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The map label for this gene is rnjA [H]

Identifier: 159900929

GI number: 159900929

Start: 5637760

End: 5639469

Strand: Direct

Name: rnjA [H]

Synonym: Haur_4416

Alternate gene names: 159900929

Gene position: 5637760-5639469 (Clockwise)

Preceding gene: 159900928

Following gene: 159900932

Centisome position: 88.83

GC content: 51.29

Gene sequence:

>1710_bases
ATGACTAAAAACAAATTACGGGTTATCCCACTTGGGGGCGCATCGGAAGTTGGCCGCAACATGTGGGTTTTTGAGTATGA
AGAAGATATTGTCATCTGCGACATGGGCGTGATGTTTCCCGAAAGCGAGATGATGGGCGTTGATTTGGTGTTGCCCGACA
TCACCTATTTGCGCGATAAAGTTGCGAATATTCGCGGTATTTTGCTGACCCACGGCCACGAAGACCACATCGGAGCCGTG
CCTTGGTTGATTGGCGATCTAGGCTTTCCAACAGTGTATGGCACAACATTAACCCTTGGCTTGCTCTCGAATAAACTCAA
AGAACATCGGCTAACAGATAAAGTTGCAACCAAAGTCATTGCGCCAGGCGATAAATTCCAGCTTGGGTGCTTCACGATTG
AGCCATTTCACATTGCCCACTCCATCCCTGATTCAGTCGGCTTTGGCTTGACCTCGCCTGCTGGTTTGGCCTTGTACATC
ACCGACTGGAAGCTTGACCATACCCCCGTTGACAATTGGCCAACCGAAATTGATCGGTTGGTTGATTTTGGCCGCCGCAA
CCCAATCGTGTTGGTTACCGACTGCGTGCGGGTTGAATCGAAAGGCTATACCCCATCGGAGCGAGTGGTGGGTGATGCCT
TTGATCAAATTTTTGCCACAGCTCCTGGGCGAGTCATTATGGCCACCTTTGCCTCAAATATCTCACGGGTGCAAATGGCA
ATTAATGCAGCGATTGATTATGGTCGCAAAGTTGTGCCAGTTGGTCGCTCGATGGAAAACAACACCTCGATCGCCCGTGA
ATTGGGCTACCTTGAAGTGCCTGATGGTACGTTGATTCGAGCAGGCGATATGAAGAACTTGCCTGATGATCAAATTGCGA
TTGTCTGTACGGGCAGCCAAGGCGAGCCAATGGCGGCGCTCTCACGCATGGCCAATCGCGACCATCGCCAAATTTCAATT
CAACGCGGCGATACCGTGGTTATCTCAGCCTCGCCAATTCCAGGCAACGAAGTATCGGTCTATAAAGTGATCGATAATTT
GTACAAACTTGGGGCAAATGTGATCTATGGTTCAGATTTGCCAGTGCATGTTTCTGGGCACGCCGCTCAAGAAGAGCTGA
AAATGTTGCTCAACTTGGTGCAACCACAGTATGTTATTCCCTTCCACGGCGATTATCGTCACTTGGCCCGCTATAAGCAA
TTGGCCGAGGGAATGGGCTTTAACGGCGATAACGTGCTAATTGCCGAGGGCGGCTCGATTATGGAATTCAGCCAAAATTA
CGGCAAACTGGTTAGCCGCGCTCCCGTCGGCCATGTCTTTGTTGATGGCGTTTCGGTGGGCGATATTGGTGGGGCTGTGG
TGCGCGACCGCCAAATGCTTTCCAAAGATGGCATCCTGATGGTTGTCGTTTCGGTCGATCTTCAAACTGGCAACTTGGTG
GCTGGGCCAGATATTGTGAGCCGCGGCTTTGTTTCGACCCGCGATTCAACCGATTTGATCGAGGGAGCCAAAGAAGCGGT
GCGAGCCTCGTTCAGCGAAAGCTATGTGGTGCCAGCACGGGCATTTAGCGATGGTGAAGAAACCGCCGAAGGCAACTCGC
CAGCAGTTTCCTCGCCAGCAGCTCGTAAAATCAAAGAAGTGGTTGGGCGTTTCCTCTACGAACGTACCCGCCGCCGCCCA
ATGGTTATTCCGGTGGTGATGGAAGTTTAA

Upstream 100 bases:

>100_bases
AATTCTATTTAACAACTATCTCAGCCAAACGGGGTTGGGATTATTCAGAAAGGAGCCATGACGACAGTGCAGGCCTACTT
GCACTGTGGTTTGTATTCGT

Downstream 100 bases:

>100_bases
CTACTACAAGCAAAGGCCGCCTTGGAAATGCTCCAAGGCGGCCTTTGCTTTAACCAATTAAATGGCAGTTACTGAAAAAT
CTCAGTAACTGCCAACTTGT

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTKNKLRVIPLGGASEVGRNMWVFEYEEDIVICDMGVMFPESEMMGVDLVLPDITYLRDKVANIRGILLTHGHEDHIGAV
PWLIGDLGFPTVYGTTLTLGLLSNKLKEHRLTDKVATKVIAPGDKFQLGCFTIEPFHIAHSIPDSVGFGLTSPAGLALYI
TDWKLDHTPVDNWPTEIDRLVDFGRRNPIVLVTDCVRVESKGYTPSERVVGDAFDQIFATAPGRVIMATFASNISRVQMA
INAAIDYGRKVVPVGRSMENNTSIARELGYLEVPDGTLIRAGDMKNLPDDQIAIVCTGSQGEPMAALSRMANRDHRQISI
QRGDTVVISASPIPGNEVSVYKVIDNLYKLGANVIYGSDLPVHVSGHAAQEELKMLLNLVQPQYVIPFHGDYRHLARYKQ
LAEGMGFNGDNVLIAEGGSIMEFSQNYGKLVSRAPVGHVFVDGVSVGDIGGAVVRDRQMLSKDGILMVVVSVDLQTGNLV
AGPDIVSRGFVSTRDSTDLIEGAKEAVRASFSESYVVPARAFSDGEETAEGNSPAVSSPAARKIKEVVGRFLYERTRRRP
MVIPVVMEV

Sequences:

>Translated_569_residues
MTKNKLRVIPLGGASEVGRNMWVFEYEEDIVICDMGVMFPESEMMGVDLVLPDITYLRDKVANIRGILLTHGHEDHIGAV
PWLIGDLGFPTVYGTTLTLGLLSNKLKEHRLTDKVATKVIAPGDKFQLGCFTIEPFHIAHSIPDSVGFGLTSPAGLALYI
TDWKLDHTPVDNWPTEIDRLVDFGRRNPIVLVTDCVRVESKGYTPSERVVGDAFDQIFATAPGRVIMATFASNISRVQMA
INAAIDYGRKVVPVGRSMENNTSIARELGYLEVPDGTLIRAGDMKNLPDDQIAIVCTGSQGEPMAALSRMANRDHRQISI
QRGDTVVISASPIPGNEVSVYKVIDNLYKLGANVIYGSDLPVHVSGHAAQEELKMLLNLVQPQYVIPFHGDYRHLARYKQ
LAEGMGFNGDNVLIAEGGSIMEFSQNYGKLVSRAPVGHVFVDGVSVGDIGGAVVRDRQMLSKDGILMVVVSVDLQTGNLV
AGPDIVSRGFVSTRDSTDLIEGAKEAVRASFSESYVVPARAFSDGEETAEGNSPAVSSPAARKIKEVVGRFLYERTRRRP
MVIPVVMEV
>Mature_568_residues
TKNKLRVIPLGGASEVGRNMWVFEYEEDIVICDMGVMFPESEMMGVDLVLPDITYLRDKVANIRGILLTHGHEDHIGAVP
WLIGDLGFPTVYGTTLTLGLLSNKLKEHRLTDKVATKVIAPGDKFQLGCFTIEPFHIAHSIPDSVGFGLTSPAGLALYIT
DWKLDHTPVDNWPTEIDRLVDFGRRNPIVLVTDCVRVESKGYTPSERVVGDAFDQIFATAPGRVIMATFASNISRVQMAI
NAAIDYGRKVVPVGRSMENNTSIARELGYLEVPDGTLIRAGDMKNLPDDQIAIVCTGSQGEPMAALSRMANRDHRQISIQ
RGDTVVISASPIPGNEVSVYKVIDNLYKLGANVIYGSDLPVHVSGHAAQEELKMLLNLVQPQYVIPFHGDYRHLARYKQL
AEGMGFNGDNVLIAEGGSIMEFSQNYGKLVSRAPVGHVFVDGVSVGDIGGAVVRDRQMLSKDGILMVVVSVDLQTGNLVA
GPDIVSRGFVSTRDSTDLIEGAKEAVRASFSESYVVPARAFSDGEETAEGNSPAVSSPAARKIKEVVGRFLYERTRRRPM
VIPVVMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62036; Mature: 61905

