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The map label for this gene is pyrC

Identifier: 159184292

GI number: 159184292

Start: 393648

End: 394685

Strand: Direct

Name: pyrC

Synonym: Atu0399

Alternate gene names: 159184292

Gene position: 393648-394685 (Clockwise)

Preceding gene: 15887747

Following gene: 159184293

Centisome position: 13.85

GC content: 60.6

Gene sequence:

>1038_bases
ATGAAGTCGCTCACCCTGCGCCGCCCTGATGACTGGCACCTGCATCTTCGTGATGGCGCCATGCTGGAAGGGGTGATCGG
GGATACGAGCCGCCATTTCGCCCGCGCCATCATCATGCCGAATCTGGTGCCGCCCGTCGTCACCACGGCCGATGCCAGCG
CCTATCGAGAACGCATCCTCAAGGCGATCCCGGAGGGTGACCGCTTCGAGCCGCTGATGACCCTCTACCTCACTGAGGAT
ACTGTTGCTGATGACGTGGAAGAGGGCAAAAAAAGCGGCCTCATCACCGCCGTAAAGCTCTATCCTGCGGGTGCGACCAC
CAATTCTCATGGCGGCGTGCGCGATTTCAACAAGGCGATGCCGGTGCTGGAACGCATGGCAAAGATCGGCCTGCCGCTCT
GCGTGCATGGCGAGGTGACGACGCCAGACGTCGATATTTTCGACCGCGAGAAGGCCTTCATCGATACGGTTCTGGAGCCC
CTGCGCCAGCGCCTGCCAGAGTTGAAGGTGACGATGGAACACATCACCACCCGCGATGGCGTGGACTACATCAAGTCTTC
CAATGCCAATCTGGCCGGCTCCATCACCACGCACCACCTCATCATCAACCGCAACGCCATTCTGGTCGGCGGTATCAAGC
CGCATTATTATTGCCTGCCCGTCGCCAAGCGCGAGGAGCATCGTCTGGCGCTGCGGGCTGCTGCGACGTCGGGTGATGCC
CGCTTCTTCCTCGGCACGGATTCCGCCCCACATGTGGACCCGCTGAAAGAATGCGCCTGCGGCTGTGCTGGCATCTATAC
TTCCATCAACACCATGAGCTGCCTTGCCCATGTCTTCGAGGATGAGAATGCCCTCGATAAGCTGGAGGCCTTCGCCTCGC
TCAACGGCCCGGCCTGGTATGGTCTTGCGCCGAACGACGAGACGATCACGCTGGTCAAGCGCGAGGAAGCGGTGGCGTTT
CCCGAAAAGATCGAAACGGGCGCCGGGCCGGTGACCGTATTCGATCCGATGTTCCCGCTTCATTGGGATGTAAACTGA

Upstream 100 bases:

>100_bases
AAAAATGACATTTACAGCTGCCGGATGGTCTCTGGCGGCTGTTTTCCCGCGACAAAAGATGCTAAGCGGGCGCAATTCCA
TCATGTTCCGAGGTATCCCG

Downstream 100 bases:

>100_bases
TTTTTATGACCGCCGGCACTGCCGGCGGTTTTGTTATGATCCACGGGCATTGGCCTGCGGGGGTACGAAATACCAAACAA
AGATGTCGTGCTTTCCGAAC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKSLTLRRPDDWHLHLRDGAMLEGVIGDTSRHFARAIIMPNLVPPVVTTADASAYRERILKAIPEGDRFEPLMTLYLTED
TVADDVEEGKKSGLITAVKLYPAGATTNSHGGVRDFNKAMPVLERMAKIGLPLCVHGEVTTPDVDIFDREKAFIDTVLEP
LRQRLPELKVTMEHITTRDGVDYIKSSNANLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREEHRLALRAAATSGDA
RFFLGTDSAPHVDPLKECACGCAGIYTSINTMSCLAHVFEDENALDKLEAFASLNGPAWYGLAPNDETITLVKREEAVAF
PEKIETGAGPVTVFDPMFPLHWDVN

Sequences:

>Translated_345_residues
MKSLTLRRPDDWHLHLRDGAMLEGVIGDTSRHFARAIIMPNLVPPVVTTADASAYRERILKAIPEGDRFEPLMTLYLTED
TVADDVEEGKKSGLITAVKLYPAGATTNSHGGVRDFNKAMPVLERMAKIGLPLCVHGEVTTPDVDIFDREKAFIDTVLEP
LRQRLPELKVTMEHITTRDGVDYIKSSNANLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREEHRLALRAAATSGDA
RFFLGTDSAPHVDPLKECACGCAGIYTSINTMSCLAHVFEDENALDKLEAFASLNGPAWYGLAPNDETITLVKREEAVAF
PEKIETGAGPVTVFDPMFPLHWDVN
>Mature_345_residues
MKSLTLRRPDDWHLHLRDGAMLEGVIGDTSRHFARAIIMPNLVPPVVTTADASAYRERILKAIPEGDRFEPLMTLYLTED
TVADDVEEGKKSGLITAVKLYPAGATTNSHGGVRDFNKAMPVLERMAKIGLPLCVHGEVTTPDVDIFDREKAFIDTVLEP
LRQRLPELKVTMEHITTRDGVDYIKSSNANLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREEHRLALRAAATSGDA
RFFLGTDSAPHVDPLKECACGCAGIYTSINTMSCLAHVFEDENALDKLEAFASLNGPAWYGLAPNDETITLVKREEAVAF
PEKIETGAGPVTVFDPMFPLHWDVN

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=318, Percent_Identity=56.9182389937107, Blast_Score=374, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323452, Length=342, Percent_Identity=34.7953216374269, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_AGRT5 (Q8UI99)

Other databases:

- EMBL:   AE007869
- PIR:   AF2625
- PIR:   E97407
- RefSeq:   NP_353429.2
- ProteinModelPortal:   Q8UI99
- SMR:   Q8UI99
- STRING:   Q8UI99
- GeneID:   1132437
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0399
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q8UI99
- ProtClustDB:   PRK05451
- BioCyc:   ATUM176299-1:ATU0399-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 37951; Mature: 37951

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTLRRPDDWHLHLRDGAMLEGVIGDTSRHFARAIIMPNLVPPVVTTADASAYRERIL
CCCCCCCCCCCCEEEEECCCEEEHHHCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
KAIPEGDRFEPLMTLYLTEDTVADDVEEGKKSGLITAVKLYPAGATTNSHGGVRDFNKAM
HHCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHH
PVLERMAKIGLPLCVHGEVTTPDVDIFDREKAFIDTVLEPLRQRLPELKVTMEHITTRDG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VDYIKSSNANLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREEHRLALRAAATSGDA
HHHHHCCCCCEEECEEEEEEEEECCEEEECCCCCCEEEEECCCCCCHHEEEEEECCCCCE
RFFLGTDSAPHVDPLKECACGCAGIYTSINTMSCLAHVFEDENALDKLEAFASLNGPAWY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
GLAPNDETITLVKREEAVAFPEKIETGAGPVTVFDPMFPLHWDVN
EECCCCCEEEEEECCCHHCCHHHHCCCCCCEEEECCCCCEEECCC
>Mature Secondary Structure
MKSLTLRRPDDWHLHLRDGAMLEGVIGDTSRHFARAIIMPNLVPPVVTTADASAYRERIL
CCCCCCCCCCCCEEEEECCCEEEHHHCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
KAIPEGDRFEPLMTLYLTEDTVADDVEEGKKSGLITAVKLYPAGATTNSHGGVRDFNKAM
HHCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHH
PVLERMAKIGLPLCVHGEVTTPDVDIFDREKAFIDTVLEPLRQRLPELKVTMEHITTRDG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VDYIKSSNANLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREEHRLALRAAATSGDA
HHHHHCCCCCEEECEEEEEEEEECCEEEECCCCCCEEEEECCCCCCHHEEEEEECCCCCE
RFFLGTDSAPHVDPLKECACGCAGIYTSINTMSCLAHVFEDENALDKLEAFASLNGPAWY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
GLAPNDETITLVKREEAVAFPEKIETGAGPVTVFDPMFPLHWDVN
EECCCCCEEEEEECCCHHCCHHHHCCCCCCEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194