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The map label for this gene is lysS

Identifier: 15926194

GI number: 15926194

Start: 547571

End: 549058

Strand: Direct

Name: lysS

Synonym: SA0475

Alternate gene names: 15926194

Gene position: 547571-549058 (Clockwise)

Preceding gene: 15926193

Following gene: 15926196

Centisome position: 19.45

GC content: 34.95

Gene sequence:

>1488_bases
ATGTCAGAAGAAATGAATGACCAAATGTTGGTTCGACGTCAAAAATTACAAGAATTATATGATCTTGGTATAGACCCGTT
TGGTTCTAAATTTGACCGTTCAGGTTTATCTAGTGATTTGAAAGAAGAGTGGGACCAGTATTCTAAAGAAGAATTGGTAG
AAAAAGAAGCGGATAGTCATGTCGCTATAGCTGGACGATTAATGACTAAGCGTGGTAAAGGTAAAGCAGGATTTGCACAC
GTTCAGGACTTAGCTGGACAAATTCAAATTTACGTTCGTAAAGATCAAGTTGGCGATGACGAATTTGATTTATGGAAAAA
TGCTGATTTAGGCGATATCGTTGGTGTTGAAGGTGTAATGTTCAAAACAAATACTGGCGAATTATCGGTTAAAGCGAAGA
AATTCACGCTACTAACTAAATCATTGCGACCATTACCGGATAAATTCCACGGTTTACAGGATATTGAACAGAGATATCGT
CAAAGATATTTAGATTTAATTACGAACGAAGATAGCACTCGTACATTTATTAATCGTAGTAAAATCATTCAAGAAATGCG
TAATTATTTAAATAATAAAGGTTTCTTGGAAGTAGAAACACCTATGATGCACCAAATTGCTGGTGGCGCAGCTGCTAGAC
CATTTGTAACACATCATAATGCATTAGATGCAACGTTATACATGCGTATTGCTATTGAGTTGCATTTAAAACGTTTAATT
GTCGGTGGACTTGAAAAAGTATATGAAATTGGTAGAGTATTCCGTAATGAAGGTGTATCAACTAGACATAACCCTGAATT
CACAATGATTGAATTATATGAAGCATATGCAGATTATCATGACATTATGGATTTAACAGAATCTATGGTGAGACATATTG
CCAATGAAGTATTAGGTTCTGCAAAAGTACAATACAATGGGGAAACGATTGATTTAGAATCTGCTTGGACTCGTTTGCAT
ATTGTTGATGCTGTAAAAGAAGCTACTGGTGTGGATTTTTATGAAGTTAAAAGTGATGAAGAAGCTAAAGCTTTAGCTAA
AGAACATGGTATTGAAATTAAAGATACAATGAAATATGGTCATATTTTAAATGAATTCTTTGAGCAAAAAGTTGAAGAAA
CACTTATTCAGCCAACGTTTATCTATGGTCATCCGACTGAAATTTCACCTTTAGCGAAGAAAAATCCTGAAGATCCTAGA
TTTACTGATCGTTTCGAATTGTTCATTGTAGGTAGAGAGCATGCAAATGCATTTACTGAATTAAATGATCCTATTGATCA
AAAAGGTCGTTTTGAAGCGCAACTTGTTGAAAAGGCGCAAGGTAATGATGAAGCGCATGAAATGGATGAAGATTACATTG
AAGCGTTAGAATATGGTATGCCTCCGACAGGTGGTCTTGGTATCGGTATTGACAGATTGGTTATGTTATTAACTGATTCT
CCATCAATCAGAGACGTGTTATTATTCCCTTATATGAGACAAAAATAA

Upstream 100 bases:

>100_bases
GAGCAAAAATGTCTGATATTTTATAAAACTTTAAAGGAAAATGTTTGAGTGTACCAGTTGGAATACTAAAGGATTACAAC
AAGTTAAAGGAGAGAAAGTT

Downstream 100 bases:

>100_bases
ATGACGTTGATTGTTAGTAAGAGCTCTCGTGTATACAACATGTGTATGCGAGGGTTTTCTTAATTATGGTAATTAGTTCG
TGTTTGAATGTTTTTGATAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAH
VQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLI
VGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLH
IVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPR
FTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDS
PSIRDVLLFPYMRQK

Sequences:

>Translated_495_residues
MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAH
VQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLI
VGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLH
IVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPR
FTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDS
PSIRDVLLFPYMRQK
>Mature_494_residues
SEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHV
QDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQ
RYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLIV
GGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHI
VDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRF
TDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDSP
SIRDVLLFPYMRQK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=42.5196850393701, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=42.5196850393701, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=497, Percent_Identity=24.1448692152917, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI40789249, Length=255, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1790571, Length=495, Percent_Identity=53.3333333333333, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=52.7272727272727, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=31.4984709480122, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788173, Length=309, Percent_Identity=23.9482200647249, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=44.4672131147541, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=44.4672131147541, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=44.4672131147541, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551876, Length=484, Percent_Identity=23.1404958677686, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=263, Percent_Identity=24.7148288973384, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=43.4442270058708, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=32.7731092436975, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=476, Percent_Identity=24.5798319327731, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321807, Length=464, Percent_Identity=23.9224137931034, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640849, Length=506, Percent_Identity=42.8853754940711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640851, Length=506, Percent_Identity=42.8853754940711, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=484, Percent_Identity=23.7603305785124, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24584738, Length=255, Percent_Identity=28.6274509803922, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56720; Mature: 56589

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSH
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
VAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVM
EEHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEE
FKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRS
EECCCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLI
HHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGS
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYG
CEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHH
HILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTE
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHHHHHHHH
LNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDS
CCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
PSIRDVLLFPYMRQK
CCCCHHHCCCHHCCC
>Mature Secondary Structure 
SEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSH
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
VAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVM
EEHHHHHHHCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEE
FKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRS
EECCCCCEEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLI
HHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGS
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYG
CEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHH
HILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTE
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHHHHHHHH
LNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDS
CCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
PSIRDVLLFPYMRQK
CCCCHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA