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The map label for this gene is lepA [H]

Identifier: 159899708

GI number: 159899708

Start: 4035290

End: 4037092

Strand: Direct

Name: lepA [H]

Synonym: Haur_3190

Alternate gene names: 159899708

Gene position: 4035290-4037092 (Clockwise)

Preceding gene: 159899707

Following gene: 159899711

Centisome position: 63.58

GC content: 51.03

Gene sequence:

>1803_bases
ATGGCTGAACAGACGCACATTCGCAACTTTAGCATTATCGCCCACGTCGATCATGGCAAAACCACGCTTTCGGATCGTTT
GTTGGAAGTGACCCAAACACTTTCTTCACGCGAAATGCGGGCACAAACCCTCGATGCCATGGATTTGGAACGCGAAAAGG
GCATCACGATTAAGATGCACCCCGTCCGGCTAGAATATACCGCCAAGGATGGTCAACAATATGCCTTGCATATGATCGAC
TGCCCAGGCCACGTTGACTTTAGCTACGAAGTTTCACGCTCGTTGCAAGCCTGTGAAGGCGCATTGTTGATCGTTGATGC
TTCCCAAGGGATCGAGGCCCAAACATTGGCCAACGTCTACTTGGCACTCGAAAACAATCTCGAAATTATTCCAATCTTGA
ATAAAATCGACTTGCCTGGCGCTGAAGTCGAGCGCGTTTCCGAGGAAGTCGAGCATGTGCTGGGCTTGCCACGCGAAGAG
ATTATTTTGGCTTCGGGCAAGGAAGGCACAGGCGTGCCCGAAATTCTCGAAGCGATTGTCGCCCGCATTCCACCACCCAA
AGGCATCAACGAAGCGCCTGCTCGTGCCTTGATTTTCGATTCGCAATACAACGCCTACAAAGGCGTAATTGCCTATATTC
GGGTGGTCGATGGCACGATTCGAGCTGGCGATAAATTGCGCCTAATGGGCACCGAAGTGCTGACCGAAGCCATGGAAATT
GGCTTCTTCCGACCAGGCATGGTCGCTGGCCAAGCGCTCAGCGCTGGCGAAGTCGGCTATATCGCGACTGGTTTGAAATC
GGTGCGCGATTGCCAAGTTGGCGATACAATCACGCTCGTTGATAATCCTGCAAAAACCTCACTGGCCCCGTATGAGCCAG
CCAAGCCAATGGTGTTTGCCGGTTTGTATCCCGTCGATTCAGGCGATTACACCTTGCTGCGCGATGCGCTGGACAAATTG
CGTTTGAACGATGCGGCGCTTTCGTTCGAGCCAGAAACCTCAGCCGCGCTAGGATTTGGCTTCCGTGCGGGCTTCTTGGG
CTTGCTGCACATGGAAATTGTGCAAGAGCGGCTCGAACGTGAATACAACATCGATCTATTGGTAACTGCGCCCAGCGTGG
AATATCAAGTGCTAACCAATGCTGGCGAAATTTTGATCATCGATAATCCCTCAGAATTGCCCGATGTTGGCCAAATCAGC
CAGATCGAAGAGCCGATGATGTCGATTAGCATTATCGTACCAACTCGCTATATTGGCGTGGTCATGGAGTTGGTCACGGG
CAAACGTGGTATTTTCCAAAGCATGGATTATCTCGATAAAGAGCGGGTCGTGCTGAAGTACGAAATTCCACTCTCAGAAA
TTGTGATCGATTTCTATGATTCGCTTAAATCGCGCACCCAAGGCTATGCTTCGCTGGATTATCATCTGAGCAGCTATCGC
GCCTCGGATTTGGTTAAGCTTGACGTGTTGGTCAATGGTACGGCGGTTGATGCGCTTTCGATGATCACCCACCGCGACAA
TGCCTATCGCCAAGGCCGCCAATTGGTCGAAAAGCTGCAAAAGCTGATTCCACGCCAAATGTTTGAAGTGCCAATTCAGG
CAGCAGTTGGATCACGGGTGATCGCCCGTGAAACCATTCGAGCAATGCGCAAAGACGTGTTGTCGAAATGTTATGGTGGC
GACATCACCCGTAAGCGCAAGCTCTTGGAAAAACAAAAAGAGGGCAAGAAGCGCATGAAGATGGTTGGCAACGTCGAGAT
TCCACAAGATGCCTTTATGGCGGTTTTGAAGCTCGATAGCTAA

Upstream 100 bases:

>100_bases
AATCGCCATTCAGCCTTAGCTCAATCAATTTCGTGCCTCATGATATACTGTCAACAGATTGACATTGCAAGCCCTCACAC
AATAGGACATAAAGGACACA

Downstream 100 bases:

>100_bases
TGTAGGTTCCTTAATCCTTTTCTTTCAAGGGGAAGGTTTTAAGGCTTGTATTCATCAATCACCACCCTTCCGTCCCGCCT
CAAGGCGGGAAACGCAGGGG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MAEQTHIRNFSIIAHVDHGKTTLSDRLLEVTQTLSSREMRAQTLDAMDLEREKGITIKMHPVRLEYTAKDGQQYALHMID
CPGHVDFSYEVSRSLQACEGALLIVDASQGIEAQTLANVYLALENNLEIIPILNKIDLPGAEVERVSEEVEHVLGLPREE
IILASGKEGTGVPEILEAIVARIPPPKGINEAPARALIFDSQYNAYKGVIAYIRVVDGTIRAGDKLRLMGTEVLTEAMEI
GFFRPGMVAGQALSAGEVGYIATGLKSVRDCQVGDTITLVDNPAKTSLAPYEPAKPMVFAGLYPVDSGDYTLLRDALDKL
RLNDAALSFEPETSAALGFGFRAGFLGLLHMEIVQERLEREYNIDLLVTAPSVEYQVLTNAGEILIIDNPSELPDVGQIS
QIEEPMMSISIIVPTRYIGVVMELVTGKRGIFQSMDYLDKERVVLKYEIPLSEIVIDFYDSLKSRTQGYASLDYHLSSYR
ASDLVKLDVLVNGTAVDALSMITHRDNAYRQGRQLVEKLQKLIPRQMFEVPIQAAVGSRVIARETIRAMRKDVLSKCYGG
DITRKRKLLEKQKEGKKRMKMVGNVEIPQDAFMAVLKLDS

Sequences:

>Translated_600_residues
MAEQTHIRNFSIIAHVDHGKTTLSDRLLEVTQTLSSREMRAQTLDAMDLEREKGITIKMHPVRLEYTAKDGQQYALHMID
CPGHVDFSYEVSRSLQACEGALLIVDASQGIEAQTLANVYLALENNLEIIPILNKIDLPGAEVERVSEEVEHVLGLPREE
IILASGKEGTGVPEILEAIVARIPPPKGINEAPARALIFDSQYNAYKGVIAYIRVVDGTIRAGDKLRLMGTEVLTEAMEI
GFFRPGMVAGQALSAGEVGYIATGLKSVRDCQVGDTITLVDNPAKTSLAPYEPAKPMVFAGLYPVDSGDYTLLRDALDKL
RLNDAALSFEPETSAALGFGFRAGFLGLLHMEIVQERLEREYNIDLLVTAPSVEYQVLTNAGEILIIDNPSELPDVGQIS
QIEEPMMSISIIVPTRYIGVVMELVTGKRGIFQSMDYLDKERVVLKYEIPLSEIVIDFYDSLKSRTQGYASLDYHLSSYR
ASDLVKLDVLVNGTAVDALSMITHRDNAYRQGRQLVEKLQKLIPRQMFEVPIQAAVGSRVIARETIRAMRKDVLSKCYGG
DITRKRKLLEKQKEGKKRMKMVGNVEIPQDAFMAVLKLDS
>Mature_599_residues
AEQTHIRNFSIIAHVDHGKTTLSDRLLEVTQTLSSREMRAQTLDAMDLEREKGITIKMHPVRLEYTAKDGQQYALHMIDC
PGHVDFSYEVSRSLQACEGALLIVDASQGIEAQTLANVYLALENNLEIIPILNKIDLPGAEVERVSEEVEHVLGLPREEI
ILASGKEGTGVPEILEAIVARIPPPKGINEAPARALIFDSQYNAYKGVIAYIRVVDGTIRAGDKLRLMGTEVLTEAMEIG
FFRPGMVAGQALSAGEVGYIATGLKSVRDCQVGDTITLVDNPAKTSLAPYEPAKPMVFAGLYPVDSGDYTLLRDALDKLR
LNDAALSFEPETSAALGFGFRAGFLGLLHMEIVQERLEREYNIDLLVTAPSVEYQVLTNAGEILIIDNPSELPDVGQISQ
IEEPMMSISIIVPTRYIGVVMELVTGKRGIFQSMDYLDKERVVLKYEIPLSEIVIDFYDSLKSRTQGYASLDYHLSSYRA
SDLVKLDVLVNGTAVDALSMITHRDNAYRQGRQLVEKLQKLIPRQMFEVPIQAAVGSRVIARETIRAMRKDVLSKCYGGD
ITRKRKLLEKQKEGKKRMKMVGNVEIPQDAFMAVLKLDS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=48.5903814262023, Blast_Score=626, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=41.3533834586466, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI25306283, Length=151, Percent_Identity=39.7350993377483, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=151, Percent_Identity=39.7350993377483, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=151, Percent_Identity=39.7350993377483, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=36.9127516778523, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI18390331, Length=179, Percent_Identity=32.4022346368715, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=39.0909090909091, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=39.0909090909091, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=39.0909090909091, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=35.0746268656716, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=35.0746268656716, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=56.1551433389545, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=28.3168316831683, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789738, Length=187, Percent_Identity=33.6898395721925, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789559, Length=247, Percent_Identity=27.5303643724696, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790835, Length=154, Percent_Identity=29.2207792207792, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.6666666666667, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17533571, Length=141, Percent_Identity=36.8794326241135, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=34.1935483870968, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=145, Percent_Identity=37.2413793103448, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=145, Percent_Identity=37.2413793103448, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=174, Percent_Identity=32.183908045977, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=250, Percent_Identity=30.8, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=45.819397993311, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6323098, Length=170, Percent_Identity=34.7058823529412, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.255033557047, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.255033557047, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=140, Percent_Identity=33.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=38.2978723404255, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=283, Percent_Identity=27.208480565371, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=45.0751252086811, Blast_Score=537, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24582462, Length=147, Percent_Identity=39.4557823129252, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=40.1459854014599, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=166, Percent_Identity=31.9277108433735, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24585713, Length=166, Percent_Identity=31.9277108433735, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24585709, Length=166, Percent_Identity=31.9277108433735, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=34.0740740740741, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI221458488, Length=153, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=228, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921738, Length=280, Percent_Identity=29.2857142857143, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66528; Mature: 66397

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTHIRNFSIIAHVDHGKTTLSDRLLEVTQTLSSREMRAQTLDAMDLEREKGITIKMH
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PVRLEYTAKDGQQYALHMIDCPGHVDFSYEVSRSLQACEGALLIVDASQGIEAQTLANVY
EEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHEEE
LALENNLEIIPILNKIDLPGAEVERVSEEVEHVLGLPREEIILASGKEGTGVPEILEAIV
EEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHHHHHH
ARIPPPKGINEAPARALIFDSQYNAYKGVIAYIRVVDGTIRAGDKLRLMGTEVLTEAMEI
HHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHH
GFFRPGMVAGQALSAGEVGYIATGLKSVRDCQVGDTITLVDNPAKTSLAPYEPAKPMVFA
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
GLYPVDSGDYTLLRDALDKLRLNDAALSFEPETSAALGFGFRAGFLGLLHMEIVQERLER
EEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
EYNIDLLVTAPSVEYQVLTNAGEILIIDNPSELPDVGQISQIEEPMMSISIIVPTRYIGV
HCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHCCHHEEEEEECHHHHHH
VMELVTGKRGIFQSMDYLDKERVVLKYEIPLSEIVIDFYDSLKSRTQGYASLDYHLSSYR
HHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
ASDLVKLDVLVNGTAVDALSMITHRDNAYRQGRQLVEKLQKLIPRQMFEVPIQAAVGSRV
CCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHH
IARETIRAMRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKMVGNVEIPQDAFMAVLKLDS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECC
>Mature Secondary Structure 
AEQTHIRNFSIIAHVDHGKTTLSDRLLEVTQTLSSREMRAQTLDAMDLEREKGITIKMH
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PVRLEYTAKDGQQYALHMIDCPGHVDFSYEVSRSLQACEGALLIVDASQGIEAQTLANVY
EEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHEEE
LALENNLEIIPILNKIDLPGAEVERVSEEVEHVLGLPREEIILASGKEGTGVPEILEAIV
EEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHHHHHH
ARIPPPKGINEAPARALIFDSQYNAYKGVIAYIRVVDGTIRAGDKLRLMGTEVLTEAMEI
HHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHH
GFFRPGMVAGQALSAGEVGYIATGLKSVRDCQVGDTITLVDNPAKTSLAPYEPAKPMVFA
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEE
GLYPVDSGDYTLLRDALDKLRLNDAALSFEPETSAALGFGFRAGFLGLLHMEIVQERLER
EEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
EYNIDLLVTAPSVEYQVLTNAGEILIIDNPSELPDVGQISQIEEPMMSISIIVPTRYIGV
HCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHCCHHEEEEEECHHHHHH
VMELVTGKRGIFQSMDYLDKERVVLKYEIPLSEIVIDFYDSLKSRTQGYASLDYHLSSYR
HHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
ASDLVKLDVLVNGTAVDALSMITHRDNAYRQGRQLVEKLQKLIPRQMFEVPIQAAVGSRV
CCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHH
IARETIRAMRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKMVGNVEIPQDAFMAVLKLDS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA