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The map label for this gene is speE2 [H]

Identifier: 159899376

GI number: 159899376

Start: 3627696

End: 3630149

Strand: Direct

Name: speE2 [H]

Synonym: Haur_2857

Alternate gene names: 159899376

Gene position: 3627696-3630149 (Clockwise)

Preceding gene: 159899373

Following gene: 159899377

Centisome position: 57.16

GC content: 51.96

Gene sequence:

>2454_bases
ATGACCATTGTCCTTGATCAGCGCTCCGATCAATCACTTGCCGTCTACATCGACGGCGATTTGCAGTTTGATAGCCGTGA
TGAAGGGGTGTACCACGAGCTTTTGGCCTTGCCAGCGCTCGCGCTTGCCCAAGCACGCCGCCCCAAACGCCAACTCCGAG
CGTTGATTTGCGGCGGTGGCGATGGCTTCGTTGCCCGTGAATTGCTCAAATCCTCAGCCATCAAGCAGATCGATTTGGTC
GATTATGACCCAACCATCCTCGATTTGGCCCAAAACGAATGGCCTGAACTCAATCAGCATGCCTTAACCAACCCACGCCT
AAAATTGCATATCTGCGATGCTGTGCAATTTGTCGATCTGGCGGCGCAGGCAGCCGATGAATACGATTTGATTATTTGTG
ATTTTACAGTGCCACGTGATCAAGCTGGCGCTCGTTTACACAGCATCGACTGGTATCAGCAATTGGCCTATATTTTGCAT
CCCCAAGGCTTGATTGCGGTCAATGCTGCTTCGCCATCGCAAACGCCAGTCGCTTATTGGATGATGTATAACAGCATTCG
CACTGCCGATTTGAACCCAAAGCCTTATCGTTTTGCCTTGCCTTCGTTTACCGAAGCAGGCTACGGCGATGATTGGGGCT
TTTTCATTGGTAGTCGTCGCGCGTTACGCCCACGTGAGTTTGATCATTTGGCCTTGGCTGAGCCACGCGAATTTTTGCAC
GATATCGAGCAACTGAAACGCGCCTTTGCCTTTCCCGCCGCGCTTGCCGAAAAACGCACCACCGCCCAACCAACCAAACT
AGCGAGCGATGCGCTGTTGCACAGTTTATTCGCGCCAACCTGGGATGAAGCCAGCGGCACCGATTGGACATCACTCGATT
TTGTGCATGATCCAGCGCCGTTGCCCAAACCGCATACTAGCCCAACCCTAGTGCCAAGTGCTGTCCAGCAGGCATTGCAA
GCCGATCAAGGGCTTGATGAAAGCCAAGTTTTCGAGCAAGTGGTGCAAGTGATGCCCGCTTTACGAGCCGAACAAACCCG
CAGCATGGTACGTGAATTTATCGCCAACCCCTTGCGATTTCTCAATTCGCTGGATTTGCGCAGCTTGGTTGAGGCCTTGT
TGCGGCGTAGTCGCGAATTACCACGCAAACTTGTGACCGAATTACGCTTGCTGCGTCGCCATCTGCAACGCACGTGGGAG
AATCCCCAATTGTTGCTCAGCCGTGGCCTACGAATCGTCTCGGTAATTGCCTTGGTGATTATTTTGGCCAACTTGCTACA
CCCCGACACAACCTATGCCAAAGGCGATACCTCACCAGGCTCTGGCGCAACGTCAAGTCAATCGATGGTGCGTACCAATT
CGCCCTATGATGTGGTGCAAGCGCCGCCTTCCACGGTGGCTGGGCGCGGTTTCGCAAGCTCGACCTACGGGCGCGGACCG
GTCGATGAATATGGAACCTATATTCCAACCACTCGGCGCTATCGCTACTATGGCTCATATTATGGCGGTAGCTCCTATCG
CAACTATAACCGTAACCAACGCCCAAGCGAAGATCCTAACGAGGGCGGTGCACTCTATCGGCTGACTCCCGAAACTGATA
TTTTGGCCAATGGCGAAATCGTCATTATCTTGACCGATCAAGCCTATTTGTTGCTTGCTGACCAAGTAATGACCGTGGTT
GATGCCGAAGATGGCTTGCCGATTGTCTTTTTGCAGCGCGACGAAGCCTTGATTTGGCGTACTCATCGTGAGCTTGACCG
CCAACGACGCGGGCTTGATGGCAGTGTCAAAGCGAAACAAGATTGGATCGATTGGGTCAGTTGGGCCGGCTTTGTGCCTT
GGCGCAACGATGATCAAGCCGAATTGAACAACATTGTGCAAACGCGGGGCACACTCGATCAAGCAATCAAAAGCTTAGGT
TCGATTCCGGCCAATCAGCCCGCCATTCGGGTTGCGCCAACTGCTGGGGGATTTCAATTATTCTCCAGTGTCTGGATGAA
TGCTGAAGGCAGTTTGGCGATTCTCGAACAGCCAGATGGTAGCAAGGCCTATCTCAATAAAACTGGCTGGTTCCGTGATG
CGGCACTAACCCAACCGCTTAGCGAGCCATACCCTGATGAGTTTCGCACGAAAGTTCTAGTGCCATTCTTGACCAAGCAG
ACCACGGAGTATGAGGCAATCAAAAAGCGCATCGAGGCTGATTTGACCAGTGCTAAGAGCGACTTAAGTTTGTTGAATTC
AGACAAAACCGAGTACACGTCAATTCAAACGAGCTATGGCCCAAGCGATATTGTTGATTATGGCACGACCGAAATCCGCG
TAGATGAAGCCTTGCGCCGTACCAACGACGATATTGCCCGAACCCAACAGCTCATCCAAATCTTGAATGATCAACTAAGT
TCGTTGCCCAAGCAAAACGACGCGGCCAAGAAGTTTTTACAGAGCTTGAAATAA

