Click here to switch to the map view.

The map label for this gene is barA [H]

Identifier: 159184266

GI number: 159184266

Start: 333482

End: 335722

Strand: Direct

Name: barA [H]

Synonym: Atu0343

Alternate gene names: 159184266

Gene position: 333482-335722 (Clockwise)

Preceding gene: 15887692

Following gene: 159184267

Centisome position: 11.74

GC content: 61.31

Gene sequence:

>2241_bases
ATGAGCGAACTTGCACGACTTCGGCAAAAAGTTTCAGATGGTCTCGGTCTGGCCACCCCAGCGGCGGATCCTGCCGATAA
GACAACGCGCCTTGCGAACGAGGAACATGCCAGCGATGTCAGGGCGGTTGCGCTTTATGTCGCCATTTCGCTATCGGTGG
GGGCCTTCGCTGCCGCCCTGATCGCACTTGCGGTGCCCTATGCCGTCACCGTCGTGCTCGCCTGTTTTTTTGGCGCGGTG
CTTATGGGTGCCCTGTTTTATGCGCTCTTCGGCAAATCTTTCCCGCTTCCCGGCACGCTGCCCGCAGATTATTCGGATGT
CCGGCTGCGGCAAACGCAAAACCGGTCGCTGATTGCCGAAATGCAGGAGTCCATGGGCGATATCGTCGTGATCCGCAACA
TGAACCGGCGCATCGTCGAGGCGAACCGCGTCTTTCGCGAAATGACCGGCTGCACGTCGCCGGAAGGCTTGAGCTGCGAG
GAGATCGGCATTGCTTTCCGCCCGGGTGACAGGGTCCATGCCTATGATGTGGAGATCGCGACTCCGTTCGGTCAGCGCAT
CTTCTCCTGGCACGATGTGGTAACCCGCGATCTCGCAAGCAGCCGTCTCATGATCAGCAGTATCGCAAGAGACGTGACCG
AAGAACGGCTTGCGCTTGCCGAACGGGAAGATGCCCGCCTGCGCGCCGAAAAGGCCGATGCCGAAAAGGCGCGGTTGCTT
GCCACGGTCAGCCATGAAATCCGCCTGCCGCTCTCCGGCATGCTCGGCATGAACCACCTTCTGTCGCAGACGAAGCTCAA
TCAGGAACAGCGTAATTATCTGGATGGCATGAAACAGTCCGGTCAGTCGCTGGTGCAGCTTGTCGAGGATCTCCTGGATT
ATTCGACCATGGAAGCCGGCCGCTTCAGGCTCAACCCGCGCGCGGAAAACCTGCGGCGGCTGATCGAGAGCATCGTGGAG
ATGCTTGCCCATCGCGCCCATGAAAAGGGCATCGAAATCGCCTCCTTCGTCACGCCTGACGTGCCCGATTATCTCGATTT
TGATCCGGCAAGGCTGCGCCAGGTCCTGTTCAATCTCATCGGCAATGCCGTGAAATTCACGCAGGTGGGCGGGGTCGTTA
TCCGGGCGCGCATGGTCGACGGCGAATTGCAGATCACCGTCGAAGATACCGGCCCCGGCATGAGTGAGGCGGAGCAGGCG
CGTATCTTCGGGGAGTTCGAGCAGGCCGGCCCGCTGTCGCAACGAAGCGCCGGCACGGGGCTGGGACTTGCGATCTCCGC
CCGCATCGTGCGGGAATTCGGCGGCAACCTCACGGTTTCGAGCCGCAGGGGTGAGGGCAGCACCTTCAAGCTGGTGTTCC
GCCCGGGCAGGGCTCCCGCCGAGATGCCGGATTCCGGCCGGAGCCATTGCCTGCAGCACACCAATGTCCTGCTGATTGCG
CCGGAGGGTGTGGCGGCGGCGGTGACGATGGAAGCCATCGAGGCTTTCGGTGGCAAGTGTATTCTCATCCATCAGCCGGA
GGATCTGGACAGGCTGAGGAATGAACCTGTCAGCCGGACCGCTGATCTCACCGACATCATCGTCGATCTGCGTATCTCGG
CACTATTCAGGGATGCCCTGCGGGATTGGCCGCAGCAGACGATCCGTCGCACGCTGCTCGTCAGTCCCGAAGAGCGGGCT
TCGACCACCCATGCGTCCTTCGACGCCTGGCTGATCCGGCCCCTTCGCGAAAAATCCCTGATCGATGTGCTGTGTGGCCG
CCTGCGCGGCATAGAGCGGCGCGACGCGATCAACGATAACCACCCGGATGTGGGGTTTTCGTCAAGGGTGGTGGAGAGAG
CGAGCGGCGTGGAGATACTCGTGGCCGAAGACGATGCGGTCAATGCGCGCATCATCCGGGCTGTCCTGGAAAAAAGCGGC
TATATCGTCCGCGCCGTGGACAATTTTCAGGCCCTCCAGCAATCGGTGGCGTCGGGTGCGTCCCGCCTGCCAAATCTCAT
CGTCTCCGATCTTAATATGCCGGGCGGTGAGGGCCTCGACGTGTTGCCGGACCTCCTCGGCAAGCTGAAAGATGAAAGAA
ATATTCCCGTCATCATTCTCAGCGGGGACGCCGGTGCCGGGACCGCCGAGATACTGCTGAAGGCCGGTGCAGGTGCTGTT
TTGGCCAAGCCCGCCGACCCGCGCCGGCTGGTTGAGGAAATCCGTCGCCTATTGGAGGCGAAACGCGTGTTGTCATCATA
A

Upstream 100 bases:

>100_bases
GTCATTCTCGATGAATTTCTGCATGCCCGGGCGGCCAAAAAGTAAAATTTTAGCGTTTCAGCCAAACATATCAAAACCGC
TGCCGTGGTAGCGGTTATGT

Downstream 100 bases:

>100_bases
CTTGACAGACTCGCGTTGGCGGCATTTTGTCACTATTCTGTCGCGAATTAGTGATTTATGACAGGAAAACTCTTTGGTGG
CGGAGCGATTCCCCATGGTT

Product: two component sensor kinase/response regulator hybrid

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MSELARLRQKVSDGLGLATPAADPADKTTRLANEEHASDVRAVALYVAISLSVGAFAAALIALAVPYAVTVVLACFFGAV
LMGALFYALFGKSFPLPGTLPADYSDVRLRQTQNRSLIAEMQESMGDIVVIRNMNRRIVEANRVFREMTGCTSPEGLSCE
EIGIAFRPGDRVHAYDVEIATPFGQRIFSWHDVVTRDLASSRLMISSIARDVTEERLALAEREDARLRAEKADAEKARLL
ATVSHEIRLPLSGMLGMNHLLSQTKLNQEQRNYLDGMKQSGQSLVQLVEDLLDYSTMEAGRFRLNPRAENLRRLIESIVE
MLAHRAHEKGIEIASFVTPDVPDYLDFDPARLRQVLFNLIGNAVKFTQVGGVVIRARMVDGELQITVEDTGPGMSEAEQA
RIFGEFEQAGPLSQRSAGTGLGLAISARIVREFGGNLTVSSRRGEGSTFKLVFRPGRAPAEMPDSGRSHCLQHTNVLLIA
PEGVAAAVTMEAIEAFGGKCILIHQPEDLDRLRNEPVSRTADLTDIIVDLRISALFRDALRDWPQQTIRRTLLVSPEERA
STTHASFDAWLIRPLREKSLIDVLCGRLRGIERRDAINDNHPDVGFSSRVVERASGVEILVAEDDAVNARIIRAVLEKSG
YIVRAVDNFQALQQSVASGASRLPNLIVSDLNMPGGEGLDVLPDLLGKLKDERNIPVIILSGDAGAGTAEILLKAGAGAV
LAKPADPRRLVEEIRRLLEAKRVLSS

Sequences:

