGYKR
CEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
DQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYT
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCHHEEHHH
EEMGRTGVKLLLDRLNGRTLPQHVTLPTTLIVRQSCGCTAKEVT
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQK
CCEEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCHH
QRAQPLIGVLSCLSPDQERQDPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELD
HHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHHCC
GVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGIDFESASRQAIDHLFDLGYKR
CEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
DQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYT
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCHHEEHHH
EEMGRTGVKLLLDRLNGRTLPQHVTLPTTLIVRQSCGCTAKEVT
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377