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The map label for this gene is purA [H]

Identifier: 159901106

GI number: 159901106

Start: 5875209

End: 5876495

Strand: Direct

Name: purA [H]

Synonym: Haur_4594

Alternate gene names: 159901106

Gene position: 5875209-5876495 (Clockwise)

Preceding gene: 159901105

Following gene: 159901115

Centisome position: 92.57

GC content: 52.06

Gene sequence:

>1287_bases
ATGCCAGTCGTTGCAGTTCTTGGCGCTCAATGGGGCGACGAGGGCAAAGGTCGTGTTGTCGATTTATTGGCAACCAAAGC
TAAAATGGTTATTCGCGCTGCTGGTGGCTCTAATGCCGGCCATACGGTGATTAACCATCTTGGCACGTTTAAATTGCACC
TCACGCCTGCCGGAATTTTCGATGCGAATGTCGTCAATATTATCGGCGCAGGCACCGTGATCGATCCTGCGGTGTTGATG
AAGGAGTTGCAGGCCTTACGTGAGGCCAATGTTTCGCTTGATCAATTATATATCAGCGATCGGGCACATGTGGTTATGCC
CTATCACGTTTCGCTCGATGCGCTCGAAGAAAAAGATCGCGGCGCGAACGAAATTGGCACAACTAAGCGTGGTATTGGCC
CAGCCTACGTCGATAAAGCGTCGCGCATGGGCATTCGTATGGGCGATTTGCTGCACGAAGAAACCTTGCTTAGTCGCCTA
ACCAGCGTGCTCGAATACAAGGGCCGCGTGTTGAATAAGATGTATGGCGCTCAGCCAAGCGCTTCATTGCATGATATGTA
TCTGCAATATTTGGAGTTTGGTCGCCAACTCGAAGCCCACATCGTGCCCATCCACACCATGGTTCAAGATGCCTTGCGCC
GCGATATTCCAATTTTGATCGAGGGCAATCAAGGAGCATTATTGGATGTGGATTATGGCACATTCCCGTATGTGACTTCA
ACTGCAACTGGCTCTGCGGGCGCGTGCCAAGGCGCGGGAATTCCGCCGATGCGCTTAAATGGTGTAGTCGGAATTTACAA
AGCCTACACAACCCGCGTTGGCGGTGGGCCATTCCCAACCGAATTAACCGATGAACTTGGTGAGTATATTCGCCAAGCCG
GCCATGAGTTTGGCACAACCACTGGTCGCCCACGACGGGTTGGCTGGTTCGATGCGGTGGCAGCTCGCTATGCTGCCGAA
ATTAATGGCATTAGCTCGATCGCCCTTACCAAACTCGATGTGCTCGACGATGTTGAGACGCTCAAAATTTGTACGGGCTA
TCGCTGGAACGACACCGAGCTTGATTCATTCCCATCGTCAATTTCGGTGCTCAGCCAAGTTGAGCCAATTTACGAAGAAT
ATCCTGGTTGGAAAACCAACACCAGCCATGCCCGCACCTTGGATGAGTTGCCCGAAGCGGCGCAACGCTATGTTCGCCGT
CTCAGTCAATTGGTTGGCGTGCGCTTGGGCATGATTTCGGTCGGCCCTTCGCGTGAGCAAATGGTGAGTTTGCAAGAAAT
CTTCTAA

Upstream 100 bases:

>100_bases
CCAAATATGGCTTTTTGACGGTTTGGGCGTTATTCCGGCTGCGTTTGCTCGGTCGCCCATAACCACGGTATTTGCTCCTC
TTACAGAAAAGGTGATACAT

Downstream 100 bases:

>100_bases
ATTTTCATCCTTGCATATGGAATAAAAGCAAAGCCTCGGCTTGCTGATCTGGTTTGGCGTTTGTCTAACTAAATCACCAA
GCCGAGGCTTTCGCGCCAAT

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPVVAVLGAQWGDEGKGRVVDLLATKAKMVIRAAGGSNAGHTVINHLGTFKLHLTPAGIFDANVVNIIGAGTVIDPAVLM
KELQALREANVSLDQLYISDRAHVVMPYHVSLDALEEKDRGANEIGTTKRGIGPAYVDKASRMGIRMGDLLHEETLLSRL
TSVLEYKGRVLNKMYGAQPSASLHDMYLQYLEFGRQLEAHIVPIHTMVQDALRRDIPILIEGNQGALLDVDYGTFPYVTS
TATGSAGACQGAGIPPMRLNGVVGIYKAYTTRVGGGPFPTELTDELGEYIRQAGHEFGTTTGRPRRVGWFDAVAARYAAE
INGISSIALTKLDVLDDVETLKICTGYRWNDTELDSFPSSISVLSQVEPIYEEYPGWKTNTSHARTLDELPEAAQRYVRR
LSQLVGVRLGMISVGPSREQMVSLQEIF

Sequences:

