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The map label for this gene is glpK [H]

Identifier: 159897327

GI number: 159897327

Start: 901716

End: 903206

Strand: Reverse

Name: glpK [H]

Synonym: Haur_0798

Alternate gene names: 159897327

Gene position: 903206-901716 (Counterclockwise)

Preceding gene: 159897329

Following gene: 159897326

Centisome position: 14.23

GC content: 54.19

Gene sequence:

>1491_bases
ATGCAGTATATTGCGGCACTCGACCAAGGTACAACCAGCACGCGCTGTATGGTTTTCGACCATAATGGCAATGTTGTCGG
CGTTGATCAACGTGAGCATACCCAGATTTTCCCGCAGCCAGGCTGGGTCGAGCATAATCCCGCCGAGATTTGGCAACGAA
CCCAGGGCGTGATCGCAGGAGCACTAGCGGCTGCCAACATCGAAAAAAGCGATCTGGCGGCGCTGGGCATTACCAATCAG
CGTGAAACAACCGTCATTTGGGATCGCCACACCGGCAAGGCAATCTACAATGCTTTAGTTTGGCAAGATACCCGCACTGA
TGCGCTGGTGCGCCAATTTGCTGCTGACGGTGGACAAGATCGTTTTCGGGCGCAAACTGGATTGCCCTTGGCGACCTATT
TTTCAGCCACCAAAATTCGTTGGCTGCTTGATCAGGTTGAGGGTGCGCAAGCAGCGGCTGAAGCAGGCGATTTGTTGTTT
GGCACGATCGATTGTTTTGTTGCTTGGTGGTTGACGGGTGGCCCCCATGGTGGATTGCACATCACCGACACAACGAATGC
CTCACGTACCTTGCTGCTCAACCTCACGACTGGCGATTGGGATGCTGAATTATTGGAGATTTTTCGCATTCCGCGCAGCA
TGTTGCCAAATGTGCGCTCCTCAAGCGAGGTGTATGGGACATGTCGCGGCCAACTCGATGGCGTGCCATTAGCAGGAATT
TTGGGTGATCAACAAGCTGCAACGGTTGGGCAAGTATGCTTTGAAGTTGGCGAGGCCAAAAATACCTATGGCACTGGCAA
TTTTATGCTGCTCAATACTGGTGAGCGCTTGGTGCAATCGCGCCATGGCTTAATTACCACGCCAGCCTATCATTTTGGCA
AGCAGCCAACGTGGGCCTTAGAAGGCTCAATCGCAGTAACTGGCTCGTTGGTGCAATGGCTACGCGACAATTTGGGGATT
ATCCACAGTGCGGCTGAGGTCGAACCGTTGGCTGCCAGCGTAGCCGATAACGGTGGCGTATATCTGGTGCCAGCCTTTTC
GGGCTTGTTTGCCCCGCATTGGCGCTCCGATGCGCGAGGCGTGTTGGTTGGGCTAACGCGCTATGCCAATAAAGCTCATA
TCGCGCGAGCGGCGCTTGAAGCCACTGCCTACCAAACCCGCGAAGTCGTCAGCGCCATGGCCCAAGATTCAGGGGTTGAG
TTGACGGCGCTCAAAGTTGATGGCGGAATGGTGGCGAATGAATTGCTTATGCAGTTTCAGGCTGATATTTTGGGTGTACC
AGTTGTGCGGCCCAAAGTTGCCGAAACCACCGCCTTGGGGGCTGCCTACGCCGCTGGTTTGGCTGTTGGCTACTGGGCGA
ATCCAGCTGAATTACGCGCCAACTGGAGCGAAAGCAAGCGTTGGGAGCCGCAAATGAGCAGCGATCAGCGTGATAGCTTG
TATGCACAATGGAACAAAGCAGTGCAACGCACGCTCAATTGGCAAGAATAA

Upstream 100 bases:

>100_bases
ATAGCCTACTACCTTCGTGATCTTCGTGTCCTTCGTGGATCAAAATCCAGATCCCTTATCCCTGACCCCTAACCCACACA
TTACAATGAGGTAAGCTTGT

Downstream 100 bases:

