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The map label for this gene is purB [H]

Identifier: 159184943

GI number: 159184943

Start: 1822726

End: 1824033

Strand: Direct

Name: purB [H]

Synonym: Atu1841

Alternate gene names: 159184943

Gene position: 1822726-1824033 (Clockwise)

Preceding gene: 15889142

Following gene: 159184944

Centisome position: 64.14

GC content: 62.23

Gene sequence:

>1308_bases
ATGATCCCTCGTTACTCCCGGCCGGAAATGGTCGCCATCTGGTCGCCGGAAACAAAATTCCGCATCTGGTTCGAGATCGA
GGCCCATGCCTGTGACGCGCTGGCCGAGCTTGGTGTCATCCCGAAGTCAGCAGCCCAGACCATCTGGGAAAAGGGCGGCA
GCGCCACGTTTGACGTCGCCCGCATCGATGAGATCGAGGCCGTGACCAAGCACGACGTCATCGCCTTCCTCACCCATCTC
GCCGAATTTGTCGGCCCCGACAGCCGTTTTGTGCATCAGGGCATGACCTCTTCCGACGTTCTCGACACCACCCTCAACAT
CCAGCTCGTGCGCGCCGCCGATCTGCTGCTCGCCGACATCGACCGGGTGCTCGCGGCTCTCAAGACCCGCGCCTTCGAAC
ACAAGAACACGGTGCGCATCGGCCGCAGCCACGGCATCCATGCCGAGCCGACCACGATGGGCCTGACCTTTGCCCGCTTC
TATGCCGAGATGCACCGTAACCGCGAGCGCCTCGTTAATGCCCGTGCGGAAATCGCCACCGGCGCGATCTCCGGCGCCGT
CGGCACCTTCGCCAATATCGACCCGCGCGTCGAGGAACATGTCTGCGAAAAGCTTGGTCTCATCCCCGAGCCGGTCTCCA
CGCAGGTCATTCCGCGCGATCGCCACGCCATGTTCTTCGCCGTTCTCGGCGTCATCGCCTCGTCGATCGAAAACGTCGCC
ATCGAAATCCGCCACATGCAGCGCACCGAGGTTCTGGAAGCGGAAGAGTTCTTCTCTCCCGGCCAGAAGGGCTCGTCCGC
CATGCCGCACAAACGCAACCCGGTCCTGACCGAAAACCTCACCGGTCTTGCGCGTCTGGTGCGCATGTCCGTCGTGCCGG
CCATGGAGAACGTGGCGCTCTGGCACGAGCGCGATATCAGCCACTCCTCCGTGGAGCGCGCCATCGGCCCCGACACCACG
GTTACGCTGGATTTCGCGCTGAACCGCCTGGCCGGCGTCATCGAGAAGCTGGTGATCTATCCCGACAACATGTTGAAGAA
CATGAACAAGTTCCGCGGCCTCGTACATTCGCAGCGCGTTCTGCTGGCGCTGACACAGGCCGGTGTTTCGCGTGAAGACG
CTTACCGTCTGGTACAGCGCAACGCCATGCGGGTCTGGGAACAGGGTGCCGATTTCCTGGAAGAACTGCTGGGCGACGAG
GAAGTGCGCGCAGCGCTGCCTGAAGACGTGATCCGTGAAAAGTTCGACCTCGGTTATCATACCAAGCACGTCGACACGAT
CTTCACCCGTGTCTTCGGCAAGGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGGCAAGCGACATAGCCATGCAGCTTGATAGATTGAGATTACCGGAACACTTTGCTAAACGGGCAGCAAAATTCCT
CCACTAAGAAAGTCATTGCC

Downstream 100 bases:

>100_bases
TCCAGAACGCGGATAAGTTAATGCTGTCGCGGTCTTGATCGCGACGGCATCAAAGCACAGATATGGGCGCATGAAAGTTT
CAGACGTAGATATCCTCATC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAQTIWEKGGSATFDVARIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFVHQGMTSSDVLDTTLNIQLVRAADLLLADIDRVLAALKTRAFEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMHRNRERLVNARAEIATGAISGAVGTFANIDPRVEEHVCEKLGLIPEPVSTQVIPRDRHAMFFAVLGVIASSIENVA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDTT
VTLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMRVWEQGADFLEELLGDE
EVRAALPEDVIREKFDLGYHTKHVDTIFTRVFGKA

Sequences:

>Translated_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAQTIWEKGGSATFDVARIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFVHQGMTSSDVLDTTLNIQLVRAADLLLADIDRVLAALKTRAFEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMHRNRERLVNARAEIATGAISGAVGTFANIDPRVEEHVCEKLGLIPEPVSTQVIPRDRHAMFFAVLGVIASSIENVA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDTT
VTLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMRVWEQGADFLEELLGDE
EVRAALPEDVIREKFDLGYHTKHVDTIFTRVFGKA
>Mature_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAQTIWEKGGSATFDVARIDEIEAVTKHDVIAFLTHL
AEFVGPDSRFVHQGMTSSDVLDTTLNIQLVRAADLLLADIDRVLAALKTRAFEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMHRNRERLVNARAEIATGAISGAVGTFANIDPRVEEHVCEKLGLIPEPVSTQVIPRDRHAMFFAVLGVIASSIENVA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVVPAMENVALWHERDISHSSVERAIGPDTT
VTLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMRVWEQGADFLEELLGDE
EVRAALPEDVIREKFDLGYHTKHVDTIFTRVFGKA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=404, Percent_Identity=28.7128712871287, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.6089238845144, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1787376, Length=349, Percent_Identity=28.9398280802292, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=394, Percent_Identity=25.8883248730964, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=358, Percent_Identity=25.9776536312849, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=417, Percent_Identity=26.8585131894484, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=26.2376237623762, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI78710009, Length=209, Percent_Identity=27.7511961722488, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48682; Mature: 48682

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAQTIWEKGGSATFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEHH
RIDEIEAVTKHDVIAFLTHLAEFVGPDSRFVHQGMTSSDVLDTTLNIQLVRAADLLLADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHCEEEHHHHHHHHHHH
DRVLAALKTRAFEHKNTVRIGRSHGIHAEPTTMGLTFARFYAEMHRNRERLVNARAEIAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCEKLGLIPEPVSTQVIPRDRHAMFFAVLGVIASSIENVA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERAIGPDTTVTLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMRVWEQGADFLEELLGDEEVRAALPEDVIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCC
TKHVDTIFTRVFGKA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAQTIWEKGGSATFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEHH
RIDEIEAVTKHDVIAFLTHLAEFVGPDSRFVHQGMTSSDVLDTTLNIQLVRAADLLLADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHCEEEHHHHHHHHHHH
DRVLAALKTRAFEHKNTVRIGRSHGIHAEPTTMGLTFARFYAEMHRNRERLVNARAEIAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCEKLGLIPEPVSTQVIPRDRHAMFFAVLGVIASSIENVA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERAIGPDTTVTLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMRVWEQGADFLEELLGDEEVRAALPEDVIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCC
TKHVDTIFTRVFGKA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]