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The map label for this gene is ywlF [H]

Identifier: 159897020

GI number: 159897020

Start: 568414

End: 568863

Strand: Direct

Name: ywlF [H]

Synonym: Haur_0488

Alternate gene names: 159897020

Gene position: 568414-568863 (Clockwise)

Preceding gene: 159897019

Following gene: 159897024

Centisome position: 8.96

GC content: 50.44

Gene sequence:

>450_bases
ATGATTATGAAAATTGCGGTCGGAACCGATCATGGTGGTTTGATTTTGAAGCAAGCAGTAATTGAAGAAGTGCAACGGCT
GGGCCATGAAGTGCTCGATTTTGGCACCGATTCGCCTGCTTCGGTCGATTATCCCGATTTTGCTGCGGCTGTGGGTAATG
CTGTAGTAAGTGGCCAAGCTGAACGTGGCATTGCGATTTGCGGTAGTGGCGTTGGCGTGACGGTCGCCGCCAATAAAATT
AAAGGGGTGCGAGCCTGTTTGTGCCACGACACCTATTCGGCGCATCAAGGGGTTGAGCACGACGATCTGAATGTATTGTG
TTTGGGCGGGCGGGTGATTGGCCCAGCCTTGGCTGGCGAAATTGTCCAAGCATTTTTGAGCGCAACGTTTCACAATGAAG
CTCGCTTTACTCGGCGCTTGGATAAAGTGATTGCCTTAGAAAACCAATAA

Upstream 100 bases:

>100_bases
TCGTGCCAAGGCCTTGTTGGCCAAATAATTAATCGATCGATCAAACGGATAACGCGAAGGACGAGTTGCTCGTGCTTCGC
GTTATGCTGTGGAAAGGCAA

Downstream 100 bases:

>100_bases
ATTACGGCTAAAACAAAGCCCCGTGCTTATTACAAGCACGGGGCTTTTGAATTTAGGTGATTAGTTTAGTTCTTGGTGAT
TGCTGGCAAGAAGACCAACG

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MIMKIAVGTDHGGLILKQAVIEEVQRLGHEVLDFGTDSPASVDYPDFAAAVGNAVVSGQAERGIAICGSGVGVTVAANKI
KGVRACLCHDTYSAHQGVEHDDLNVLCLGGRVIGPALAGEIVQAFLSATFHNEARFTRRLDKVIALENQ

Sequences:

>Translated_149_residues
MIMKIAVGTDHGGLILKQAVIEEVQRLGHEVLDFGTDSPASVDYPDFAAAVGNAVVSGQAERGIAICGSGVGVTVAANKI
KGVRACLCHDTYSAHQGVEHDDLNVLCLGGRVIGPALAGEIVQAFLSATFHNEARFTRRLDKVIALENQ
>Mature_149_residues
MIMKIAVGTDHGGLILKQAVIEEVQRLGHEVLDFGTDSPASVDYPDFAAAVGNAVVSGQAERGIAICGSGVGVTVAANKI
KGVRACLCHDTYSAHQGVEHDDLNVLCLGGRVIGPALAGEIVQAFLSATFHNEARFTRRLDKVIALENQ

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=148, Percent_Identity=43.2432432432432, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15636; Mature: 15636

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKIAVGTDHGGLILKQAVIEEVQRLGHEVLDFGTDSPASVDYPDFAAAVGNAVVSGQA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCC
ERGIAICGSGVGVTVAANKIKGVRACLCHDTYSAHQGVEHDDLNVLCLGGRVIGPALAGE
CCCEEEECCCCEEEEEHHHHCHHHHHHHCCCHHHHCCCCCCCCEEEEECCEEECHHHHHH
IVQAFLSATFHNEARFTRRLDKVIALENQ
HHHHHHHHHHCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIMKIAVGTDHGGLILKQAVIEEVQRLGHEVLDFGTDSPASVDYPDFAAAVGNAVVSGQA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCC
ERGIAICGSGVGVTVAANKIKGVRACLCHDTYSAHQGVEHDDLNVLCLGGRVIGPALAGE
CCCEEEECCCCEEEEEHHHHCHHHHHHHCCCHHHHCCCCCCCCEEEEECCEEECHHHHHH
IVQAFLSATFHNEARFTRRLDKVIALENQ
HHHHHHHHHHCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]