>Translated_428_residues
MPVVAVLGAQWGDEGKGRVVDLLATKAKMVIRAAGGSNAGHTVINHLGTFKLHLTPAGIFDANVVNIIGAGTVIDPAVLM
KELQALREANVSLDQLYISDRAHVVMPYHVSLDALEEKDRGANEIGTTKRGIGPAYVDKASRMGIRMGDLLHEETLLSRL
TSVLEYKGRVLNKMYGAQPSASLHDMYLQYLEFGRQLEAHIVPIHTMVQDALRRDIPILIEGNQGALLDVDYGTFPYVTS
TATGSAGACQGAGIPPMRLNGVVGIYKAYTTRVGGGPFPTELTDELGEYIRQAGHEFGTTTGRPRRVGWFDAVAARYAAE
INGISSIALTKLDVLDDVETLKICTGYRWNDTELDSFPSSISVLSQVEPIYEEYPGWKTNTSHARTLDELPEAAQRYVRR
LSQLVGVRLGMISVGPSREQMVSLQEIF
>Mature_427_residues
PVVAVLGAQWGDEGKGRVVDLLATKAKMVIRAAGGSNAGHTVINHLGTFKLHLTPAGIFDANVVNIIGAGTVIDPAVLMK
ELQALREANVSLDQLYISDRAHVVMPYHVSLDALEEKDRGANEIGTTKRGIGPAYVDKASRMGIRMGDLLHEETLLSRLT
SVLEYKGRVLNKMYGAQPSASLHDMYLQYLEFGRQLEAHIVPIHTMVQDALRRDIPILIEGNQGALLDVDYGTFPYVTST
ATGSAGACQGAGIPPMRLNGVVGIYKAYTTRVGGGPFPTELTDELGEYIRQAGHEFGTTTGRPRRVGWFDAVAARYAAEI
NGISSIALTKLDVLDDVETLKICTGYRWNDTELDSFPSSISVLSQVEPIYEEYPGWKTNTSHARTLDELPEAAQRYVRRL
SQLVGVRLGMISVGPSREQMVSLQEIF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=440, Percent_Identity=42.2727272727273, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI34577063, Length=438, Percent_Identity=41.7808219178082, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI40316944, Length=408, Percent_Identity=40.1960784313725, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=44.9648711943794, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=39.1705069124424, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=39.1705069124424, Blast_Score=303, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=38.8888888888889, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI21356033, Length=430, Percent_Identity=40.4651162790698, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46668; Mature: 46537

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVAVLGAQWGDEGKGRVVDLLATKAKMVIRAAGGSNAGHTVINHLGTFKLHLTPAGIF
CCEEEEECCCCCCCCCCCEEHHHHHHHHEEEEECCCCCCCHHHHHHCCEEEEEEECCCCC
DANVVNIIGAGTVIDPAVLMKELQALREANVSLDQLYISDRAHVVMPYHVSLDALEEKDR
CCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEEEEEEECCHHHHHHHCC
GANEIGTTKRGIGPAYVDKASRMGIRMGDLLHEETLLSRLTSVLEYKGRVLNKMYGAQPS
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ASLHDMYLQYLEFGRQLEAHIVPIHTMVQDALRRDIPILIEGNQGALLDVDYGTFPYVTS
CHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEE
TATGSAGACQGAGIPPMRLNGVVGIYKAYTTRVGGGPFPTELTDELGEYIRQAGHEFGTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCCC
TGRPRRVGWFDAVAARYAAEINGISSIALTKLDVLDDVETLKICTGYRWNDTELDSFPSS
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHCCHH
ISVLSQVEPIYEEYPGWKTNTSHARTLDELPEAAQRYVRRLSQLVGVRLGMISVGPSREQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHH
MVSLQEIF
HHHHHHCC
>Mature Secondary Structure 
PVVAVLGAQWGDEGKGRVVDLLATKAKMVIRAAGGSNAGHTVINHLGTFKLHLTPAGIF
CEEEEECCCCCCCCCCCEEHHHHHHHHEEEEECCCCCCCHHHHHHCCEEEEEEECCCCC
DANVVNIIGAGTVIDPAVLMKELQALREANVSLDQLYISDRAHVVMPYHVSLDALEEKDR
CCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEEEEEEECCHHHHHHHCC
GANEIGTTKRGIGPAYVDKASRMGIRMGDLLHEETLLSRLTSVLEYKGRVLNKMYGAQPS
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ASLHDMYLQYLEFGRQLEAHIVPIHTMVQDALRRDIPILIEGNQGALLDVDYGTFPYVTS
CHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEE
TATGSAGACQGAGIPPMRLNGVVGIYKAYTTRVGGGPFPTELTDELGEYIRQAGHEFGTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCCC
TGRPRRVGWFDAVAARYAAEINGISSIALTKLDVLDDVETLKICTGYRWNDTELDSFPSS
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHCCHH
ISVLSQVEPIYEEYPGWKTNTSHARTLDELPEAAQRYVRRLSQLVGVRLGMISVGPSREQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHH
MVSLQEIF
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA