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The map label for this gene is pdxT

Identifier: 15926197

GI number: 15926197

Start: 558489

End: 559049

Strand: Direct

Name: pdxT

Synonym: SA0478

Alternate gene names: 15926197

Gene position: 558489-559049 (Clockwise)

Preceding gene: 15926196

Following gene: 15926199

Centisome position: 19.84

GC content: 33.69

Gene sequence:

>561_bases
ATGAAAATAGGTGTATTAGCATTACAAGGTGCAGTACGTGAACATATTAGACATATTGAATTAAGTGGTCATGAAGGTAT
TGCAGTTAAAAAAGTTGAACAATTAGAAGAAATCGAGGGCTTAATATTACCTGGTGGCGAGTCTACAACGTTACGTCGAT
TAATGAATTTATATGGATTTAAAGAGGCTTTACAAAATTCAACTTTACCTATGTTTGGTACATGCGCAGGATTAATAGTT
CTAGCGCAAGATATAGTTGGTGAAGAAGGATACCTTAACAAGTTGAATATTACTGTACAACGAAACTCATTCGGTAGACA
AGTTGACAGCTTTGAAACAGAATTAGATATTAAAGGTATCGCTACAGATATTGAAGGTGTCTTTATAAGAGCGCCACATA
TTGAAAAAGTAGGTCAAGGCGTAGATATCCTATGTAAGGTTAATGAGAAAATTGTAGCTGTCCAGCAAGGTAAATATTTA
GGCGTATCATTCCATCCTGAATTAACAGATGACTATAGAGTAACTGATTACTTTATTAATCATATTGTAAAAAAAGCATA
G

Upstream 100 bases:

>100_bases
AATTGGAAGATTAGCAAGTGAACTTGGCACTGCTATGAAAGGTTTAGATATCAATCAATTATCATTAGAAGAACGTATGC
AAGAGCGTGGTTGGTAAGAT

Downstream 100 bases:

>100_bases
CTTAATGTATGCTAAATCAACGAATTATTGATATTTATAGATTTGTTGAGAAGAAAATATCTCCTTCAAACTTAGCTTTG
GAGGAGTTATTTTTTATGTC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILPGGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIV
LAQDIVGEEGYLNKLNITVQRNSFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
GVSFHPELTDDYRVTDYFINHIVKKA

Sequences:

>Translated_186_residues
MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILPGGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIV
LAQDIVGEEGYLNKLNITVQRNSFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
GVSFHPELTDDYRVTDYFINHIVKKA
>Mature_186_residues
MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILPGGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIV
LAQDIVGEEGYLNKLNITVQRNSFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
GVSFHPELTDDYRVTDYFINHIVKKA

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323995, Length=214, Percent_Identity=37.8504672897196, Blast_Score=118, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321048, Length=214, Percent_Identity=37.8504672897196, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323742, Length=208, Percent_Identity=37.0192307692308, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_STAA1 (A7WYT2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441107.1
- ProteinModelPortal:   A7WYT2
- SMR:   A7WYT2
- STRING:   A7WYT2
- EnsemblBacteria:   EBSTAT00000005196
- GeneID:   5559977
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0517
- eggNOG:   COG0311
- GeneTree:   EBGT00050000024659
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   SAUR418127:SAHV_0517-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20630; Mature: 20630

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 75-75 ACT_SITE 165-165 ACT_SITE 167-167 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILPGGESTTLRRLMNLYGF
CCEEHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
KEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRNSFGRQVDSFETELDIKGI
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCEEE
ATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYLGVSFHPELTDDYRVTDYFIN
EECCCEEEEECCCHHHHCCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHH
HIVKKA
HHHHCC
>Mature Secondary Structure
MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILPGGESTTLRRLMNLYGF
CCEEHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
KEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRNSFGRQVDSFETELDIKGI
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCEEE
ATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYLGVSFHPELTDDYRVTDYFIN
EECCCEEEEECCCHHHHCCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHH
HIVKKA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA