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The map label for this gene is rhtB [C]

Identifier: 159184513

GI number: 159184513

Start: 875273

End: 875917

Strand: Direct

Name: rhtB [C]

Synonym: Atu0879

Alternate gene names: 159184513

Gene position: 875273-875917 (Clockwise)

Preceding gene: 159184511

Following gene: 159184514

Centisome position: 30.8

GC content: 59.38

Gene sequence:

>645_bases
ATGAATTACGCTGAAAATCTCTGGCTTTTCTTCCTGCTCCTGTTCGGCATCATCATCCTGCCGGGCATGGACATGATGTT
TGTGCTGGCAAGCGCGCTGACCGGCGGCAGGAAAACGGGGCTTTCTGCGGCAAGCGGCATGAGTGCTGGCGGCATGGTGC
ATTCTCTTTATGGAGCCGCCGGTGTCGGGTTGCTCGCCACCTGGCTGCCATCTCTGTTTCTGCCGCTTCTTGTCGGCGGG
GCTGCCTGTATGGTCTGGATCGGCTTCGGCCTGATGCGCAGCGCCATCACCGTCAATGGCGATGAGGCGCAGGCAAGCAC
CTCTGCCAGAAAGGCATTCTGGAGGGCGGTGATTACCTGCCTTTCCAATCCCAAAGCCTATCTTTTCATGATGGCGGTCT
ATCCGCAATTCATCAAACCCGGCTTCGGGCCGATCTGGATGCAGGGGTTGGTGATGGGGGCAATGGTGGCCGTGACGCAG
TTTGCCGTCTATGGAACCGTGGCCCTGACGGCCGACAGAAGCCGCGCCTGGCTTATCTCCTCGCCCGCTGCCACGATTTT
CATCGGCCGCGCCGCAGGCTTTCTGCTGATTGCCGCCGCCCTCTTGACTTTTTGGGAGGCATTTTCCTGGAGCATGGGCG
AATGA

Upstream 100 bases:

>100_bases
GACACAACGTTGTCAGTTGGCATTTTTTATAAGCGGAAAAACACATTGAGAAACAAATGCTTTCCCGAATGTGTCATCAT
CTTCTTTAAGGGATCCTGAC

Downstream 100 bases:

>100_bases
TTGAGGATGTCGCGCAATTTCAGCCAGCCGTCAGATACGTGCCATAAAGCCAATCCCGGCCCCGCACCGGAGCCTCGTCT
AGACTTCTGTTTTCACGGCG

Product: RhtB family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNYAENLWLFFLLLFGIIILPGMDMMFVLASALTGGRKTGLSAASGMSAGGMVHSLYGAAGVGLLATWLPSLFLPLLVGG
AACMVWIGFGLMRSAITVNGDEAQASTSARKAFWRAVITCLSNPKAYLFMMAVYPQFIKPGFGPIWMQGLVMGAMVAVTQ
FAVYGTVALTADRSRAWLISSPAATIFIGRAAGFLLIAAALLTFWEAFSWSMGE

Sequences:

>Translated_214_residues
MNYAENLWLFFLLLFGIIILPGMDMMFVLASALTGGRKTGLSAASGMSAGGMVHSLYGAAGVGLLATWLPSLFLPLLVGG
AACMVWIGFGLMRSAITVNGDEAQASTSARKAFWRAVITCLSNPKAYLFMMAVYPQFIKPGFGPIWMQGLVMGAMVAVTQ
FAVYGTVALTADRSRAWLISSPAATIFIGRAAGFLLIAAALLTFWEAFSWSMGE
>Mature_214_residues
MNYAENLWLFFLLLFGIIILPGMDMMFVLASALTGGRKTGLSAASGMSAGGMVHSLYGAAGVGLLATWLPSLFLPLLVGG
AACMVWIGFGLMRSAITVNGDEAQASTSARKAFWRAVITCLSNPKAYLFMMAVYPQFIKPGFGPIWMQGLVMGAMVAVTQ
FAVYGTVALTADRSRAWLISSPAATIFIGRAAGFLLIAAALLTFWEAFSWSMGE

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788099, Length=135, Percent_Identity=31.8518518518519, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22760; Mature: 22760

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYAENLWLFFLLLFGIIILPGMDMMFVLASALTGGRKTGLSAASGMSAGGMVHSLYGAA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHH
GVGLLATWLPSLFLPLLVGGAACMVWIGFGLMRSAITVNGDEAQASTSARKAFWRAVITC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSNPKAYLFMMAVYPQFIKPGFGPIWMQGLVMGAMVAVTQFAVYGTVALTADRSRAWLIS
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
SPAATIFIGRAAGFLLIAAALLTFWEAFSWSMGE
CCCHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNYAENLWLFFLLLFGIIILPGMDMMFVLASALTGGRKTGLSAASGMSAGGMVHSLYGAA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHH
GVGLLATWLPSLFLPLLVGGAACMVWIGFGLMRSAITVNGDEAQASTSARKAFWRAVITC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LSNPKAYLFMMAVYPQFIKPGFGPIWMQGLVMGAMVAVTQFAVYGTVALTADRSRAWLIS
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
SPAATIFIGRAAGFLLIAAALLTFWEAFSWSMGE
CCCHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]