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The map label for this gene is leuS

Identifier: 159900932

GI number: 159900932

Start: 5654065

End: 5656509

Strand: Direct

Name: leuS

Synonym: Haur_4419

Alternate gene names: 159900932

Gene position: 5654065-5656509 (Clockwise)

Preceding gene: 159900929

Following gene: 159900933

Centisome position: 89.09

GC content: 50.88

Gene sequence:

>2445_bases
ATGGCCACTCGCTATGATAGTAGCATCACCGAGCCAAAATGGCGCGAACGTTGGGAACGCGACGGCATTTATCGCTTCGA
GCCAGACAGCGATAAGCCTAAACACTATGCCGTCACTATGTTGCCCTATCCATCGGGCAATTTGCACATTGGCCATTGGT
ATGCCATGTCGCCCAGCGACGTGCATGCCCGCTACATGCGCATGCGTGGCTACAACGTCTTTTTCCCAATGGGCTTCGAT
GCCTTTGGCCTGCCCGCTGAAAACGCCGCAATCAAAAACAACTTAGATCCGCGCAAATGGACCTATTCCAACATTGAATA
TATGCGCGGCCAATTGCAATCGATGGGCATGATGGTCGATTGGGATCAGCAAATTGTTAGCGCCGACCCTGAATATTATC
GCTGGAATCAGTGGTTTTTTATTCAGTTTTTCAAGCGTGGCTTAGCCTACAAAAAATTCTCAGCCGTCGATTGGTGTCCC
AAGTGTAATACAACCTTGGCCCGCGAACAGGTTGTGGGCGATGAGCGGCGCTGTGAACGCTGCGATAGCTTAGTCACCAA
GCGCGATTTGGATCAATGGTATTTCAAAATTACTTCATACGCTGATGAATTGCTGAATTTCTCAGAGTTGGATTGGCCTG
AGCGCATCACAACCATGCAGCGCAACTGGATTGGTCGCTCAGAAGGAGCTGAAATCAGCTTCAAATCTGAGGCTGGCGAC
CCAATTACGGTCTTTTCAACTCGCCCCGACACCTTGTGGGGTGCAACCTTTATGGTGTTGGCTCCGGAACATCCGTTGGT
TGCCAAATTGACCAGCGCCGAGCAAAAAGCCAGCGTCGATGCCTATGTAGCCGAAGCAATTCGCAAAACCGAAGTTGAGC
GCCAATCGACCGACGACGAAAAGCCCAAAACTGGCGTGTGGATCGGTGCGTATGCGATCAATCCAGCGAGCCAAGAGCGC
GTGCCAATTTGGATTGCCGATTATGTGTTGATGACCTATGGCACTGGCGCGATCATGGCCGTGCCCGGCCACGATGAACG
CGATTTTGCCTTTGCCAAAACCTTTGGCTTGGCAATCAAACGCGTAGTTACCCAATCTGAGCAAACTGCCGAAACTCCCT
TGGAAGCAGCCGAGCCAGCCTATGGCACGGTCGTTAATTCAGGCCAAATCGATGGCTTGAGCTCAGCCGAGGCCAAAGAA
GCTGTGATTAACTGGCTGGAAGCTGAGCAATTGGGCAAACGGGCGATTAATTATCGTTTGCGCGATTGGCTGGTCAGCCG
CCAACGCTATTGGGGTACGCCAATCCCGATGATTTATTGCCCAACATGTGGTACTGTACCCGTGCCAGAAGATCAATTAC
CATTGTTGCTGCCCGATAGTGTTGATTTCAAGCCAACAGGCGAATCGCCCTTGAAATTGCACCCAACCTGGCGCTTTACC
ACATGTCCAACCTGTGGCGGCGAGGCTGAGCGCGACACCGACACCATGGATACATTTGTTGATTCGTCGTGGTATCAAGT
GCGCTACTTAAGCCCGCACGAAACGAATGCGCCCTTTACCAAAGCGATTGCCGATAAATGGTTGCCAGTTGATCAATATA
CGGGTGGTCGCGAACATGCCGTGATGCACTTGCTGTACACCCGTTTTTGGTGGAAAGCCATGCGTGATATGGGCTTAGTC
AGCGCTAACGAGCCAATGACCCGTTTGATCAACCAAGGGGTGATTTTAGGCGAAGATAGTAATAAAATGTCGAAATCGCG
CGGCAATGTGATTGACCCAGATATGCTGGTAGCCCAATATGGTGCTGACACCGTGCGCACTTTCTTGATGTTTATTGGCC
CGTGGGAGCAAGGCGGTCCATGGAATAATCGCGGGATCGAAGGTTGTGTGCGCTTCTTGGATCGGGCTTGGCGGGTCGTG
ACTGATACGCCACAACGCCACGATGCTGTGGGCGATGCTAGCACATTGGAGCGCCAAACCCATCGGATCATCAAAAAAGT
TGGCGACGATTTGCAGCGTTTTGCCTTTAACACCGCCATTGCTGGCTTGATGGAGTTCGTCAACGAATTGATGAAAGTCC
GCGAAACCGATGTGTATGGCACTACAACATGGCGCAAGGCCACCGAAACCTTGACCTTGTTGTTAGCACCAATCGCTCCC
CACATTGCTGAAGAATTATGGGAATTTTTGGGCAACAGCCAAAGCGTCCATTTGCAAGCTTGGCCAAGCTACGATGAAAC
CTTATTGATCGACGAATCGATTGAATTGCCAGTGCAAATAAATGGTAAAGTTCGCGGTAAAATACAGGTAGCCGCCACCG
CCGATGAACCAAGCATCATTGCAACCGCTTTGGCTGATGAAAAGATTGCCCCCTTGGTCGCGGGCAAAACCATCGTCAAG
CAAATTGTGGTACCAAATCGCTTGGTCAATATCGTAATCAAATAA

Upstream 100 bases:

>100_bases
TCATGTTTAAAACCTATCCTTGACAGAAGCGAGCCAGGGGGTGAGGGGAAAAGTTTTAGATTTTGCCAATTGCAGATATT
TTTTAGCCAAGGAGTTGATG

Downstream 100 bases:

>100_bases
GTTTTTGGCCAGACAACGGAAGGGTCGTAGAGAAACCCTTCCGTTGGCCAACCCAAGAAGCGAGATAGCTTGGCAATTCG
GAAACGTTGCCAACATTCAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MATRYDSSITEPKWRERWERDGIYRFEPDSDKPKHYAVTMLPYPSGNLHIGHWYAMSPSDVHARYMRMRGYNVFFPMGFD
AFGLPAENAAIKNNLDPRKWTYSNIEYMRGQLQSMGMMVDWDQQIVSADPEYYRWNQWFFIQFFKRGLAYKKFSAVDWCP
KCNTTLAREQVVGDERRCERCDSLVTKRDLDQWYFKITSYADELLNFSELDWPERITTMQRNWIGRSEGAEISFKSEAGD
PITVFSTRPDTLWGATFMVLAPEHPLVAKLTSAEQKASVDAYVAEAIRKTEVERQSTDDEKPKTGVWIGAYAINPASQER
VPIWIADYVLMTYGTGAIMAVPGHDERDFAFAKTFGLAIKRVVTQSEQTAETPLEAAEPAYGTVVNSGQIDGLSSAEAKE
AVINWLEAEQLGKRAINYRLRDWLVSRQRYWGTPIPMIYCPTCGTVPVPEDQLPLLLPDSVDFKPTGESPLKLHPTWRFT
TCPTCGGEAERDTDTMDTFVDSSWYQVRYLSPHETNAPFTKAIADKWLPVDQYTGGREHAVMHLLYTRFWWKAMRDMGLV
SANEPMTRLINQGVILGEDSNKMSKSRGNVIDPDMLVAQYGADTVRTFLMFIGPWEQGGPWNNRGIEGCVRFLDRAWRVV
TDTPQRHDAVGDASTLERQTHRIIKKVGDDLQRFAFNTAIAGLMEFVNELMKVRETDVYGTTTWRKATETLTLLLAPIAP
HIAEELWEFLGNSQSVHLQAWPSYDETLLIDESIELPVQINGKVRGKIQVAATADEPSIIATALADEKIAPLVAGKTIVK
QIVVPNRLVNIVIK

Sequences:

>Translated_814_residues
MATRYDSSITEPKWRERWERDGIYRFEPDSDKPKHYAVTMLPYPSGNLHIGHWYAMSPSDVHARYMRMRGYNVFFPMGFD
AFGLPAENAAIKNNLDPRKWTYSNIEYMRGQLQSMGMMVDWDQQIVSADPEYYRWNQWFFIQFFKRGLAYKKFSAVDWCP
KCNTTLAREQVVGDERRCERCDSLVTKRDLDQWYFKITSYADELLNFSELDWPERITTMQRNWIGRSEGAEISFKSEAGD
PITVFSTRPDTLWGATFMVLAPEHPLVAKLTSAEQKASVDAYVAEAIRKTEVERQSTDDEKPKTGVWIGAYAINPASQER
VPIWIADYVLMTYGTGAIMAVPGHDERDFAFAKTFGLAIKRVVTQSEQTAETPLEAAEPAYGTVVNSGQIDGLSSAEAKE
AVINWLEAEQLGKRAINYRLRDWLVSRQRYWGTPIPMIYCPTCGTVPVPEDQLPLLLPDSVDFKPTGESPLKLHPTWRFT
TCPTCGGEAERDTDTMDTFVDSSWYQVRYLSPHETNAPFTKAIADKWLPVDQYTGGREHAVMHLLYTRFWWKAMRDMGLV
SANEPMTRLINQGVILGEDSNKMSKSRGNVIDPDMLVAQYGADTVRTFLMFIGPWEQGGPWNNRGIEGCVRFLDRAWRVV
TDTPQRHDAVGDASTLERQTHRIIKKVGDDLQRFAFNTAIAGLMEFVNELMKVRETDVYGTTTWRKATETLTLLLAPIAP
HIAEELWEFLGNSQSVHLQAWPSYDETLLIDESIELPVQINGKVRGKIQVAATADEPSIIATALADEKIAPLVAGKTIVK
QIVVPNRLVNIVIK
>Mature_813_residues
ATRYDSSITEPKWRERWERDGIYRFEPDSDKPKHYAVTMLPYPSGNLHIGHWYAMSPSDVHARYMRMRGYNVFFPMGFDA
FGLPAENAAIKNNLDPRKWTYSNIEYMRGQLQSMGMMVDWDQQIVSADPEYYRWNQWFFIQFFKRGLAYKKFSAVDWCPK
CNTTLAREQVVGDERRCERCDSLVTKRDLDQWYFKITSYADELLNFSELDWPERITTMQRNWIGRSEGAEISFKSEAGDP
ITVFSTRPDTLWGATFMVLAPEHPLVAKLTSAEQKASVDAYVAEAIRKTEVERQSTDDEKPKTGVWIGAYAINPASQERV
PIWIADYVLMTYGTGAIMAVPGHDERDFAFAKTFGLAIKRVVTQSEQTAETPLEAAEPAYGTVVNSGQIDGLSSAEAKEA
VINWLEAEQLGKRAINYRLRDWLVSRQRYWGTPIPMIYCPTCGTVPVPEDQLPLLLPDSVDFKPTGESPLKLHPTWRFTT
CPTCGGEAERDTDTMDTFVDSSWYQVRYLSPHETNAPFTKAIADKWLPVDQYTGGREHAVMHLLYTRFWWKAMRDMGLVS
ANEPMTRLINQGVILGEDSNKMSKSRGNVIDPDMLVAQYGADTVRTFLMFIGPWEQGGPWNNRGIEGCVRFLDRAWRVVT
DTPQRHDAVGDASTLERQTHRIIKKVGDDLQRFAFNTAIAGLMEFVNELMKVRETDVYGTTTWRKATETLTLLLAPIAPH
IAEELWEFLGNSQSVHLQAWPSYDETLLIDESIELPVQINGKVRGKIQVAATADEPSIIATALADEKIAPLVAGKTIVKQ
IVVPNRLVNIVIK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=886, Percent_Identity=33.8600451467269, Blast_Score=502, Evalue=1e-142,
Organism=Homo sapiens, GI46852147, Length=828, Percent_Identity=22.8260869565217, Blast_Score=91, Evalue=5e-18,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=41.9354838709677, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=383, Percent_Identity=21.9321148825065, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71997517, Length=814, Percent_Identity=28.7469287469287, Blast_Score=305, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI71997510, Length=814, Percent_Identity=28.7469287469287, Blast_Score=305, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI212645227, Length=381, Percent_Identity=28.0839895013123, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17510661, Length=433, Percent_Identity=21.0161662817552, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323414, Length=843, Percent_Identity=34.4009489916963, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321531, Length=676, Percent_Identity=21.4497041420118, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355409, Length=839, Percent_Identity=30.3933253873659, Blast_Score=374, Evalue=1e-103,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_HERA2 (A9AZ11)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547179.1
- ProteinModelPortal:   A9AZ11
- SMR:   A9AZ11
- GeneID:   5736270
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4419
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- BioCyc:   HAUR316274:HAUR_4419-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 92644; Mature: 92513

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 585-585

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRYDSSITEPKWRERWERDGIYRFEPDSDKPKHYAVTMLPYPSGNLHIGHWYAMSPSD
CCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEEEEEECCCCCEEEEEEEECCCHH
VHARYMRMRGYNVFFPMGFDAFGLPAENAAIKNNLDPRKWTYSNIEYMRGQLQSMGMMVD
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEECCHHHHHHHHHHHCCCEEE
WDQQIVSADPEYYRWNQWFFIQFFKRGLAYKKFSAVDWCPKCNTTLAREQVVGDERRCER
CCHHHHCCCCCCEECCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHH
CDSLVTKRDLDQWYFKITSYADELLNFSELDWPERITTMQRNWIGRSEGAEISFKSEAGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCC
PITVFSTRPDTLWGATFMVLAPEHPLVAKLTSAEQKASVDAYVAEAIRKTEVERQSTDDE
EEEEEECCCCCHHCCEEEEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPKTGVWIGAYAINPASQERVPIWIADYVLMTYGTGAIMAVPGHDERDFAFAKTFGLAIK
CCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
RVVTQSEQTAETPLEAAEPAYGTVVNSGQIDGLSSAEAKEAVINWLEAEQLGKRAINYRL
HHHHCCHHHHCCCHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDWLVSRQRYWGTPIPMIYCPTCGTVPVPEDQLPLLLPDSVDFKPTGESPLKLHPTWRFT
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEECCCEEEE
TCPTCGGEAERDTDTMDTFVDSSWYQVRYLSPHETNAPFTKAIADKWLPVDQYTGGREHA
ECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
VMHLLYTRFWWKAMRDMGLVSANEPMTRLINQGVILGEDSNKMSKSRGNVIDPDMLVAQY
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHHH
GADTVRTFLMFIGPWEQGGPWNNRGIEGCVRFLDRAWRVVTDTPQRHDAVGDASTLERQT
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHCCCCCHHHHHHHH
HRIIKKVGDDLQRFAFNTAIAGLMEFVNELMKVRETDVYGTTTWRKATETLTLLLAPIAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HIAEELWEFLGNSQSVHLQAWPSYDETLLIDESIELPVQINGKVRGKIQVAATADEPSII
HHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCEEECCEEEEEEEEEEECCCCCEE
ATALADEKIAPLVAGKTIVKQIVVPNRLVNIVIK
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
ATRYDSSITEPKWRERWERDGIYRFEPDSDKPKHYAVTMLPYPSGNLHIGHWYAMSPSD
CCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEEEEEECCCCCEEEEEEEECCCHH
VHARYMRMRGYNVFFPMGFDAFGLPAENAAIKNNLDPRKWTYSNIEYMRGQLQSMGMMVD
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEECCHHHHHHHHHHHCCCEEE
WDQQIVSADPEYYRWNQWFFIQFFKRGLAYKKFSAVDWCPKCNTTLAREQVVGDERRCER
CCHHHHCCCCCCEECCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHH
CDSLVTKRDLDQWYFKITSYADELLNFSELDWPERITTMQRNWIGRSEGAEISFKSEAGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCC
PITVFSTRPDTLWGATFMVLAPEHPLVAKLTSAEQKASVDAYVAEAIRKTEVERQSTDDE
EEEEEECCCCCHHCCEEEEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPKTGVWIGAYAINPASQERVPIWIADYVLMTYGTGAIMAVPGHDERDFAFAKTFGLAIK
CCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
RVVTQSEQTAETPLEAAEPAYGTVVNSGQIDGLSSAEAKEAVINWLEAEQLGKRAINYRL
HHHHCCHHHHCCCHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDWLVSRQRYWGTPIPMIYCPTCGTVPVPEDQLPLLLPDSVDFKPTGESPLKLHPTWRFT
HHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEECCCEEEE
TCPTCGGEAERDTDTMDTFVDSSWYQVRYLSPHETNAPFTKAIADKWLPVDQYTGGREHA
ECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
VMHLLYTRFWWKAMRDMGLVSANEPMTRLINQGVILGEDSNKMSKSRGNVIDPDMLVAQY
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEECCCCHHHHCCCCCCCHHHHHHHH
GADTVRTFLMFIGPWEQGGPWNNRGIEGCVRFLDRAWRVVTDTPQRHDAVGDASTLERQT
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHCCCCCHHHHHHHH
HRIIKKVGDDLQRFAFNTAIAGLMEFVNELMKVRETDVYGTTTWRKATETLTLLLAPIAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HIAEELWEFLGNSQSVHLQAWPSYDETLLIDESIELPVQINGKVRGKIQVAATADEPSII
HHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCEEECCEEEEEEEEEEECCCCCEE
ATALADEKIAPLVAGKTIVKQIVVPNRLVNIVIK
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA