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The map label for this gene is folK

Identifier: 15926193

GI number: 15926193

Start: 546556

End: 547032

Strand: Direct

Name: folK

Synonym: SA0474

Alternate gene names: 15926193

Gene position: 546556-547032 (Clockwise)

Preceding gene: 15926192

Following gene: 15926194

Centisome position: 19.42

GC content: 33.75

Gene sequence:

>477_bases
ATGATTCAAGCATACTTAGGATTAGGTAGTAATATTGGTGATAGAGAAAGCCAGTTAAACGATGCTATAAAGATTTTGAA
TGAATATGATGGTATTAGCGTATCTAATATTTCTCCGATTTATGAAACAGCACCAGTTGGGTATACTGAGCAACCTAACT
TTTTAAATTTGTGTGTTGAAATTCAAACAACACTCACAGTATTACAACTGTTGGAATGTTGTTTGAAGACAGAAGAATGT
TTACACCGTATTAGAAAGGAACGATGGGGTCCTAGAACTTTAGATGTGGATATTTTGTTGTATGGAGAAGAAATGATAGA
TTTACCAAAACTGTCGGTGCCACATCCGAGAATGAATGAACGTGCATTTGTTTTAATCCCATTAAATGATATAGCAGCAA
ATGTCGTAGAACCACGTTCGAAATTGAAAGTGAAAGATTTAGTTTTTGTCGATGACAGTGTAAAGAGATATAAATAA

Upstream 100 bases:

>100_bases
ATAATCGTGTAATGGAAACAAAAGTGAGAATCACTAAAGAAAACCCACCGATTCCGGGTCATTATGATGGAGTAGGTATC
GAAATAGTGAGGGAGAATAA

Downstream 100 bases:

>100_bases
TGTATTGTTGAGAACATTCATATTTATTGGGAATAGATGATTGATTATTGGAGATGTTTCCGCATTTTGATTATCTCTAA
ATGCATTTGATTACGAATTA

Product: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridin e pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETAPVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEEC
LHRIRKERWGPRTLDVDILLYGEEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK

Sequences:

>Translated_158_residues
MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETAPVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEEC
LHRIRKERWGPRTLDVDILLYGEEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK
>Mature_158_residues
MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETAPVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEEC
LHRIRKERWGPRTLDVDILLYGEEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=143, Percent_Identity=40.5594405594406, Blast_Score=114, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324073, Length=139, Percent_Identity=42.4460431654676, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18002; Mature: 18002

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETAPVGYTEQPNFLNLCVE
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHH
IQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYGEEMIDLPKLSVPHPRMNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHCCCCCCCCCCCCCC
RAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK
CEEEEEECHHHHHHHHCHHHHCEEEEEEEECCHHHHCC
>Mature Secondary Structure
MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETAPVGYTEQPNFLNLCVE
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHH
IQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYGEEMIDLPKLSVPHPRMNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHCCCCCCCCCCCCCC
RAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK
CEEEEEECHHHHHHHHCHHHHCEEEEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]