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The map label for this gene is topB

Identifier: 15927834

GI number: 15927834

Start: 2311025

End: 2313160

Strand: Reverse

Name: topB

Synonym: SA2051

Alternate gene names: 15927834

Gene position: 2313160-2311025 (Counterclockwise)

Preceding gene: 15927838

Following gene: 15927833

Centisome position: 82.18

GC content: 33.15

Gene sequence:

>2136_bases
ATGAAATCTTTAATATTAGCTGAAAAACCATCAGTTGCAAGAGATATTGCTGATGCTTTACAAATAAATCAGAAGCGTAA
TGGTTACTTTGAAAATAACCAATATATTGTCACGTGGGCGTTAGGTCATCTAGTGACAAATGCGACACCTGAACAATACG
ATAAAAATTTAAAGGAATGGCGATTAGAAGACCTTCCAATTATACCTAAATATATGAAAACTGTTGTTATTGGTAAAACA
AGCAAACAATTTAAAACAGTAAAAGCGTTAATTTTAGATAATAAAGTGAAAGATATTATTATTGCAACAGATGCTGGACG
AGAAGGTGAACTAGTTGCAAGATTGATTTTGGATAAAGTTGGTAACAAAAAGCCAATCCGTCGATTATGGATTAGCTCAG
TTACTAAAAAAGCTATTCAACAAGGTTTTAAAAATTTAAAAGACGGTCGTCAATATAACGATTTGTATTATGCAGCGTTA
GCGAGAAGCGAGGCAGATTGGATTGTTGGGATTAATGCAACGCGTGCACTAACAACAAAGTATGATGCACAGCTATCCCT
GGGACGTGTTCAGACACCAACGATTCAATTAGTAAATACACGACAACAAGAGATTAATCAGTTCAAACCACAACAATACT
TTACATTATCATTAACGGTAAAAGGGTTTGATTTTCAGCTAGAATCAAATCAGCGATATACCAATAAAGAAACTTTAGAA
CAGATGGTTAATAATTTGAAAAATGTCGATGGTAAGATTAAATCTGTTGCTACTAAACATAAGAAGTCGTATCCGCAATC
ACTGTACAATTTAACAGATTTACAACAAGATATGTATAGACGTTATAAAATTGGACCTAAAGAAACATTGAATACACTTC
AAAGCTTATATGAGAGACATAAAGTCGTAACCTATCCAAGAACAGATTCAAACTATTTAACAACTGATATGGTAGATACT
ATGAAAGAACGTATTCAGGCGACGATGGCAACAACATATAAAGACCAAGCACGCCCATTAATGTCTAAAACATTTTCATC
AAAAATGTCGATATTTAATAATCAAAAAGTATCTGATCACCATGCAATTATTCCTACAGAAGTGAGACCTGTCATGTCAG
ACTTAAGTAATAGAGAATTAAAGTTATACGATATGATTGTCGAGCGTTTTTTAGAAGCTTTAATGCCTCCGCACGAGTAT
GACGCGATAACTGTAACTTTAGAGGTTGCAGGGCACACATTTGTTTTGAAAGAGAATGTAACAACTGTTTTAGGTTTTAA
ATCTATTAGACAAGGTGAATCTATTACAGAGATGCAACAGCCTTTTTCAGAAGGCGATGAAGTGAAGATTTCAAAAACAA
ACATTAGAGAACATGAAACAACACCTCCAGAATATTTTAATGAAGGTTCGTTATTAAAAGCGATGGAGAACCCTCAGAAC
TTTATTCAATTGAAGGATAAAAAATATGCGCAAACTTTAAAACAAACAGGTGGTATCGGCACAGTTGCAACAAGGGCCGA
CATTATCGATAAATTATTTAATATGAATGCCATTGAATCAAGAGACGGTAAAATTAAAGTAACGTCAAAAGGTAAACAAA
TATTAGAATTAGCACCAGAAGAATTAACGTCGCCACTTTTAACTGCACAATGGGAAGAAAAATTACTTTTAATTGAACGT
GGTAAATATCAGGCGAAAACATTTATTAATGAAATGAAAGATTTTACGAAAGATGTTGTAAATGGGATTAAAAATAGTGA
TCGTAAATATAAACACGATAATTTAACAACCACAGAATGCCCAACGTGTGGTAAATTCATGATTAAAGTTAAAACTAAAA
ATGGTCAGATGCTTGTGTGCCAAGATCCATCTTGTAAGACGAAAAAGAATGTACAGCGCAAAACAAATGCAAGATGTCCA
AACTGTAAAAAGAAATTAACGTTGTTTGGTAAAGGGAAAGAAGCGGTATATCGTTGTGTTTGTGGACATTCTGAAACGCA
AGCACATATGGATCAGCGTATGAAGTCTAAATCCTCTGGTAAAGTATCTCGTAAAGAAATGAAAAAGTATATGAATAAAA
ATGAAGGTTTAGACAATAATCCGTTTAAAGATGCATTAAAGAACTTGAATTTATAG

Upstream 100 bases:

>100_bases
ACATGTTACAATACATTTAACACCATTGAATTTAAATCAAAGATTAGTGGAATAGATGAAGCACGTTCGAAATAAAAGAA
CGTATGAGAAAGGATAATTT

Downstream 100 bases:

>100_bases
ATAAAATCGAACAAAGTTGAATCAGAAAAACGAAAAGTTCGCTTTTGGTATTGTTTTTTATTAAGAATGATATTAAACTA
TTAAGGTATTTTAAAAAAAG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

Sequences:

>Translated_711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
>Mature_711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=675, Percent_Identity=23.7037037037037, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI10835218, Length=713, Percent_Identity=22.1598877980365, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1788061, Length=600, Percent_Identity=29.5, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI1787529, Length=599, Percent_Identity=26.7111853088481, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32563869, Length=685, Percent_Identity=23.7956204379562, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=615, Percent_Identity=21.7886178861789, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323263, Length=480, Percent_Identity=22.9166666666667, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640096, Length=709, Percent_Identity=23.4132581100141, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585251, Length=613, Percent_Identity=23.9804241435563, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP3_STAA3 (Q2FEN5)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_494843.1
- HSSP:   P46799
- ProteinModelPortal:   Q2FEN5
- STRING:   Q2FEN5
- EnsemblBacteria:   EBSTAT00000036403
- GeneID:   3914904
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_2208
- eggNOG:   COG0550
- GeneTree:   EBGT00050000024420
- HOGENOM:   HBG585507
- OMA:   CPECHKK
- ProtClustDB:   PRK07726
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01056

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 81525; Mature: 81525

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00396 TOPOISOMERASE_I_PROK; PS00196 COPPER_BLUE

Important sites: ACT_SITE 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEW
CCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCHHHHHCCHHHH
RLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKV
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
GNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHEEEEC
YDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
CCCCEECCCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHH
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KVVTYPRTDSNYLTTDMVDTMKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDH
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEECCC
TTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
HHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCHH
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPE
HHEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHCCCH
ELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTEC
HHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCV
CCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHCEEEEECCHHHHHHHH
CGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEW
CCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCHHHHHCCHHHH
RLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKV
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
GNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHEEEEC
YDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
CCCCEECCCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHH
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KVVTYPRTDSNYLTTDMVDTMKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDH
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEECCC
TTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
HHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCHH
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPE
HHEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHCCCH
ELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTEC
HHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCV
CCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHCEEEEECCHHHHHHHH
CGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA