Click here to switch to the map view.

The map label for this gene is pyrE

Identifier: 159184293

GI number: 159184293

Start: 394837

End: 395535

Strand: Direct

Name: pyrE

Synonym: Atu0400

Alternate gene names: 159184293

Gene position: 394837-395535 (Clockwise)

Preceding gene: 159184292

Following gene: 159184294

Centisome position: 13.89

GC content: 57.8

Gene sequence:

>699_bases
ATGAACCATTTCACATTCACTGACCGCACCGTCGTCGCGGAACTGGTTGCGAAGATGTTGTGGGAAATCAAGGCGGTCCA
TTTCAATTCCGAAAGCCCCTACACCTTCGCTTCGGGCATGAAGAGCCCCGTTTATATCGACATGCGCAAGCTCATCTCCT
TCCCGCGCATCCGCTCCGCAGCCATGGATTTTGCGGCAGCGGCTGTCATGCGGGAGGCCGGTTTCGAGAAATTCGACTGT
GTGGCCGGTGGTGAAACGGCCGGCATTCCCTTCGCCGCTTTCCTTGCCGAGCGTCTTGGCCTGCCGATGATCTATTGCCG
CAAAAAGCCGAAAGGTCACGGCCGCAACGCCCAGATCGAAGGCCATATGCCTGATGGTGCTCGCGTTCTCGTTATCGAGG
ATCTGACGACGGCTGGCGGCTCGATGTTCACCTTCATCGACGCCATCCGCGCGGCTGGCGGCATTGTCGATCATGGTATC
GCGCTCTTCTATTACGGCATCTTTGAAGAAGCCGAAGCGCGTTTCGCCAACGGCAATGTCAAGCTGCACTATCTGACGAC
CTGGCGCGATGTTCTGGCTGTCGCCCGGGCTGAAAAACTGTTCGATGAAAAGACGCTGTCGGGGGTTGAAGCCTTCCTCG
ATAATCCGCTGCCCTGGTCGGCGAAGCACGGCGGTGTCAGCGAATTGCCGCAATCGTGA

Upstream 100 bases:

>100_bases
GGCCTGCGGGGGTACGAAATACCAAACAAAGATGTCGTGCTTTCCGAACACCGATGCGGGTTTCGGAGCGATAAACCAAG
GCCGAAAAAGGAGAACGCCT

Downstream 100 bases:

>100_bases
TTTCGGATGCGGCCAGTCCAGCTTGTGGCTGGCCTTCCGATCGATCTTCGTCTAAATCGATTTAAGTTCATTTGATTTGC
TGTTTTGAGGAGGACCGTAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNHFTFTDRTVVAELVAKMLWEIKAVHFNSESPYTFASGMKSPVYIDMRKLISFPRIRSAAMDFAAAAVMREAGFEKFDC
VAGGETAGIPFAAFLAERLGLPMIYCRKKPKGHGRNAQIEGHMPDGARVLVIEDLTTAGGSMFTFIDAIRAAGGIVDHGI
ALFYYGIFEEAEARFANGNVKLHYLTTWRDVLAVARAEKLFDEKTLSGVEAFLDNPLPWSAKHGGVSELPQS

Sequences:

>Translated_232_residues
MNHFTFTDRTVVAELVAKMLWEIKAVHFNSESPYTFASGMKSPVYIDMRKLISFPRIRSAAMDFAAAAVMREAGFEKFDC
VAGGETAGIPFAAFLAERLGLPMIYCRKKPKGHGRNAQIEGHMPDGARVLVIEDLTTAGGSMFTFIDAIRAAGGIVDHGI
ALFYYGIFEEAEARFANGNVKLHYLTTWRDVLAVARAEKLFDEKTLSGVEAFLDNPLPWSAKHGGVSELPQS
>Mature_232_residues
MNHFTFTDRTVVAELVAKMLWEIKAVHFNSESPYTFASGMKSPVYIDMRKLISFPRIRSAAMDFAAAAVMREAGFEKFDC
VAGGETAGIPFAAFLAERLGLPMIYCRKKPKGHGRNAQIEGHMPDGARVLVIEDLTTAGGSMFTFIDAIRAAGGIVDHGI
ALFYYGIFEEAEARFANGNVKLHYLTTWRDVLAVARAEKLFDEKTLSGVEAFLDNPLPWSAKHGGVSELPQS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=208, Percent_Identity=27.8846153846154, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17508631, Length=160, Percent_Identity=33.125, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17933654, Length=202, Percent_Identity=25.7425742574257, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_AGRT5 (Q8UI98)

Other databases:

- EMBL:   AE007869
- PIR:   AG2625
- PIR:   F97407
- RefSeq:   NP_353430.2
- ProteinModelPortal:   Q8UI98
- SMR:   Q8UI98
- STRING:   Q8UI98
- GeneID:   1132438
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0400
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- PhylomeDB:   Q8UI98
- ProtClustDB:   PRK00455
- BioCyc:   ATUM176299-1:ATU0400-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 25514; Mature: 25514

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 107-107 BINDING 108-108 BINDING 111-111 BINDING 113-113 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHFTFTDRTVVAELVAKMLWEIKAVHFNSESPYTFASGMKSPVYIDMRKLISFPRIRSA
CCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCCCCCCEEEEHHHHHCCHHHHHH
AMDFAAAAVMREAGFEKFDCVAGGETAGIPFAAFLAERLGLPMIYCRKKPKGHGRNAQIE
HHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEE
GHMPDGARVLVIEDLTTAGGSMFTFIDAIRAAGGIVDHGIALFYYGIFEEAEARFANGNV
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCE
KLHYLTTWRDVLAVARAEKLFDEKTLSGVEAFLDNPLPWSAKHGGVSELPQS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCC
>Mature Secondary Structure
MNHFTFTDRTVVAELVAKMLWEIKAVHFNSESPYTFASGMKSPVYIDMRKLISFPRIRSA
CCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHCCCCCCEEEEHHHHHCCHHHHHH
AMDFAAAAVMREAGFEKFDCVAGGETAGIPFAAFLAERLGLPMIYCRKKPKGHGRNAQIE
HHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEE
GHMPDGARVLVIEDLTTAGGSMFTFIDAIRAAGGIVDHGIALFYYGIFEEAEARFANGNV
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCE
KLHYLTTWRDVLAVARAEKLFDEKTLSGVEAFLDNPLPWSAKHGGVSELPQS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194