Click here to switch to the map view.

The map label for this gene is punA [H]

Identifier: 159899229

GI number: 159899229

Start: 3464000

End: 3464845

Strand: Direct

Name: punA [H]

Synonym: Haur_2710

Alternate gene names: 159899229

Gene position: 3464000-3464845 (Clockwise)

Preceding gene: 159899228

Following gene: 159899230

Centisome position: 54.58

GC content: 55.32

Gene sequence:

>846_bases
ATGACCACCAACCGAACTCAAGCAATCACTGGCGCAGTCGCTGCCATTCGTCAATATACCGAGCGCGTACCCCAAGTTGG
CATTATCCTTGGCTCTGGCTTGAGTCAACTTGTTGATCATATTCAGGATGCCGTGGTAGTTCCCTACACTGCGATTCCAG
GCTTCGCACCTTCAGCGGTGCCAGGCCATCGCGGTGAACTTGTTTTAGGTGAACTTGGTGGGGTTTCTGTGCTGGCAATG
CGTGGCCGCTTTCACTTCTACGAAGGCTATGCCATGGACGAAGTAACCTTGCCTGTGCATGTGATGCGAGCGCTGGGAGC
CGAGATATTAATTGTTACGAATGCGGCTGGTGGCTTAAATGCCAATTGGCAGGTCGGCGACTTAATGCGCATCAGCGACC
ATATTTTTATGCCAGGCATGGCGGGCTTTCACCCATTGCGCGGCCACAACGACGATACGCTTGGCCCACGCTTCCCAGCC
ATGCTCAACGCCTATGATTCTGAATTAGGTGCAATGGCCAAGGCTGCCGCTGAACGGGCTGGCGCAACTCTGCGCGAAGG
GGTTTATGCCATGCTAGCCGGGCCATCGTTTGAAACTGGCGCTGAGATGAACTATCTGCGCGGGGTTGGGGTTGACGCTG
TGGGCATGTCTACCGCCCCTGAAACGATTGTGGCTCGCTACCGTGGTATGCGCGTCTTGGGCATTTCGTTGATCACCAAT
ATTGCCCACCCCGACGCGCCACCAGCCAACCATGAAGAGGTGCTTGAGGCAGGCGAAACTGCCAAGCCCATGTTCAGCGC
ATTAATCACCGATGTGCTAAGCCAAATCGCTGCCGATGGCAAGTAG

Upstream 100 bases:

>100_bases
TACTGATGCTGATTTTGAGGCCAAAAAACGCGAAATTTTAAGTCGGATATAATGCCTGCGGCCATAGCAATGTGGCTATT
GTTGCAAAGGATTTCAATTC

Downstream 100 bases:

>100_bases
TTTGCGTGGCGTAGGCTTATTGCTGATAGGAATTGTAATTGGCATGATCATTGGGGTGTGGTGGTTAGCGCCACGCCTCA
ATGGAACGATTATTCGCTTT

Product: inosine guanosine and xanthosine phosphorylase family protein

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTTNRTQAITGAVAAIRQYTERVPQVGIILGSGLSQLVDHIQDAVVVPYTAIPGFAPSAVPGHRGELVLGELGGVSVLAM
RGRFHFYEGYAMDEVTLPVHVMRALGAEILIVTNAAGGLNANWQVGDLMRISDHIFMPGMAGFHPLRGHNDDTLGPRFPA
MLNAYDSELGAMAKAAAERAGATLREGVYAMLAGPSFETGAEMNYLRGVGVDAVGMSTAPETIVARYRGMRVLGISLITN
IAHPDAPPANHEEVLEAGETAKPMFSALITDVLSQIAADGK

Sequences:

>Translated_281_residues
MTTNRTQAITGAVAAIRQYTERVPQVGIILGSGLSQLVDHIQDAVVVPYTAIPGFAPSAVPGHRGELVLGELGGVSVLAM
RGRFHFYEGYAMDEVTLPVHVMRALGAEILIVTNAAGGLNANWQVGDLMRISDHIFMPGMAGFHPLRGHNDDTLGPRFPA
MLNAYDSELGAMAKAAAERAGATLREGVYAMLAGPSFETGAEMNYLRGVGVDAVGMSTAPETIVARYRGMRVLGISLITN
IAHPDAPPANHEEVLEAGETAKPMFSALITDVLSQIAADGK
>Mature_280_residues
TTNRTQAITGAVAAIRQYTERVPQVGIILGSGLSQLVDHIQDAVVVPYTAIPGFAPSAVPGHRGELVLGELGGVSVLAMR
GRFHFYEGYAMDEVTLPVHVMRALGAEILIVTNAAGGLNANWQVGDLMRISDHIFMPGMAGFHPLRGHNDDTLGPRFPAM
LNAYDSELGAMAKAAAERAGATLREGVYAMLAGPSFETGAEMNYLRGVGVDAVGMSTAPETIVARYRGMRVLGISLITNI
AHPDAPPANHEEVLEAGETAKPMFSALITDVLSQIAADGK

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=263, Percent_Identity=49.8098859315589, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1788746, Length=269, Percent_Identity=43.1226765799257, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17541190, Length=271, Percent_Identity=47.9704797047971, Blast_Score=239, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6323238, Length=304, Percent_Identity=43.75, Blast_Score=214, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=45.7692307692308, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24656090, Length=260, Percent_Identity=45.7692307692308, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=45.7692307692308, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=45.7692307692308, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24762376, Length=221, Percent_Identity=37.10407239819, Blast_Score=137, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29645; Mature: 29514

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNRTQAITGAVAAIRQYTERVPQVGIILGSGLSQLVDHIQDAVVVPYTAIPGFAPSAV
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEECCCCCCCCCCCCC
PGHRGELVLGELGGVSVLAMRGRFHFYEGYAMDEVTLPVHVMRALGAEILIVTNAAGGLN
CCCCCCEEEECCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
ANWQVGDLMRISDHIFMPGMAGFHPLRGHNDDTLGPRFPAMLNAYDSELGAMAKAAAERA
CCCCCCHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATLREGVYAMLAGPSFETGAEMNYLRGVGVDAVGMSTAPETIVARYRGMRVLGISLITN
CCHHHHCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHH
IAHPDAPPANHEEVLEAGETAKPMFSALITDVLSQIAADGK
HCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTNRTQAITGAVAAIRQYTERVPQVGIILGSGLSQLVDHIQDAVVVPYTAIPGFAPSAV
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCEEECCCCCCCCCCCCC
PGHRGELVLGELGGVSVLAMRGRFHFYEGYAMDEVTLPVHVMRALGAEILIVTNAAGGLN
CCCCCCEEEECCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
ANWQVGDLMRISDHIFMPGMAGFHPLRGHNDDTLGPRFPAMLNAYDSELGAMAKAAAERA
CCCCCCHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATLREGVYAMLAGPSFETGAEMNYLRGVGVDAVGMSTAPETIVARYRGMRVLGISLITN
CCHHHHCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHH
IAHPDAPPANHEEVLEAGETAKPMFSALITDVLSQIAADGK
HCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]