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The map label for this gene is rutD

Identifier: 159185242

GI number: 159185242

Start: 2467020

End: 2467805

Strand: Reverse

Name: rutD

Synonym: Atu2497

Alternate gene names: 159185242

Gene position: 2467805-2467020 (Counterclockwise)

Preceding gene: 159185243

Following gene: 15889762

Centisome position: 86.85

GC content: 60.18

Gene sequence:

>786_bases
ATGCATTTCGAGGTTCATGGCCGCACCGATGCCGAGGCGCCCACCATCCTTTTGTCTTCGGGGCTTGGCGGATCGAGTGC
CTATTGGCTGCCGCAGATCGAGGCCCTTTCCGATCATTTCCGGATCGTCACCTATGATCATCGCGGAACCGGCAGGACGG
GCGGGGAGGTGCCGACAGAGGGCGGCATATCTGCCATGGCGGATGATGTGCTGGAGATTGTCTCGGCTCTGAACCTCGAA
AAATTCCACTTCATGGGTCATGCGCTTGGCGGTCTGATCGGCCTCGATATCGCGCTGCGCCAGCCCCGCCTCATCGACAG
GCTTGTGCTCATCAATGCCTGGAGCAAGGCCGATCCGCATTCCGGGCGCTGCTTTGACGTGCGGATCGAGCTTCTGGAGA
AATCCGGTGTCGACGCCTTCGTCAAAGCCCAGCCGCTGTTTCTTTATCCGGCGGCCTGGATGTCGGAACATCAGGAGCGG
CTGGCGCGAGATGATGCTCATGGCGTCGCGCATTTTCAGGGAAAGACCAACGTGCTTCGTCGTATAGCCGCGCTGCGGGC
TTTCGATATCGATGCCCGGCTTGGCGAGATCGGCAATCCGGTGCTGGTGGTGGCGACGAAGGACGATCTTCTGGTGCCCT
ATACGCGCTCGCTGCGTTTGGCGGAGGGATTGCCGCAAAGCGAGCTTTGCCTTTTGGATTTCGGGGCCCACGCGGTCAAT
ATCACCGAGCCTGACCTTTTCAACACCCGGCTGTTGCAGTTCCTGCTGCCAGCCGACCAGACATGA

Upstream 100 bases:

>100_bases
CCGGCGCGGTATTGCATCCAGTGCGGCCTGGTAAAGCCTGATGCACTGGTGGAAATCGCCACCGTCGCCCATATCGGCAA
ATAGGGCGAAGTGGCAGATG

Downstream 100 bases:

>100_bases
TGACAGGATTTTAGAGCAAGATGACGAAATCACTTGATGACGCTGCACTGGCGACGCTTTTCACCGAAGCACGCACCCAC
AATGGTTGGACGGACAAGCC

Product: hydrolase

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTEGGISAMADDVLEIVSALNLE
KFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPHSGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQER
LARDDAHGVAHFQGKTNVLRRIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
ITEPDLFNTRLLQFLLPADQT

Sequences:

>Translated_261_residues
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTEGGISAMADDVLEIVSALNLE
KFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPHSGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQER
LARDDAHGVAHFQGKTNVLRRIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
ITEPDLFNTRLLQFLLPADQT
>Mature_261_residues
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTEGGISAMADDVLEIVSALNLE
KFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPHSGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQER
LARDDAHGVAHFQGKTNVLRRIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
ITEPDLFNTRLLQFLLPADQT

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=239, Percent_Identity=42.6778242677824, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_AGRT5 (Q7CWX3)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_355444.2
- ProteinModelPortal:   Q7CWX3
- STRING:   Q7CWX3
- GeneID:   1134535
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2497
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   FLYPAAW
- ProtClustDB:   CLSK2476192
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- PRINTS:   PR00111
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 28691; Mature: 28691

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTE
CEEEECCCCCCCCCEEEEECCCCCCCEEECCCHHHHCCCEEEEEEECCCCCCCCCCCCCC
GGISAMADDVLEIVSALNLEKFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPH
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCCCC
SGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQERLARDDAHGVAHFQGKTNVLR
CCCEEEEEEEEHHHCCCCEEEECCCEEEECHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
RIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
HHHHHHHHCCCHHHHCCCCCEEEEEECCCEECCCHHCCHHHCCCCCCCEEEEECCCEEEE
ITEPDLFNTRLLQFLLPADQT
CCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTE
CEEEECCCCCCCCCEEEEECCCCCCCEEECCCHHHHCCCEEEEEEECCCCCCCCCCCCCC
GGISAMADDVLEIVSALNLEKFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPH
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCCCC
SGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQERLARDDAHGVAHFQGKTNVLR
CCCEEEEEEEEHHHCCCCEEEECCCEEEECHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
RIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
HHHHHHHHCCCHHHHCCCCCEEEEEECCCEECCCHHCCHHHCCCCCCCEEEEECCCEEEE
ITEPDLFNTRLLQFLLPADQT
CCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194