>100_bases
GCTGGCAAATCCGTCTTGATACTAGCCAAATCAGCGGTTGGTTTGGCTACCAAATTGCTACTTTAGAAAACCTACAATCG
GTGGTATTGTGGGTATATCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MQYIAALDQGTTSTRCMVFDHNGNVVGVDQREHTQIFPQPGWVEHNPAEIWQRTQGVIAGALAAANIEKSDLAALGITNQ
RETTVIWDRHTGKAIYNALVWQDTRTDALVRQFAADGGQDRFRAQTGLPLATYFSATKIRWLLDQVEGAQAAAEAGDLLF
GTIDCFVAWWLTGGPHGGLHITDTTNASRTLLLNLTTGDWDAELLEIFRIPRSMLPNVRSSSEVYGTCRGQLDGVPLAGI
LGDQQAATVGQVCFEVGEAKNTYGTGNFMLLNTGERLVQSRHGLITTPAYHFGKQPTWALEGSIAVTGSLVQWLRDNLGI
IHSAAEVEPLAASVADNGGVYLVPAFSGLFAPHWRSDARGVLVGLTRYANKAHIARAALEATAYQTREVVSAMAQDSGVE
LTALKVDGGMVANELLMQFQADILGVPVVRPKVAETTALGAAYAAGLAVGYWANPAELRANWSESKRWEPQMSSDQRDSL
YAQWNKAVQRTLNWQE

Sequences:

>Translated_496_residues
MQYIAALDQGTTSTRCMVFDHNGNVVGVDQREHTQIFPQPGWVEHNPAEIWQRTQGVIAGALAAANIEKSDLAALGITNQ
RETTVIWDRHTGKAIYNALVWQDTRTDALVRQFAADGGQDRFRAQTGLPLATYFSATKIRWLLDQVEGAQAAAEAGDLLF
GTIDCFVAWWLTGGPHGGLHITDTTNASRTLLLNLTTGDWDAELLEIFRIPRSMLPNVRSSSEVYGTCRGQLDGVPLAGI
LGDQQAATVGQVCFEVGEAKNTYGTGNFMLLNTGERLVQSRHGLITTPAYHFGKQPTWALEGSIAVTGSLVQWLRDNLGI
IHSAAEVEPLAASVADNGGVYLVPAFSGLFAPHWRSDARGVLVGLTRYANKAHIARAALEATAYQTREVVSAMAQDSGVE
LTALKVDGGMVANELLMQFQADILGVPVVRPKVAETTALGAAYAAGLAVGYWANPAELRANWSESKRWEPQMSSDQRDSL
YAQWNKAVQRTLNWQE
>Mature_496_residues
MQYIAALDQGTTSTRCMVFDHNGNVVGVDQREHTQIFPQPGWVEHNPAEIWQRTQGVIAGALAAANIEKSDLAALGITNQ
RETTVIWDRHTGKAIYNALVWQDTRTDALVRQFAADGGQDRFRAQTGLPLATYFSATKIRWLLDQVEGAQAAAEAGDLLF
GTIDCFVAWWLTGGPHGGLHITDTTNASRTLLLNLTTGDWDAELLEIFRIPRSMLPNVRSSSEVYGTCRGQLDGVPLAGI
LGDQQAATVGQVCFEVGEAKNTYGTGNFMLLNTGERLVQSRHGLITTPAYHFGKQPTWALEGSIAVTGSLVQWLRDNLGI
IHSAAEVEPLAASVADNGGVYLVPAFSGLFAPHWRSDARGVLVGLTRYANKAHIARAALEATAYQTREVVSAMAQDSGVE
LTALKVDGGMVANELLMQFQADILGVPVVRPKVAETTALGAAYAAGLAVGYWANPAELRANWSESKRWEPQMSSDQRDSL
YAQWNKAVQRTLNWQE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=50.2982107355865, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=50.2982107355865, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=49.705304518664, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=49.9005964214712, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=31.7647058823529, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=52.6104417670683, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1790005, Length=488, Percent_Identity=25.8196721311475, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787789, Length=526, Percent_Identity=25.4752851711027, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789987, Length=458, Percent_Identity=24.0174672489083, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=44.4664031620553, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71986140, Length=531, Percent_Identity=23.728813559322, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71986147, Length=263, Percent_Identity=28.1368821292776, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321755, Length=507, Percent_Identity=40.0394477317554, Blast_Score=332, Evalue=1e-91,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=43.7007874015748, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24655555, Length=504, Percent_Identity=42.0634920634921, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655560, Length=504, Percent_Identity=42.0634920634921, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655570, Length=504, Percent_Identity=42.0634920634921, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655574, Length=504, Percent_Identity=42.0634920634921, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655565, Length=504, Percent_Identity=42.0634920634921, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=45.1685393258427, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=35.361216730038, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=35.9756097560976, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=29.2635658914729, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=29.1115311909263, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=30, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45552909, Length=271, Percent_Identity=28.7822878228782, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 53889; Mature: 53889

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYIAALDQGTTSTRCMVFDHNGNVVGVDQREHTQIFPQPGWVEHNPAEIWQRTQGVIAG
CCCEEECCCCCCCCEEEEEECCCCEEECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
ALAAANIEKSDLAALGITNQRETTVIWDRHTGKAIYNALVWQDTRTDALVRQFAADGGQD
HHHHCCCCHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCH
RFRAQTGLPLATYFSATKIRWLLDQVEGAQAAAEAGDLLFGTIDCFVAWWLTGGPHGGLH
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCEE
ITDTTNASRTLLLNLTTGDWDAELLEIFRIPRSMLPNVRSSSEVYGTCRGQLDGVPLAGI
EEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCEEEE
LGDQQAATVGQVCFEVGEAKNTYGTGNFMLLNTGERLVQSRHGLITTPAYHFGKQPTWAL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHCCCEEECCHHHCCCCCCEEE
EGSIAVTGSLVQWLRDNLGIIHSAAEVEPLAASVADNGGVYLVPAFSGLFAPHWRSDARG
CCCEEEHHHHHHHHHHCCCEEECCHHCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCE
VLVGLTRYANKAHIARAALEATAYQTREVVSAMAQDSGVELTALKVDGGMVANELLMQFQ
EEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHH
ADILGVPVVRPKVAETTALGAAYAAGLAVGYWANPAELRANWSESKRWEPQMSSDQRDSL
HHHCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHH
YAQWNKAVQRTLNWQE
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQYIAALDQGTTSTRCMVFDHNGNVVGVDQREHTQIFPQPGWVEHNPAEIWQRTQGVIAG
CCCEEECCCCCCCCEEEEEECCCCEEECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
ALAAANIEKSDLAALGITNQRETTVIWDRHTGKAIYNALVWQDTRTDALVRQFAADGGQD
HHHHCCCCHHHEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCH
RFRAQTGLPLATYFSATKIRWLLDQVEGAQAAAEAGDLLFGTIDCFVAWWLTGGPHGGLH
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCEE
ITDTTNASRTLLLNLTTGDWDAELLEIFRIPRSMLPNVRSSSEVYGTCRGQLDGVPLAGI
EEECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCEEEE
LGDQQAATVGQVCFEVGEAKNTYGTGNFMLLNTGERLVQSRHGLITTPAYHFGKQPTWAL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHCCCEEECCHHHCCCCCCEEE
EGSIAVTGSLVQWLRDNLGIIHSAAEVEPLAASVADNGGVYLVPAFSGLFAPHWRSDARG
CCCEEEHHHHHHHHHHCCCEEECCHHCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCE
VLVGLTRYANKAHIARAALEATAYQTREVVSAMAQDSGVELTALKVDGGMVANELLMQFQ
EEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHH
ADILGVPVVRPKVAETTALGAAYAAGLAVGYWANPAELRANWSESKRWEPQMSSDQRDSL
HHHCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHH
YAQWNKAVQRTLNWQE
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA