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The map label for this gene is pelA [H]

Identifier: 159899978

GI number: 159899978

Start: 4355100

End: 4356560

Strand: Direct

Name: pelA [H]

Synonym: Haur_3461

Alternate gene names: 159899978

Gene position: 4355100-4356560 (Clockwise)

Preceding gene: 159899971

Following gene: 159899979

Centisome position: 68.62

GC content: 52.36

Gene sequence:

>1461_bases
ATGAAATTATCAATTGCCCGACGTTGGGTTATTGCCACCGGCTTAGCCACTGCCTTGATTGGCGCAATCCAAAGCCCAAT
CACCAACGCCCAAACTGGCCTAAGCTGCCAAGTCAATTATACGCTTACTAACCAATGGGGCAGCGGGTTTCAAGCCGATG
TGGTGGTGCGCAACACCGGAACCAGTGTGATCAACGGCTGGACGGTTGCTTGGAGTGCTGCCAGCGGCCAGCAAATTGGC
CAAATGTGGAATGCGACGTTTACCCAAAGTGGCAGCCAAGTCAGTGCCAAAAATGTTGATTGGAACGCCAGCATCGCTGC
TGGTGGCAGCCAAAGCTTTGGCTTTACCGCTACCACGACTGGTAGCTTGGCCGTGCCCAGCAGCTTTACGGTCAATGGCG
TTGTTTGTGGCGGTAGTGTTAGCCCAACCGCAACCCGCACGCCAGCCGCGACTGCCACGCGTACTCCAATTGCCACTGCA
ACCCGCACGCCAGCTGTAACCGCCACGCGCACCCCTGTGGCAACTACTACCCGTACTCCAATTGCCACGGCTACCGTCGT
TCCAACCAATCCACCAGTCAGCAATGGCTTGATTGGCTGGGCCACGGTTGCTGGTTCGGGCTTAAGCACAACTACTGGCG
GCACTGGTGGTAGCACAGTTACCGCAGCCAACTTTACTGAATTGCAAAACTACGCCAAATCGTCATCGCCCATGATTATC
AAGTTCTCGGGTACGATGCAAGGCACACTGACGGTTGCCTCGAACAAAACGATTATCGGCAGCAATGGAGCCTTGATCCA
AGGTAATGTCAAAATCTCAGGCGCTCAAAATATTATTTTGCAAAATTTTGCGATCAACGGCAATAGCTGCTCAAGCTACG
ATAACTGCCGCGCTGGAAGCGATGCCTTGGGGATTAGCAATTCGCACCATATTTGGGCCGACCACTTGACGATTACCAAT
GGCCAAGATGGCAATTTCGACATTAACAATGGCTCTGATTTCATTACGGTTTCGTGGAGCAAATTCGGCTATACCACCAA
CAAAGAGCATCGTTTCTCGAACTTGATTGGTAGCTCAGACGATGCAGCCTCGACCGATAGCGGTAAATTGAACGTGACCT
TCCATCATAACTGGTGGTTTGGTGGGGCAATGCAGCGCATGCCACGTACGCGCTTCGGCAAAATTCACGTATTCAATAAT
TTGTACACCACCACTGGCAACGATTATTGTGTTAGCTCAGGCTATCAATCCAAAGTGTTGCTCGAAAATAATGCCTTCAT
TGGGGTCAACACGCCGCACCGCTTGCACGATGGCGATCTCAAGGCGGTGGGCAATCTCTACCAAAACACCAGCGGCGATC
AAATTAGTACTGGCGTTGCCTTCACGCCGCCCTACAGCTATAGCGCCGAAGCTGCTAGCTCACTCAGCAGTTCAGTCCAA
GCTGGCGCAGGAGCGAAGTAG

Upstream 100 bases:

>100_bases
CAGGGTCTAGAATTCAGGGCTATGTTCGCTGCTGTTTGTTCTCTCATTTCCCGATCCCTAACAACCGATCCCCAACAGCC
AGTTTACAAGGAGTTTACCA

Downstream 100 bases:

>100_bases
CATATTAGGGGCTGGGGATCGGGAAATAGAACATAGAGCATAGAACATAGAACATTGTGAATATGGCTATTGGCTATCGG
AATATGCTTGGAATTCCAAT

Product: cellulose-binding family II protein

Products: NA

Alternate protein names: Pectate lyase A; PelA [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKLSIARRWVIATGLATALIGAIQSPITNAQTGLSCQVNYTLTNQWGSGFQADVVVRNTGTSVINGWTVAWSAASGQQIG
QMWNATFTQSGSQVSAKNVDWNASIAAGGSQSFGFTATTTGSLAVPSSFTVNGVVCGGSVSPTATRTPAATATRTPIATA
TRTPAVTATRTPVATTTRTPIATATVVPTNPPVSNGLIGWATVAGSGLSTTTGGTGGSTVTAANFTELQNYAKSSSPMII
KFSGTMQGTLTVASNKTIIGSNGALIQGNVKISGAQNIILQNFAINGNSCSSYDNCRAGSDALGISNSHHIWADHLTITN
GQDGNFDINNGSDFITVSWSKFGYTTNKEHRFSNLIGSSDDAASTDSGKLNVTFHHNWWFGGAMQRMPRTRFGKIHVFNN
LYTTTGNDYCVSSGYQSKVLLENNAFIGVNTPHRLHDGDLKAVGNLYQNTSGDQISTGVAFTPPYSYSAEAASSLSSSVQ
AGAGAK

Sequences:

>Translated_486_residues
MKLSIARRWVIATGLATALIGAIQSPITNAQTGLSCQVNYTLTNQWGSGFQADVVVRNTGTSVINGWTVAWSAASGQQIG
QMWNATFTQSGSQVSAKNVDWNASIAAGGSQSFGFTATTTGSLAVPSSFTVNGVVCGGSVSPTATRTPAATATRTPIATA
TRTPAVTATRTPVATTTRTPIATATVVPTNPPVSNGLIGWATVAGSGLSTTTGGTGGSTVTAANFTELQNYAKSSSPMII
KFSGTMQGTLTVASNKTIIGSNGALIQGNVKISGAQNIILQNFAINGNSCSSYDNCRAGSDALGISNSHHIWADHLTITN
GQDGNFDINNGSDFITVSWSKFGYTTNKEHRFSNLIGSSDDAASTDSGKLNVTFHHNWWFGGAMQRMPRTRFGKIHVFNN
LYTTTGNDYCVSSGYQSKVLLENNAFIGVNTPHRLHDGDLKAVGNLYQNTSGDQISTGVAFTPPYSYSAEAASSLSSSVQ
AGAGAK
>Mature_486_residues
MKLSIARRWVIATGLATALIGAIQSPITNAQTGLSCQVNYTLTNQWGSGFQADVVVRNTGTSVINGWTVAWSAASGQQIG
QMWNATFTQSGSQVSAKNVDWNASIAAGGSQSFGFTATTTGSLAVPSSFTVNGVVCGGSVSPTATRTPAATATRTPIATA
TRTPAVTATRTPVATTTRTPIATATVVPTNPPVSNGLIGWATVAGSGLSTTTGGTGGSTVTAANFTELQNYAKSSSPMII
KFSGTMQGTLTVASNKTIIGSNGALIQGNVKISGAQNIILQNFAINGNSCSSYDNCRAGSDALGISNSHHIWADHLTITN
GQDGNFDINNGSDFITVSWSKFGYTTNKEHRFSNLIGSSDDAASTDSGKLNVTFHHNWWFGGAMQRMPRTRFGKIHVFNN
LYTTTGNDYCVSSGYQSKVLLENNAFIGVNTPHRLHDGDLKAVGNLYQNTSGDQISTGVAFTPPYSYSAEAASSLSSSVQ
AGAGAK

Specific function: Cleaves unsaturated trigalacturonate from pectin. Activity is highest towards polygalacturonic acid, activity on methylated pectins decreases with an increasing degree of methylation [H]

COG id: COG3866

COG function: function code G; Pectate lyase

Gene ontology:

Cell location: Secreted, extracellular space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002022
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00544 Pec_lyase_C [H]

EC number: =4.2.2.22 [H]

Molecular weight: Translated: 50466; Mature: 50466

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00561 CBD_BACTERIAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSIARRWVIATGLATALIGAIQSPITNAQTGLSCQVNYTLTNQWGSGFQADVVVRNTG
CCCCHHHHEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEECC
TSVINGWTVAWSAASGQQIGQMWNATFTQSGSQVSAKNVDWNASIAAGGSQSFGFTATTT
CCEEECEEEEEECCCCHHHHHHHCCCEECCCCEEEECCCCCCEEEECCCCCCCCEEEECC
GSLAVPSSFTVNGVVCGGSVSPTATRTPAATATRTPIATATRTPAVTATRTPVATTTRTP
CCEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCC
IATATVVPTNPPVSNGLIGWATVAGSGLSTTTGGTGGSTVTAANFTELQNYAKSSSPMII
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEE
KFSGTMQGTLTVASNKTIIGSNGALIQGNVKISGAQNIILQNFAINGNSCSSYDNCRAGS
EECCCCEEEEEEECCCEEEECCCCEEECCEEECCCCEEEEEEEEECCCCCCCCCCCCCCC
DALGISNSHHIWADHLTITNGQDGNFDINNGSDFITVSWSKFGYTTNKEHRFSNLIGSSD
CCCCCCCCCEEEEEEEEEECCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHCCCCC
DAASTDSGKLNVTFHHNWWFGGAMQRMPRTRFGKIHVFNNLYTTTGNDYCVSSGYQSKVL
CCCCCCCCEEEEEEEECEEECHHHHHCCCCCCCEEEEEECEEEECCCCEEECCCCCCEEE
LENNAFIGVNTPHRLHDGDLKAVGNLYQNTSGDQISTGVAFTPPYSYSAEAASSLSSSVQ
EECCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEECCCCCCCHHHHHHHHHHHC
AGAGAK
CCCCCC
>Mature Secondary Structure
MKLSIARRWVIATGLATALIGAIQSPITNAQTGLSCQVNYTLTNQWGSGFQADVVVRNTG
CCCCHHHHEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEECC
TSVINGWTVAWSAASGQQIGQMWNATFTQSGSQVSAKNVDWNASIAAGGSQSFGFTATTT
CCEEECEEEEEECCCCHHHHHHHCCCEECCCCEEEECCCCCCEEEECCCCCCCCEEEECC
GSLAVPSSFTVNGVVCGGSVSPTATRTPAATATRTPIATATRTPAVTATRTPVATTTRTP
CCEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCC
IATATVVPTNPPVSNGLIGWATVAGSGLSTTTGGTGGSTVTAANFTELQNYAKSSSPMII
CEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEE
KFSGTMQGTLTVASNKTIIGSNGALIQGNVKISGAQNIILQNFAINGNSCSSYDNCRAGS
EECCCCEEEEEEECCCEEEECCCCEEECCEEECCCCEEEEEEEEECCCCCCCCCCCCCCC
DALGISNSHHIWADHLTITNGQDGNFDINNGSDFITVSWSKFGYTTNKEHRFSNLIGSSD
CCCCCCCCCEEEEEEEEEECCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHCCCCC
DAASTDSGKLNVTFHHNWWFGGAMQRMPRTRFGKIHVFNNLYTTTGNDYCVSSGYQSKVL
CCCCCCCCEEEEEEEECEEECHHHHHCCCCCCCEEEEEECEEEECCCCEEECCCCCCEEE
LENNAFIGVNTPHRLHDGDLKAVGNLYQNTSGDQISTGVAFTPPYSYSAEAASSLSSSVQ
EECCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEECCCCCCCHHHHHHHHHHHC
AGAGAK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]