>Translated_746_residues
MSELARLRQKVSDGLGLATPAADPADKTTRLANEEHASDVRAVALYVAISLSVGAFAAALIALAVPYAVTVVLACFFGAV
LMGALFYALFGKSFPLPGTLPADYSDVRLRQTQNRSLIAEMQESMGDIVVIRNMNRRIVEANRVFREMTGCTSPEGLSCE
EIGIAFRPGDRVHAYDVEIATPFGQRIFSWHDVVTRDLASSRLMISSIARDVTEERLALAEREDARLRAEKADAEKARLL
ATVSHEIRLPLSGMLGMNHLLSQTKLNQEQRNYLDGMKQSGQSLVQLVEDLLDYSTMEAGRFRLNPRAENLRRLIESIVE
MLAHRAHEKGIEIASFVTPDVPDYLDFDPARLRQVLFNLIGNAVKFTQVGGVVIRARMVDGELQITVEDTGPGMSEAEQA
RIFGEFEQAGPLSQRSAGTGLGLAISARIVREFGGNLTVSSRRGEGSTFKLVFRPGRAPAEMPDSGRSHCLQHTNVLLIA
PEGVAAAVTMEAIEAFGGKCILIHQPEDLDRLRNEPVSRTADLTDIIVDLRISALFRDALRDWPQQTIRRTLLVSPEERA
STTHASFDAWLIRPLREKSLIDVLCGRLRGIERRDAINDNHPDVGFSSRVVERASGVEILVAEDDAVNARIIRAVLEKSG
YIVRAVDNFQALQQSVASGASRLPNLIVSDLNMPGGEGLDVLPDLLGKLKDERNIPVIILSGDAGAGTAEILLKAGAGAV
LAKPADPRRLVEEIRRLLEAKRVLSS
>Mature_745_residues
SELARLRQKVSDGLGLATPAADPADKTTRLANEEHASDVRAVALYVAISLSVGAFAAALIALAVPYAVTVVLACFFGAVL
MGALFYALFGKSFPLPGTLPADYSDVRLRQTQNRSLIAEMQESMGDIVVIRNMNRRIVEANRVFREMTGCTSPEGLSCEE
IGIAFRPGDRVHAYDVEIATPFGQRIFSWHDVVTRDLASSRLMISSIARDVTEERLALAEREDARLRAEKADAEKARLLA
TVSHEIRLPLSGMLGMNHLLSQTKLNQEQRNYLDGMKQSGQSLVQLVEDLLDYSTMEAGRFRLNPRAENLRRLIESIVEM
LAHRAHEKGIEIASFVTPDVPDYLDFDPARLRQVLFNLIGNAVKFTQVGGVVIRARMVDGELQITVEDTGPGMSEAEQAR
IFGEFEQAGPLSQRSAGTGLGLAISARIVREFGGNLTVSSRRGEGSTFKLVFRPGRAPAEMPDSGRSHCLQHTNVLLIAP
EGVAAAVTMEAIEAFGGKCILIHQPEDLDRLRNEPVSRTADLTDIIVDLRISALFRDALRDWPQQTIRRTLLVSPEERAS
TTHASFDAWLIRPLREKSLIDVLCGRLRGIERRDAINDNHPDVGFSSRVVERASGVEILVAEDDAVNARIIRAVLEKSGY
IVRAVDNFQALQQSVASGASRLPNLIVSDLNMPGGEGLDVLPDLLGKLKDERNIPVIILSGDAGAGTAEILLKAGAGAVL
AKPADPRRLVEEIRRLLEAKRVLSS

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=526, Percent_Identity=29.0874524714829, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI48994928, Length=416, Percent_Identity=29.0865384615385, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI87081816, Length=314, Percent_Identity=35.3503184713376, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693157, Length=564, Percent_Identity=26.9503546099291, Blast_Score=156, Evalue=6e-39,
Organism=Escherichia coli, GI1788713, Length=272, Percent_Identity=31.6176470588235, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=30.952380952381, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=27.9352226720648, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=30.7359307359307, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786783, Length=217, Percent_Identity=27.1889400921659, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=430, Percent_Identity=23.7209302325581, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87082128, Length=254, Percent_Identity=27.9527559055118, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1788393, Length=248, Percent_Identity=26.2096774193548, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=27.9816513761468, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790300, Length=258, Percent_Identity=27.1317829457364, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1790346, Length=208, Percent_Identity=29.3269230769231, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1788279, Length=197, Percent_Identity=25.3807106598985, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81573; Mature: 81441

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELARLRQKVSDGLGLATPAADPADKTTRLANEEHASDVRAVALYVAISLSVGAFAAAL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALAVPYAVTVVLACFFGAVLMGALFYALFGKSFPLPGTLPADYSDVRLRQTQNRSLIAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHH
MQESMGDIVVIRNMNRRIVEANRVFREMTGCTSPEGLSCEEIGIAFRPGDRVHAYDVEIA
HHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEECCCCEEEEEEEEEE
TPFGQRIFSWHDVVTRDLASSRLMISSIARDVTEERLALAEREDARLRAEKADAEKARLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHH
ATVSHEIRLPLSGMLGMNHLLSQTKLNQEQRNYLDGMKQSGQSLVQLVEDLLDYSTMEAG
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFRLNPRAENLRRLIESIVEMLAHRAHEKGIEIASFVTPDVPDYLDFDPARLRQVLFNLI
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHH
GNAVKFTQVGGVVIRARMVDGELQITVEDTGPGMSEAEQARIFGEFEQAGPLSQRSAGTG
CCHHHHHHHCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAISARIVREFGGNLTVSSRRGEGSTFKLVFRPGRAPAEMPDSGRSHCLQHTNVLLIA
CHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEC
PEGVAAAVTMEAIEAFGGKCILIHQPEDLDRLRNEPVSRTADLTDIIVDLRISALFRDAL
CCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDWPQQTIRRTLLVSPEERASTTHASFDAWLIRPLREKSLIDVLCGRLRGIERRDAINDN
HHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
HPDVGFSSRVVERASGVEILVAEDDAVNARIIRAVLEKSGYIVRAVDNFQALQQSVASGA
CCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
SRLPNLIVSDLNMPGGEGLDVLPDLLGKLKDERNIPVIILSGDAGAGTAEILLKAGAGAV
HHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCEE
LAKPADPRRLVEEIRRLLEAKRVLSS
EECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELARLRQKVSDGLGLATPAADPADKTTRLANEEHASDVRAVALYVAISLSVGAFAAAL
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALAVPYAVTVVLACFFGAVLMGALFYALFGKSFPLPGTLPADYSDVRLRQTQNRSLIAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHH
MQESMGDIVVIRNMNRRIVEANRVFREMTGCTSPEGLSCEEIGIAFRPGDRVHAYDVEIA
HHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEECCCCEEEEEEEEEE
TPFGQRIFSWHDVVTRDLASSRLMISSIARDVTEERLALAEREDARLRAEKADAEKARLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHH
ATVSHEIRLPLSGMLGMNHLLSQTKLNQEQRNYLDGMKQSGQSLVQLVEDLLDYSTMEAG
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RFRLNPRAENLRRLIESIVEMLAHRAHEKGIEIASFVTPDVPDYLDFDPARLRQVLFNLI
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHH
GNAVKFTQVGGVVIRARMVDGELQITVEDTGPGMSEAEQARIFGEFEQAGPLSQRSAGTG
CCHHHHHHHCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
LGLAISARIVREFGGNLTVSSRRGEGSTFKLVFRPGRAPAEMPDSGRSHCLQHTNVLLIA
CHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEC
PEGVAAAVTMEAIEAFGGKCILIHQPEDLDRLRNEPVSRTADLTDIIVDLRISALFRDAL
CCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDWPQQTIRRTLLVSPEERASTTHASFDAWLIRPLREKSLIDVLCGRLRGIERRDAINDN
HHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
HPDVGFSSRVVERASGVEILVAEDDAVNARIIRAVLEKSGYIVRAVDNFQALQQSVASGA
CCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
SRLPNLIVSDLNMPGGEGLDVLPDLLGKLKDERNIPVIILSGDAGAGTAEILLKAGAGAV
HHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCEE
LAKPADPRRLVEEIRRLLEAKRVLSS
EECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]