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNKLRVIPLGGASEVGRNMWVFEYEEDIVICDMGVMFPESEMMGVDLVLPDITYLRDK
CCCCCEEEEECCCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHH
VANIRGILLTHGHEDHIGAVPWLIGDLGFPTVYGTTLTLGLLSNKLKEHRLTDKVATKVI
HHCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGDKFQLGCFTIEPFHIAHSIPDSVGFGLTSPAGLALYITDWKLDHTPVDNWPTEIDRL
CCCCCEEEEEEEECHHHHHHCCCHHCCCCCCCCCCEEEEEEECEECCCCCCCCHHHHHHH
VDFGRRNPIVLVTDCVRVESKGYTPSERVVGDAFDQIFATAPGRVIMATFASNISRVQMA
HHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCCHHHHHHH
INAAIDYGRKVVPVGRSMENNTSIARELGYLEVPDGTLIRAGDMKNLPDDQIAIVCTGSQ
HHHHHHCCCEEEECCCCCCCCHHHHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEECCC
GEPMAALSRMANRDHRQISIQRGDTVVISASPIPGNEVSVYKVIDNLYKLGANVIYGSDL
CCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCCC
PVHVSGHAAQEELKMLLNLVQPQYVIPFHGDYRHLARYKQLAEGMGFNGDNVLIAEGGSI
CEEECCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCH
MEFSQNYGKLVSRAPVGHVFVDGVSVGDIGGAVVRDRQMLSKDGILMVVVSVDLQTGNLV
HHHHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCEE
AGPDIVSRGFVSTRDSTDLIEGAKEAVRASFSESYVVPARAFSDGEETAEGNSPAVSSPA
ECCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCCCCHH
ARKIKEVVGRFLYERTRRRPMVIPVVMEV
HHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure 
TKNKLRVIPLGGASEVGRNMWVFEYEEDIVICDMGVMFPESEMMGVDLVLPDITYLRDK
CCCCEEEEECCCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHH
VANIRGILLTHGHEDHIGAVPWLIGDLGFPTVYGTTLTLGLLSNKLKEHRLTDKVATKVI
HHCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
APGDKFQLGCFTIEPFHIAHSIPDSVGFGLTSPAGLALYITDWKLDHTPVDNWPTEIDRL
CCCCCEEEEEEEECHHHHHHCCCHHCCCCCCCCCCEEEEEEECEECCCCCCCCHHHHHHH
VDFGRRNPIVLVTDCVRVESKGYTPSERVVGDAFDQIFATAPGRVIMATFASNISRVQMA
HHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHCCHHHHHHH
INAAIDYGRKVVPVGRSMENNTSIARELGYLEVPDGTLIRAGDMKNLPDDQIAIVCTGSQ
HHHHHHCCCEEEECCCCCCCCHHHHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEECCC
GEPMAALSRMANRDHRQISIQRGDTVVISASPIPGNEVSVYKVIDNLYKLGANVIYGSDL
CCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCCC
PVHVSGHAAQEELKMLLNLVQPQYVIPFHGDYRHLARYKQLAEGMGFNGDNVLIAEGGSI
CEEECCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCH
MEFSQNYGKLVSRAPVGHVFVDGVSVGDIGGAVVRDRQMLSKDGILMVVVSVDLQTGNLV
HHHHHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCEE
AGPDIVSRGFVSTRDSTDLIEGAKEAVRASFSESYVVPARAFSDGEETAEGNSPAVSSPA
ECCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCCCCHH
ARKIKEVVGRFLYERTRRRPMVIPVVMEV
HHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]