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The map label for this gene is queF

Identifier: 159185135

GI number: 159185135

Start: 2245350

End: 2245814

Strand: Reverse

Name: queF

Synonym: Atu2273

Alternate gene names: 159185135

Gene position: 2245814-2245350 (Counterclockwise)

Preceding gene: 159185136

Following gene: 159185132

Centisome position: 79.03

GC content: 60.0

Gene sequence:

>465_bases
ATGTCCGTGACGGATGTTTCCGGCCTGTCGCAGCTGGGCACGAAGGTTGATACGCCAGAGAGCCCGGAAAAGGCTGTTCT
CGAAAAAGTGCCAAACGGCAATGCTGGCACCGATTATGTCGTGCGCTTCACCGCGCCCGAATTCACCTCGCTGTGCCCAA
TGACCGGCCAGCCGGATTTCGCCCATATCGTCATCGACTATATTCCGGGCGATTTTCTGGTGGAATCGAAGTCGCTGAAG
CTGTTTCTGCAGTCTTTCCGCAATCATGGCGCATTCCACGAGGACTGCTCGGTCTATATTGCCAAGCGGCTGGTGGAGCT
GCTGCAGCCGAAATGGCTGCGCATCGGCGCCTACTGGTATCCGCGCGGCGGCATTCCGATCGACGTGTTCTGGCAGACGG
GAGCCGCCCCCGAAGGCGTATGGCTGCCGGATCAGGGCGTTGCACCGTATCGGGGTCGCGGCTGA

Upstream 100 bases:

>100_bases
TTCACGTCGAGCCTGAGGGCGAAAAGGCGCATGGCGTCAAAGTCATCGTTTGAACAGTCCGGGTCGCAATGATCCGGTTT
TGAATAATCAGGAGTTTTGC

Downstream 100 bases:

>100_bases
TCCGGCTTTTCAGGCGATTGCAGGCTCAGGCGAACGAACCGTCGTCGCCTGAATCGGAATCCATGTCGTCGGCGCTATCG
TCGTAATCGGCCTGCTGGAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSVTDVSGLSQLGTKVDTPESPEKAVLEKVPNGNAGTDYVVRFTAPEFTSLCPMTGQPDFAHIVIDYIPGDFLVESKSLK
LFLQSFRNHGAFHEDCSVYIAKRLVELLQPKWLRIGAYWYPRGGIPIDVFWQTGAAPEGVWLPDQGVAPYRGRG

Sequences:

>Translated_154_residues
MSVTDVSGLSQLGTKVDTPESPEKAVLEKVPNGNAGTDYVVRFTAPEFTSLCPMTGQPDFAHIVIDYIPGDFLVESKSLK
LFLQSFRNHGAFHEDCSVYIAKRLVELLQPKWLRIGAYWYPRGGIPIDVFWQTGAAPEGVWLPDQGVAPYRGRG
>Mature_153_residues
SVTDVSGLSQLGTKVDTPESPEKAVLEKVPNGNAGTDYVVRFTAPEFTSLCPMTGQPDFAHIVIDYIPGDFLVESKSLKL
FLQSFRNHGAFHEDCSVYIAKRLVELLQPKWLRIGAYWYPRGGIPIDVFWQTGAAPEGVWLPDQGVAPYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_AGRT5 (Q8UD54)

Other databases:

- EMBL:   AE007869
- PIR:   AH2855
- PIR:   E97632
- RefSeq:   NP_355229.2
- ProteinModelPortal:   Q8UD54
- SMR:   Q8UD54
- STRING:   Q8UD54
- GeneID:   1134311
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2273
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- BioCyc:   ATUM176299-1:ATU2273-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17043; Mature: 16911

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTDVSGLSQLGTKVDTPESPEKAVLEKVPNGNAGTDYVVRFTAPEFTSLCPMTGQPDF
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCCCCCCCCE
AHIVIDYIPGDFLVESKSLKLFLQSFRNHGAFHEDCSVYIAKRLVELLQPKWLRIGAYWY
EEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEE
PRGGIPIDVFWQTGAAPEGVWLPDQGVAPYRGRG
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVTDVSGLSQLGTKVDTPESPEKAVLEKVPNGNAGTDYVVRFTAPEFTSLCPMTGQPDF
CCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCCCCCCCCE
AHIVIDYIPGDFLVESKSLKLFLQSFRNHGAFHEDCSVYIAKRLVELLQPKWLRIGAYWY
EEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEE
PRGGIPIDVFWQTGAAPEGVWLPDQGVAPYRGRG
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194