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The map label for this gene is potD [H]

Identifier: 159897593

GI number: 159897593

Start: 1216770

End: 1217891

Strand: Direct

Name: potD [H]

Synonym: Haur_1064

Alternate gene names: 159897593

Gene position: 1216770-1217891 (Clockwise)

Preceding gene: 159897592

Following gene: 159897598

Centisome position: 19.17

GC content: 49.29

Gene sequence:

>1122_bases
ATGAAACTACGTCGCTTTGGGCTGGTGCTCATGGTGTTGAGTTTGTTGGTGGCCTGTGGCGAAGCCAGCTCAACCGCTGT
TCCGACAACTGCGCCTGCTGGTACGGCCACTGGCTCGAATGCTGGCTCGGTTGATCGCAGCAAATTGAGCAAAACCTTGC
GAATTTATGCGTGGGGCGAGTATGTTCCCGAAGATGTGCCACAACTGTTTGAGAGTGAGTTTGGGGTCAAGGTTACGGTT
GATACCTATTCATCGAACGAAGAAATGGCCGCCAAAATTCGCGCTGGCAATTCAGGCTACGATTTGATTCAACCTTCAGA
TTATATGGTGGCGTTGTTGGCCGAGGGCAATTATTTGGCCAAAATTGATTTGGCTAATATTCCCAACATTGCCAATATCG
ACCCAGCCAATATGGGTTTGTATTACGACCCAAACAATGAATTTTCAGTACCATACCTTTGGGGAACCACGGGGATTGCC
TACGATAAAACCGCAGTTTCACCAGCCCCAACTAGCTGGTCGATTTTGTTTGATCCAGCGCAATTGAGCGCCTACAAAGG
TCGCGTGAGCATGCTCAACGACGAGCGCGAGGTAATTGGCGCGGCTATGCTGTTCTTGGGCAAAGATCGCAATTCCAGCG
ATGCGGCGGATCTCGAAGCCGCCAAAAAGGTTTTGATTGAACAAAAGCCATTGCTAGCCAAATATAACAGCGATAATGTT
TATCAAGATTTGGCTTCGGGCGAAGTGGTTTTGGCCCAATCGTGGAATAATTACACGGGTTTGGCCATGATCGATAATGA
AAACATCGAGTGGGTGATTCCGCAAGAGGGTGGCGTGATTTGGCAAGATACCATGGCGATTGTGGCTGGCACGCCCAACC
AATATACTGCCGAAGTATTCATTGATTTTATGAATCGGCCAGAAATTGCCGCCAAAGTTGCCGACTTTACTGGTGCTTTG
ACTCCCAACGTCAAGGGCGAACCATTGATCGGCGACGATCTCAAGGCTGTCTATCCCAAGATCAAACCGAGCGCTGAAGA
TCGCAAACGGCTTGATTGGTTGCGTAAAGGCCAAAATGCGACGGCCTTCTCCGATGTGTGGTCGGCGGTTAAATCGCAAT
AA

Upstream 100 bases:

>100_bases
TGTTACTTGCTTCAACGACCTTGGTTGTGACTTCGTTGTGGATGCAACGCCGTCGCCAAGGCTAATTATTGCTATTGGTG
TGTTTTAAGGAGTTTGATTG

Downstream 100 bases:

>100_bases
CGCAGCACAGAGAACAAGGTGGGTTCATAAGCTTGACGGTGGATTCCGTTCCCTCACCTACTCATTTAATATAGGATTGA
GCCTATGATTTAGCCAGAGG

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKLRRFGLVLMVLSLLVACGEASSTAVPTTAPAGTATGSNAGSVDRSKLSKTLRIYAWGEYVPEDVPQLFESEFGVKVTV
DTYSSNEEMAAKIRAGNSGYDLIQPSDYMVALLAEGNYLAKIDLANIPNIANIDPANMGLYYDPNNEFSVPYLWGTTGIA
YDKTAVSPAPTSWSILFDPAQLSAYKGRVSMLNDEREVIGAAMLFLGKDRNSSDAADLEAAKKVLIEQKPLLAKYNSDNV
YQDLASGEVVLAQSWNNYTGLAMIDNENIEWVIPQEGGVIWQDTMAIVAGTPNQYTAEVFIDFMNRPEIAAKVADFTGAL
TPNVKGEPLIGDDLKAVYPKIKPSAEDRKRLDWLRKGQNATAFSDVWSAVKSQ

Sequences:

>Translated_373_residues
MKLRRFGLVLMVLSLLVACGEASSTAVPTTAPAGTATGSNAGSVDRSKLSKTLRIYAWGEYVPEDVPQLFESEFGVKVTV
DTYSSNEEMAAKIRAGNSGYDLIQPSDYMVALLAEGNYLAKIDLANIPNIANIDPANMGLYYDPNNEFSVPYLWGTTGIA
YDKTAVSPAPTSWSILFDPAQLSAYKGRVSMLNDEREVIGAAMLFLGKDRNSSDAADLEAAKKVLIEQKPLLAKYNSDNV
YQDLASGEVVLAQSWNNYTGLAMIDNENIEWVIPQEGGVIWQDTMAIVAGTPNQYTAEVFIDFMNRPEIAAKVADFTGAL
TPNVKGEPLIGDDLKAVYPKIKPSAEDRKRLDWLRKGQNATAFSDVWSAVKSQ
>Mature_373_residues
MKLRRFGLVLMVLSLLVACGEASSTAVPTTAPAGTATGSNAGSVDRSKLSKTLRIYAWGEYVPEDVPQLFESEFGVKVTV
DTYSSNEEMAAKIRAGNSGYDLIQPSDYMVALLAEGNYLAKIDLANIPNIANIDPANMGLYYDPNNEFSVPYLWGTTGIA
YDKTAVSPAPTSWSILFDPAQLSAYKGRVSMLNDEREVIGAAMLFLGKDRNSSDAADLEAAKKVLIEQKPLLAKYNSDNV
YQDLASGEVVLAQSWNNYTGLAMIDNENIEWVIPQEGGVIWQDTMAIVAGTPNQYTAEVFIDFMNRPEIAAKVADFTGAL
TPNVKGEPLIGDDLKAVYPKIKPSAEDRKRLDWLRKGQNATAFSDVWSAVKSQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=307, Percent_Identity=37.785016286645, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1787078, Length=322, Percent_Identity=31.6770186335404, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787711, Length=305, Percent_Identity=26.5573770491803, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40621; Mature: 40621

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRRFGLVLMVLSLLVACGEASSTAVPTTAPAGTATGSNAGSVDRSKLSKTLRIYAWGE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCC
YVPEDVPQLFESEFGVKVTVDTYSSNEEMAAKIRAGNSGYDLIQPSDYMVALLAEGNYLA
CCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEE
KIDLANIPNIANIDPANMGLYYDPNNEFSVPYLWGTTGIAYDKTAVSPAPTSWSILFDPA
EEECCCCCCCCCCCCCCCCEEECCCCCEECEEEECCCCCEECCCCCCCCCCCEEEEECHH
QLSAYKGRVSMLNDEREVIGAAMLFLGKDRNSSDAADLEAAKKVLIEQKPLLAKYNSDNV
HHHHHCCCCEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHH
YQDLASGEVVLAQSWNNYTGLAMIDNENIEWVIPQEGGVIWQDTMAIVAGTPNQYTAEVF
HHHHCCCCEEEEECCCCEEEEEEEECCCEEEEEECCCCEEEECCEEEEECCCCCEEEEHH
IDFMNRPEIAAKVADFTGALTPNVKGEPLIGDDLKAVYPKIKPSAEDRKRLDWLRKGQNA
EECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCC
TAFSDVWSAVKSQ
CHHHHHHHHHHCC
>Mature Secondary Structure
MKLRRFGLVLMVLSLLVACGEASSTAVPTTAPAGTATGSNAGSVDRSKLSKTLRIYAWGE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCC
YVPEDVPQLFESEFGVKVTVDTYSSNEEMAAKIRAGNSGYDLIQPSDYMVALLAEGNYLA
CCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEE
KIDLANIPNIANIDPANMGLYYDPNNEFSVPYLWGTTGIAYDKTAVSPAPTSWSILFDPA
EEECCCCCCCCCCCCCCCCEEECCCCCEECEEEECCCCCEECCCCCCCCCCCEEEEECHH
QLSAYKGRVSMLNDEREVIGAAMLFLGKDRNSSDAADLEAAKKVLIEQKPLLAKYNSDNV
HHHHHCCCCEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHH
YQDLASGEVVLAQSWNNYTGLAMIDNENIEWVIPQEGGVIWQDTMAIVAGTPNQYTAEVF
HHHHCCCCEEEEECCCCEEEEEEEECCCEEEEEECCCCEEEECCEEEEECCCCCEEEEHH
IDFMNRPEIAAKVADFTGALTPNVKGEPLIGDDLKAVYPKIKPSAEDRKRLDWLRKGQNA
EECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCC
TAFSDVWSAVKSQ
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]