Upstream 100 bases:

>100_bases
CCAAAAGCCTCTAGCCATTGACATGCTGGCTATAAATCTGTAACCTATAACCAGCAACCCACGTACCAACCCCAGCAAAC
CCGTTGTATGGAGAACAACC

Downstream 100 bases:

>100_bases
TGTACGTCGCGATTCTGCCACAACTGAGCGTCAGCAGTGAACTTGGCCAGAAGTGGGAAGCTTTAGTTCAAGCCAATCCC
GCTGGTGGCGTGATGCAAAG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 817; Mature: 816

Protein sequence:

>817_residues
MTIVLDQRSDQSLAVYIDGDLQFDSRDEGVYHELLALPALALAQARRPKRQLRALICGGGDGFVARELLKSSAIKQIDLV
DYDPTILDLAQNEWPELNQHALTNPRLKLHICDAVQFVDLAAQAADEYDLIICDFTVPRDQAGARLHSIDWYQQLAYILH
PQGLIAVNAASPSQTPVAYWMMYNSIRTADLNPKPYRFALPSFTEAGYGDDWGFFIGSRRALRPREFDHLALAEPREFLH
DIEQLKRAFAFPAALAEKRTTAQPTKLASDALLHSLFAPTWDEASGTDWTSLDFVHDPAPLPKPHTSPTLVPSAVQQALQ
ADQGLDESQVFEQVVQVMPALRAEQTRSMVREFIANPLRFLNSLDLRSLVEALLRRSRELPRKLVTELRLLRRHLQRTWE
NPQLLLSRGLRIVSVIALVIILANLLHPDTTYAKGDTSPGSGATSSQSMVRTNSPYDVVQAPPSTVAGRGFASSTYGRGP
VDEYGTYIPTTRRYRYYGSYYGGSSYRNYNRNQRPSEDPNEGGALYRLTPETDILANGEIVIILTDQAYLLLADQVMTVV
DAEDGLPIVFLQRDEALIWRTHRELDRQRRGLDGSVKAKQDWIDWVSWAGFVPWRNDDQAELNNIVQTRGTLDQAIKSLG
SIPANQPAIRVAPTAGGFQLFSSVWMNAEGSLAILEQPDGSKAYLNKTGWFRDAALTQPLSEPYPDEFRTKVLVPFLTKQ
TTEYEAIKKRIEADLTSAKSDLSLLNSDKTEYTSIQTSYGPSDIVDYGTTEIRVDEALRRTNDDIARTQQLIQILNDQLS
SLPKQNDAAKKFLQSLK

Sequences:

>Translated_817_residues
MTIVLDQRSDQSLAVYIDGDLQFDSRDEGVYHELLALPALALAQARRPKRQLRALICGGGDGFVARELLKSSAIKQIDLV
DYDPTILDLAQNEWPELNQHALTNPRLKLHICDAVQFVDLAAQAADEYDLIICDFTVPRDQAGARLHSIDWYQQLAYILH
PQGLIAVNAASPSQTPVAYWMMYNSIRTADLNPKPYRFALPSFTEAGYGDDWGFFIGSRRALRPREFDHLALAEPREFLH
DIEQLKRAFAFPAALAEKRTTAQPTKLASDALLHSLFAPTWDEASGTDWTSLDFVHDPAPLPKPHTSPTLVPSAVQQALQ
ADQGLDESQVFEQVVQVMPALRAEQTRSMVREFIANPLRFLNSLDLRSLVEALLRRSRELPRKLVTELRLLRRHLQRTWE
NPQLLLSRGLRIVSVIALVIILANLLHPDTTYAKGDTSPGSGATSSQSMVRTNSPYDVVQAPPSTVAGRGFASSTYGRGP
VDEYGTYIPTTRRYRYYGSYYGGSSYRNYNRNQRPSEDPNEGGALYRLTPETDILANGEIVIILTDQAYLLLADQVMTVV
DAEDGLPIVFLQRDEALIWRTHRELDRQRRGLDGSVKAKQDWIDWVSWAGFVPWRNDDQAELNNIVQTRGTLDQAIKSLG
SIPANQPAIRVAPTAGGFQLFSSVWMNAEGSLAILEQPDGSKAYLNKTGWFRDAALTQPLSEPYPDEFRTKVLVPFLTKQ
TTEYEAIKKRIEADLTSAKSDLSLLNSDKTEYTSIQTSYGPSDIVDYGTTEIRVDEALRRTNDDIARTQQLIQILNDQLS
SLPKQNDAAKKFLQSLK
>Mature_816_residues
TIVLDQRSDQSLAVYIDGDLQFDSRDEGVYHELLALPALALAQARRPKRQLRALICGGGDGFVARELLKSSAIKQIDLVD
YDPTILDLAQNEWPELNQHALTNPRLKLHICDAVQFVDLAAQAADEYDLIICDFTVPRDQAGARLHSIDWYQQLAYILHP
QGLIAVNAASPSQTPVAYWMMYNSIRTADLNPKPYRFALPSFTEAGYGDDWGFFIGSRRALRPREFDHLALAEPREFLHD
IEQLKRAFAFPAALAEKRTTAQPTKLASDALLHSLFAPTWDEASGTDWTSLDFVHDPAPLPKPHTSPTLVPSAVQQALQA
DQGLDESQVFEQVVQVMPALRAEQTRSMVREFIANPLRFLNSLDLRSLVEALLRRSRELPRKLVTELRLLRRHLQRTWEN
PQLLLSRGLRIVSVIALVIILANLLHPDTTYAKGDTSPGSGATSSQSMVRTNSPYDVVQAPPSTVAGRGFASSTYGRGPV
DEYGTYIPTTRRYRYYGSYYGGSSYRNYNRNQRPSEDPNEGGALYRLTPETDILANGEIVIILTDQAYLLLADQVMTVVD
AEDGLPIVFLQRDEALIWRTHRELDRQRRGLDGSVKAKQDWIDWVSWAGFVPWRNDDQAELNNIVQTRGTLDQAIKSLGS
IPANQPAIRVAPTAGGFQLFSSVWMNAEGSLAILEQPDGSKAYLNKTGWFRDAALTQPLSEPYPDEFRTKVLVPFLTKQT
TEYEAIKKRIEADLTSAKSDLSLLNSDKTEYTSIQTSYGPSDIVDYGTTEIRVDEALRRTNDDIARTQQLIQILNDQLSS
LPKQNDAAKKFLQSLK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786312, Length=150, Percent_Identity=32.6666666666667, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323175, Length=196, Percent_Identity=28.0612244897959, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325326, Length=155, Percent_Identity=32.258064516129, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 91631; Mature: 91500

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVLDQRSDQSLAVYIDGDLQFDSRDEGVYHELLALPALALAQARRPKRQLRALICGGG
CEEEEECCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCC
DGFVARELLKSSAIKQIDLVDYDPTILDLAQNEWPELNQHALTNPRLKLHICDAVQFVDL
CHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCCCCCHHCCCCCCEEEEEHHHHHHHHH
AAQAADEYDLIICDFTVPRDQAGARLHSIDWYQQLAYILHPQGLIAVNAASPSQTPVAYW
HHHCCCCCCEEEEEEECCCHHHCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
MMYNSIRTADLNPKPYRFALPSFTEAGYGDDWGFFIGSRRALRPREFDHLALAEPREFLH
EEHHCEEECCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCHHHHHH
DIEQLKRAFAFPAALAEKRTTAQPTKLASDALLHSLFAPTWDEASGTDWTSLDFVHDPAP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCC
LPKPHTSPTLVPSAVQQALQADQGLDESQVFEQVVQVMPALRAEQTRSMVREFIANPLRF
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNSLDLRSLVEALLRRSRELPRKLVTELRLLRRHLQRTWENPQLLLSRGLRIVSVIALVI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHH
ILANLLHPDTTYAKGDTSPGSGATSSQSMVRTNSPYDVVQAPPSTVAGRGFASSTYGRGP
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCC
VDEYGTYIPTTRRYRYYGSYYGGSSYRNYNRNQRPSEDPNEGGALYRLTPETDILANGEI
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCE
VIILTDQAYLLLADQVMTVVDAEDGLPIVFLQRDEALIWRTHRELDRQRRGLDGSVKAKQ
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
DWIDWVSWAGFVPWRNDDQAELNNIVQTRGTLDQAIKSLGSIPANQPAIRVAPTAGGFQL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHH
FSSVWMNAEGSLAILEQPDGSKAYLNKTGWFRDAALTQPLSEPYPDEFRTKVLVPFLTKQ
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHEEHHHHHHCC
TTEYEAIKKRIEADLTSAKSDLSLLNSDKTEYTSIQTSYGPSDIVDYGTTEIRVDEALRR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCEEEHHHHHHH
TNDDIARTQQLIQILNDQLSSLPKQNDAAKKFLQSLK
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TIVLDQRSDQSLAVYIDGDLQFDSRDEGVYHELLALPALALAQARRPKRQLRALICGGG
EEEEECCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCC
DGFVARELLKSSAIKQIDLVDYDPTILDLAQNEWPELNQHALTNPRLKLHICDAVQFVDL
CHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCCCCCHHCCCCCCEEEEEHHHHHHHHH
AAQAADEYDLIICDFTVPRDQAGARLHSIDWYQQLAYILHPQGLIAVNAASPSQTPVAYW
HHHCCCCCCEEEEEEECCCHHHCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
MMYNSIRTADLNPKPYRFALPSFTEAGYGDDWGFFIGSRRALRPREFDHLALAEPREFLH
EEHHCEEECCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCHHHHHH
DIEQLKRAFAFPAALAEKRTTAQPTKLASDALLHSLFAPTWDEASGTDWTSLDFVHDPAP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCC
LPKPHTSPTLVPSAVQQALQADQGLDESQVFEQVVQVMPALRAEQTRSMVREFIANPLRF
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNSLDLRSLVEALLRRSRELPRKLVTELRLLRRHLQRTWENPQLLLSRGLRIVSVIALVI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHH
ILANLLHPDTTYAKGDTSPGSGATSSQSMVRTNSPYDVVQAPPSTVAGRGFASSTYGRGP
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCC
VDEYGTYIPTTRRYRYYGSYYGGSSYRNYNRNQRPSEDPNEGGALYRLTPETDILANGEI
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCE
VIILTDQAYLLLADQVMTVVDAEDGLPIVFLQRDEALIWRTHRELDRQRRGLDGSVKAKQ
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
DWIDWVSWAGFVPWRNDDQAELNNIVQTRGTLDQAIKSLGSIPANQPAIRVAPTAGGFQL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHH
FSSVWMNAEGSLAILEQPDGSKAYLNKTGWFRDAALTQPLSEPYPDEFRTKVLVPFLTKQ
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHEEHHHHHHCC
TTEYEAIKKRIEADLTSAKSDLSLLNSDKTEYTSIQTSYGPSDIVDYGTTEIRVDEALRR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHCCCCEEEHHHHHHH
TNDDIARTQQLIQILNDQLSSLPKQNDAAKKFLQSLK
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]